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Abstract

Two myxobacterial strains (designated B00001 and B00002) were isolated from forest soil samples collected from Yakushima Island, Kagoshima, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains B00001 and B00002 respectively formed independent branches within the suborders and and were most closely related to DSM 14710 (90.4 % similarity) and DSM 14553 (91.3 %). Neither strain showed typical features of myxobacteria such as bacteriolytic action or fruiting body formation, but both had high DNA G+C contents (66.3–68.3 mol%). Swarming motility was observed in strain B00002 only. Cells of both strains were vegetative, chemoheterotrophic, mesophilic, strictly aerobic, Gram-negative, motile rods, and both strains exhibited esterase lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and β-galactosidase activities. Strain B00001 contained MK-7 as the predominant respiratory quinone and the major fatty acid was iso-C. In contrast, strain B00002 contained MK-8 as the major cellular quinone and the major fatty acids were Cω5 and iso-C. Based on the phenotypic and genotypic data presented, strains B00001 and B00002 represent novel genera and species, for which we propose the names gen. nov., sp. nov. and gen. nov., sp. nov., respectively. The type strains of and are B00001 ( = NBRC 109945 = DSM 27710) and B00002 ( = NBRC 109946 = DSM 27648), respectively. The new genera are assigned to the new families fam. nov. and fam. nov., respectively. In addition, fam. nov., is proposed to accommodate the genus , which is related to the genus .

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2014-10-01
2021-08-04
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