1887

Abstract

Two catalase- and oxidase-negative -like strains, LMG 27682 and LMG 27684, were isolated from raw camel milk in Morocco. Comparative 16S rRNA gene sequencing assigned these bacteria to the genus Streptococcus with 2777-2-07 as their closest phylogenetic neighbour (95.9 % and 95.7 % similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.7 %. Although strains LMG 27682 and LMG 27684 shared a DNA–DNA hybridization value that corresponded to the threshold level for species delineation (68 %), the two strains could be distinguished by multiple biochemical tests, sequence analysis of the phenylalanyl-tRNA synthase (), RNA polymerase () and ATP synthase () genes and by their MALDI-TOF MS profiles. On the basis of these considerable phenotypic and genotypic differences, we propose to classify both strains as novel species of the genus Streptococcus, for which the names sp. nov. (type strain, LMG 27682 = CCMM B831) and sp. nov. (type strain, LMG 27684 = CCMM B833) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062893-0
2014-07-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2480.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062893-0&mimeType=html&fmt=ahah

References

  1. Chanter N.. ( 1997;). Streptococci and enterococci as animal pathogens. . J Appl Microbiol 83: (Symp. Supp.) 100S–109S. [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D., Hutson R. A., Hoyles L., Falsen E., Nikolaitchouk N., Foster G.. ( 2001;). Streptococcus ovis sp. nov., isolated from sheep. . Int J Syst Evol Microbiol 51:, 1147–1150. [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Hutson R. A., Falsen E., Inganäs E., Bisgaard M.. ( 2002;). Streptococcus gallinaceus sp. nov., from chickens. . Int J Syst Evol Microbiol 52:, 1161–1164. [CrossRef][PubMed]
    [Google Scholar]
  4. Devriese L. A., Pot B., Vandamme P., Kersters K., Collins M. D., Alvarez N., Haesebrouck F., Hommez J.. ( 1997;). Streptococcus hyovaginalis sp. nov. and Streptococcus thoraltensis sp. nov., from the genital tract of sows. . Int J Syst Bacteriol 47:, 1073–1077. [CrossRef][PubMed]
    [Google Scholar]
  5. Drummond A. J., Ashton B., Buxton S., Cheung M., Cooper A., Duran C., Field M., Heled J., Kearse M.. & other authors ( 2011;). Geneious V5.4, available from http://www.geneious.com/. .
    [Google Scholar]
  6. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C.. ( 1989;). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. . Nucleic Acids Res 17:, 7843–7853. [CrossRef][PubMed]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  8. Gevers D., Huys G., Swings J.. ( 2001;). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. . FEMS Microbiol Lett 205:, 31–36. [CrossRef][PubMed]
    [Google Scholar]
  9. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K.. ( 1998;). Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44:, 1148–1153. [CrossRef]
    [Google Scholar]
  10. Gouy M., Guindon S., Gascuel O.. ( 2010;). SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. . Mol Biol Evol 27:, 221–224. [CrossRef][PubMed]
    [Google Scholar]
  11. Hardie J. M., Whiley R. A.. ( 1997;). Classification and overview of the genera Streptococcus and Enterococcus. . J Appl Microbiol 83: (Symp. Supp.), 1S–11S. [CrossRef][PubMed]
    [Google Scholar]
  12. Huch M., De Bruyne K., Cleenwerck I., Bub A., Cho G. S., Watzl B., Snauwaert I., Franz C. M. A. P., Vandamme P.. ( 2013;). Streptococcus rubneri sp. nov., isolated from the human throat. . Int J Syst Evol Microbiol 63:, 4026–4032. [CrossRef][PubMed]
    [Google Scholar]
  13. Kilian M.. ( 1998;). Streptococcus and Lactobacillus. . In Topley and Wilson’s Microbiology and Microbial Infections, , 9th edn., vol. 2, pp. 633–667. Edited by Balows A., Duerden B. I... London:: Arnold;.
    [Google Scholar]
  14. Kilian M., Mikkenlsen L., Henrichsen J.. ( 1989;). Taxonomic study of viridans streptococci: description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrewes and Horder 1906). . Int J Syst Bacteriol 39:, 471–484. [CrossRef]
    [Google Scholar]
  15. Kilpper-Bälz R., Schleifer K. H.. ( 1987;). Streptococcus suis sp. nov., nom. rev.. Int J Syst Bacteriol 37:, 160–162. [CrossRef]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  17. Köhler W.. ( 2007;). The present state of species within the genera Streptococcus and Enterococcus. . Int J Med Microbiol 297:, 133–150. [CrossRef][PubMed]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J.. ( 2005a;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151:, 2141–2150. [CrossRef][PubMed]
    [Google Scholar]
  20. Naser S. M., Vancanneyt M., De Graef E., Devriese L. A., Snauwaert C., Lefebvre K., Hoste B., Švec P., Decostere A.. & other authors ( 2005b;). Enterococcus canintestini sp. nov., from faecal samples of healthy dogs. . Int J Syst Evol Microbiol 55:, 2177–2182. [CrossRef][PubMed]
    [Google Scholar]
  21. Niemann S., Pühler A., Tichy H. V., Simon R., Selbitschka W.. ( 1997;). Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. . J Appl Microbiol 82:, 477–484. [CrossRef][PubMed]
    [Google Scholar]
  22. Ouadghiri M., Vancanneyt M., Vandamme P., Naser S., Gevers D., Lefebvre K., Swings J., Amar M.. ( 2009;). Identification of lactic acid bacteria in Moroccan raw milk and traditionally fermented skimmed milk ‘lben’. . J Appl Microbiol 106:, 486–495. [CrossRef][PubMed]
    [Google Scholar]
  23. Parte A. C.. ( 2014;). LPSN–list of prokaryotic names with standing in nomenclature. . Nucleic Acids Res 42: (D1), D613–D616. [CrossRef][PubMed]
    [Google Scholar]
  24. Pitcher D. G., Saunders N. A., Owen R. J.. ( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. . Lett Appl Microbiol 8:, 151–156. [CrossRef]
    [Google Scholar]
  25. Strohalm M., Kavan D., Novák P., Volný M., Havlícek V.. ( 2010;). mMass 3: a cross-platform software environment for precise analysis of mass spectrometric data. . Anal Chem 82:, 4648–4651. [CrossRef][PubMed]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  27. Tappe D., Pukall R., Schumann P., Gronow S., Spiliotis M., Claus H., Brehm K., Vogel U.. ( 2009;). Streptococcus merionis sp. nov., isolated from Mongolian jirds (Meriones unguiculatus). . Int J Syst Evol Microbiol 59:, 766–770. [CrossRef][PubMed]
    [Google Scholar]
  28. Vela A. I., Mentaberre G., Marco I., Velarde R., Lavín S., Domínguez L., Fernández-Garayzábal J. F.. ( 2011a;). Streptococcus rupicaprae sp. nov., isolated from a Pyrenean chamois (Rupicapra pyrenaica). . Int J Syst Evol Microbiol 61:, 1989–1993. [CrossRef][PubMed]
    [Google Scholar]
  29. Vela A. I., Sánchez V., Mentaberre G., Lavín S., Domínguez L., Fernández-Garayzábal J. F.. ( 2011b;). Streptococcus porcorum sp. nov., isolated from domestic and wild pigs. . Int J Syst Evol Microbiol 61:, 1585–1589. [CrossRef][PubMed]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  31. Wieme A., Cleenwerck I., Van Landschoot A., Vandamme P.. ( 2012;). Pediococcus lolii DSM 19927T and JCM 15055T are strains of Pediococcus acidilactici. . Int J Syst Evol Microbiol 62:, 3105–3108. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062893-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062893-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error