1887

Abstract

A Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic bacterium with gliding motility, designated strain SW150, was isolated from surface seawater of the South Pacific Gyre (39° 19′ S 139° 48′ W) during the Integrated Ocean Drilling Program Expedition 329. Optimal growth occurred in the presence of 2–4 % (w/v) NaCl, at pH 7–8 and at 28–30 °C. The dominant fatty acids were iso-C, iso-C 3-OH, iso-C G, Cω6 and/or Cω7 and 10-methyl C and/or iso-Cω9. The polar lipids of strain SW150 comprised phosphatidylethanolamine, three unknown polar lipids and one unknown aminolipid. The major respiratory quinone was MK-6. The DNA G+C content of strain SW150 was 33.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to JAMB N27 and KMM 6020 with 97.8 and 96.8 % sequence similarities, respectively. The estimated DNA–DNA hybridization values were 21.00±2.33 % between strain SW150 and JAMB N27 and 20.60±2.32 % between strain SW150 and XH134. On the basis of polyphasic analyses, strain SW150 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SW150 ( = JCM 18214 = CGMCC 1.12180).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062695-0
2014-06-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/6/1991.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062695-0&mimeType=html&fmt=ahah

References

  1. Auch A. F., Klenk H. P., Göker M.. ( 2010a;). Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. . Stand Genomic Sci 2:, 142–148. [CrossRef][PubMed]
    [Google Scholar]
  2. Auch A. F., von Jan M., Klenk H. P., Göker M.. ( 2010b;). Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. . Stand Genomic Sci 2:, 117–134. [CrossRef][PubMed]
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. (editors) ( 1995;). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  5. Beveridge T. J., Lawrence J. R., Murray R. G. E.. ( 2007;). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 19–33. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  6. Chen W. M., Sheu F. S., Sheu S. Y.. ( 2012;). Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan. . Arch Microbiol 194:, 103–112. [CrossRef][PubMed]
    [Google Scholar]
  7. Collins M. D.. ( 1994;). Isoprenoid quinones. . In Chemical Methods in Prokaryotic Systematics, pp. 265–309. Edited by Goodfellow M., O’Donnell A. G... Chichester:: Wiley;.
    [Google Scholar]
  8. Hsu S. C., Lockwood J. L.. ( 1975;). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29:, 422–426.[PubMed]
    [Google Scholar]
  9. Kennedy J., Margassery L. M., O’Leary N. D., O’Gara F., Morrissey J., Dobson A. D.. ( 2014;). Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum. . Int J Syst Evol Microbiol 64:, 501–505. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  12. Lewin R. A.. ( 1969;). A classification of flexibacteria. . J Gen Microbiol 58:, 189–206. [CrossRef][PubMed]
    [Google Scholar]
  13. Lin B., Lu G., Zheng Y., Xie W., Li S., Hu Z.. ( 2012;). Aquimarina agarilytica sp. nov., an agarolytic species isolated from a red alga. . Int J Syst Evol Microbiol 62:, 869–873. [CrossRef][PubMed]
    [Google Scholar]
  14. Lyman J., Fleming R. H.. ( 1940;). Composition of seawater. . J Mar Res 3:, 134–146.
    [Google Scholar]
  15. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M.. ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. . BMC Bioinformatics 14:, 60. [CrossRef][PubMed]
    [Google Scholar]
  16. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Miyazaki M., Nagano Y., Fujiwara Y., Hatada Y., Nogi Y.. ( 2010;). Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses. . Int J Syst Evol Microbiol 60:, 2298–2302. [CrossRef][PubMed]
    [Google Scholar]
  19. Montero-Calasanz M. del C., Göker M., Rohde M., Spröer C., Schumann P., Busse H.-J., Schmid M., Tindall B. J., Klenk H.-P., Camacho M.. ( 2013;). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. . Int J Syst Evol Microbiol 63:, 4386–4395. [CrossRef][PubMed]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S.. ( 2005;). Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov.. Int J Syst Evol Microbiol 55:, 225–229. [CrossRef][PubMed]
    [Google Scholar]
  21. Nedashkovskaya O. I., Vancanneyt M., Christiaens L., Kalinovskaya N. I., Mikhailov V. V., Swings J.. ( 2006;). Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina. . Int J Syst Evol Microbiol 56:, 2037–2041. [CrossRef][PubMed]
    [Google Scholar]
  22. Park S. C., Choe H. N., Baik K. S., Seong C. N.. ( 2012;). Aquimarina mytili sp. nov., isolated from the gut microflora of a mussel, Mytilus coruscus, and emended description of Aquimarina macrocephali. . Int J Syst Evol Microbiol 62:, 1974–1979. [CrossRef][PubMed]
    [Google Scholar]
  23. Park S. C., Choe H. N., Baik K. S., Seong C. N.. ( 2013;). Aquimarina gracilis sp. nov., isolated from the gut microflora of a mussel, Mytilus coruscus, and emended description of Aquimarina spongiae. . Int J Syst Evol Microbiol 63:, 1782–1787. [CrossRef][PubMed]
    [Google Scholar]
  24. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  27. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  29. Yi H., Chun J.. ( 2011;). Aquimarina addita sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61:, 2445–2449. [CrossRef][PubMed]
    [Google Scholar]
  30. Yoon J. H., Kang S. J., Jung S. Y., Oh H. W., Oh T. K.. ( 2006;). Gaetbulimicrobium brevivitae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 56:, 115–119. [CrossRef][PubMed]
    [Google Scholar]
  31. Yoon B. J., You H. S., Lee D. H., Oh D. C.. ( 2011;). Aquimarina spongiae sp. nov., isolated from marine sponge Halichondria oshoro. . Int J Syst Evol Microbiol 61:, 417–421. [CrossRef][PubMed]
    [Google Scholar]
  32. Yu T., Yin Q., Song X., Zhao R., Shi X., Zhang X. H.. ( 2013;). Aquimarina longa sp. nov., isolated from seawater, and emended description of Aquimarina muelleri. . Int J Syst Evol Microbiol 63:, 1235–1240. [CrossRef][PubMed]
    [Google Scholar]
  33. Yu T., Zhang Z., Fan X., Shi X., Zhang X. H.. ( 2014;). Aquimarina megaterium sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 64:, 122–127. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062695-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062695-0
Loading

Data & Media loading...

Supplements

Supplementary Material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error