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Volume 64, Issue Pt_9

sp. nov. and sp. nov., moderately halophilic bacteria isolated from seawater Free

Abstract

Two Gram-stain-negative, aerobic, moderately halophilic, rod-shaped bacteria (strains Ar-45 and DY470) were isolated from seawater collected from the Southern Ocean and the Pacific Ocean, respectively. Growth of strain Ar-45 was observed with between 0.5 and 10.0 % (w/v) NaCl (optimally with 0.5–3.0 %) and between pH 5.5 and 9.5. Strain DY470 grew in the presence of 0.5–7.5 % (w/v) NaCl (optimally with 2.0 %) and at pH 5.5–8.5. Chemotaxonomic analysis showed Q-10 as the respiratory quinone for both strains. The major fatty acids (>5 %) of strain Ar-45 were C, C cyclo ω8 and Cω7, while those of strain DY470 were Cω7, C and 11-methyl Cω7. The DNA G+C contents of the two strains were 62.0 and 61.8 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains Ar-45 and DY470 were related most closely to the genus , with sequence similarities of 97.4–94.0 and 97.7–94.7 %, respectively. The DNA–DNA hybridization value between strain Ar-45 and LMG 23705 was 22.0 %. Levels of DNA–DNA relatedness between strain DY470 and LMG 24663 and DSM 15984 were 32.5 and 26.1 %, respectively. Based on phylogenetic, chemotaxonomic and phenotypic data, strains Ar-45 and DY470 are considered to represent two novel species of the genus , for which the names (type strain Ar-45 = CGMCC 1.12662 = LMG 27868) and (type strain DY470 = CGMCC 1.12664 = LMG 27871) are proposed.

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Funding
This study was supported by the:
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-14-E-02 and DY125-15-R-03)
  • National Natural Science Foundation of China (Award 41276173)
  • Zhejiang Provincial Natural Science Foundation of China (Award LQ13D060002)
  • Scientific Research Fund of the Second Institute of Oceanography (Award JT1305)
© 2014 IUMS
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2014-09-01
2024-05-03
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References

  1. Chen M.-H., Sheu S.-Y., Chen C. A., Wang J.-T., Chen W.-M. ( 2012 ). Roseivivax isoporae sp. nov., isolated from a reef-building coral, and emended description of the genus Roseivivax . . Int J Syst Evol Microbiol 62, 1259–1264. [View Article] [PubMed]
    [Google Scholar]
  2. Cho J.-C., Giovannoni S. J. ( 2004 ). Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. . Int J Syst Evol Microbiol 54, 1129–1136. [View Article] [PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  4. Dong X.-Z., Cai M.-Y. ( 2001 ). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press (English translation);.
     [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  6. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  7. Gu J., Guo B., Wang Y.-N., Yu S.-L., Inamori R., Qu R., Ye Y.-G., Wu X.-L. ( 2007 ). Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. . Int J Syst Evol Microbiol 57, 157–160. [View Article] [PubMed]
    [Google Scholar]
  8. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  9. Iwaki H., Yasukawa N., Fujioka M., Takada K., Hasegawa Y. ( 2013 ). Isolation and characterization of a marine cyclohexylacetate-degrading bacterium Lutimaribacter litoralis sp. nov., and reclassification of Oceanicola pacificus as Lutimaribacter pacificus comb. nov.. Curr Microbiol 66, 588–593. [View Article] [PubMed]
    [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  11. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  12. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 1183–1184.[PubMed]
    [Google Scholar]
  13. Lin K.-Y., Sheu S.-Y., Chang P.-S., Cho J.-C., Chen W.-M. ( 2007 ). Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. . Int J Syst Evol Microbiol 57, 1625–1629. [View Article] [PubMed]
    [Google Scholar]
  14. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  15. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297–306. [View Article] [PubMed]
    [Google Scholar]
  16. Mikhailov V. V., Romanenko L. A., Ivanova E. P. ( 2006 ). The genus Alteromonas and related proteobacteria. . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 6, pp. 597–645. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  17. Park S., Lee M.-H., Yoon J.-H. ( 2013 ). Oceanicola litoreus sp. nov., an alphaproteobacterium isolated from the seashore sediment. . Antonie van Leeuwenhoek 103, 859–866. [View Article] [PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  20. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 4673–4680. [View Article] [PubMed]
    [Google Scholar]
  21. Wu Y.-H., Yu P.-S., Zhou Y.-D., Xu L., Wang C.-S., Wu M., Oren A., Xu X.-W. ( 2013 ). Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater. . Int J Syst Evol Microbiol 63, 3451–3456. [View Article] [PubMed]
    [Google Scholar]
  22. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. ( 2007 ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. . Int J Syst Evol Microbiol 57, 1619–1624. [View Article] [PubMed]
    [Google Scholar]
  23. Xu X.-W., Huo Y.-Y., Bai X.-D., Wang C.-S., Oren A., Li S.-Y., Wu M. ( 2011 ). Kordiimonas lacus sp. nov., isolated from a ballast water tank, and emended description of the genus Kordiimonas . . Int J Syst Evol Microbiol 61, 422–426. [View Article] [PubMed]
    [Google Scholar]
  24. Yuan J., Lai Q., Wang B., Sun F., Liu X., Du Y., Li G., Gu L., Zheng T., Shao Z. ( 2009 ). Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. . Int J Syst Evol Microbiol 59, 1158–1161. [View Article] [PubMed]
    [Google Scholar]
  25. Zheng Q., Chen C., Wang Y.-N., Jiao N. ( 2010 ). Oceanicola nitratireducens sp. nov., a marine alphaproteobacterium isolated from the South China Sea. . Int J Syst Evol Microbiol 60, 1655–1659. [View Article] [PubMed]
    [Google Scholar]
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Oceanicolaantarcticus sp. nov. and Oceanicola flagellatus sp. nov., moderately halophilic bacteria isolated from seawater
Int J Syst Evol Microbiol 64, 2975 (2014); https://doi.org/10.1099/ijs.0.062588-0
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