1887

Abstract

Two Gram-stain-negative, aerobic, moderately halophilic, rod-shaped bacteria (strains Ar-45 and DY470) were isolated from seawater collected from the Southern Ocean and the Pacific Ocean, respectively. Growth of strain Ar-45 was observed with between 0.5 and 10.0 % (w/v) NaCl (optimally with 0.5–3.0 %) and between pH 5.5 and 9.5. Strain DY470 grew in the presence of 0.5–7.5 % (w/v) NaCl (optimally with 2.0 %) and at pH 5.5–8.5. Chemotaxonomic analysis showed Q-10 as the respiratory quinone for both strains. The major fatty acids (>5 %) of strain Ar-45 were C, C cyclo ω8 and Cω7, while those of strain DY470 were Cω7, C and 11-methyl Cω7. The DNA G+C contents of the two strains were 62.0 and 61.8 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains Ar-45 and DY470 were related most closely to the genus , with sequence similarities of 97.4–94.0 and 97.7–94.7 %, respectively. The DNA–DNA hybridization value between strain Ar-45 and LMG 23705 was 22.0 %. Levels of DNA–DNA relatedness between strain DY470 and LMG 24663 and DSM 15984 were 32.5 and 26.1 %, respectively. Based on phylogenetic, chemotaxonomic and phenotypic data, strains Ar-45 and DY470 are considered to represent two novel species of the genus , for which the names (type strain Ar-45 = CGMCC 1.12662 = LMG 27868) and (type strain DY470 = CGMCC 1.12664 = LMG 27871) are proposed.

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2014-09-01
2019-11-22
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