1887

Abstract

Halophilic archaeal strain YGHS32 was isolated from the Yinggehai marine solar saltern near Shanya city of Hainan Province, China. Cells of the strain were pleomorphic and lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. Strain YGHS32 was able to grow at 20–50 °C (optimum 37 °C), in the presence of 0.9–4.8 M NaCl (optimum 2.1 M NaCl), with 0.005–1.0 M MgCl (optimum 0.3 M MgCl) and at pH 6.0–8.5 (optimum pH 7.5). The minimal NaCl concentration to prevent cell lysis was 5 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and four major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether, mannosyl glucosyl diether, glucosyl mannosyl glucosyl diether and a diglycosyl diether. Strain YGHS32 had two dissimilar 16S rRNA genes and both of them were phylogenetically related to those of JCM 17820 (92.9–96.3 % sequence similarity). The ′ gene sequence similarity between strain YGHS32 and JCM 17820 was 91.3 %. The DNA G+C content of strain YGHS32 was 64.0 mol%. The DNA–DNA hybridization value between strain YGHS32 and JCM 17820 was 45 %. It was concluded that strain YGHS32 ( = CGMCC 1.12129 = JCM 18640) represents a novel species of the genus , for which the name sp. nov. is proposed. An emended description of the genus is also presented.

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2014-05-01
2019-10-18
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References

  1. Cui H.-L., Zhou P.-J., Oren A., Liu S.-J.. ( 2009;). Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. . Extremophiles 13:, 31–37. [CrossRef][PubMed]
    [Google Scholar]
  2. Cui H.-L., Gao X., Yang X., Xu X.-W.. ( 2010;). Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. . Extremophiles 14:, 493–499. [CrossRef][PubMed]
    [Google Scholar]
  3. Cui H.-L., Yang X., Mou Y. Z.. ( 2011;). Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria. . Extremophiles 15:, 625–631. [CrossRef][PubMed]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  5. Echigo A., Minegishi H., Shimane Y., Kamekura M., Itoh T., Usami R.. ( 2013;). Halomicroarcula pellucida gen. nov., sp. nov., a non-pigmented, transparent-colony-forming, halophilic archaeon isolated from solar salt. . Int J Syst Evol Microbiol 63:, 3556–3562. [CrossRef][PubMed]
    [Google Scholar]
  6. Huß V. A., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  7. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  8. Minegishi H., Kamekura M., Itoh T., Echigo A., Usami R., Hashimoto T.. ( 2010;). Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B′ (rpoB′) gene. . Int J Syst Evol Microbiol 60:, 2398–2408. [CrossRef][PubMed]
    [Google Scholar]
  9. Namwong S., Tanasupawat S., Kudo T., Itoh T.. ( 2011;). Haloarcula salaria sp. nov. and Haloarcula tradensis sp. nov., isolated from salt in Thai fish sauce. . Int J Syst Evol Microbiol 61:, 231–236. [CrossRef][PubMed]
    [Google Scholar]
  10. Oren A., Ventosa A., Grant W. D.. ( 1997;). Proposed minimal standards for description of new taxa in the order Halobacteriales. . Int J Syst Bacteriol 47:, 233–238. [CrossRef]
    [Google Scholar]
  11. Oren A., Arahal D. R., Ventosa A.. ( 2009;). Emended descriptions of genera of the family Halobacteriaceae. . Int J Syst Evol Microbiol 59:, 637–642. [CrossRef][PubMed]
    [Google Scholar]
  12. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  13. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  14. Yang X., Cui H.-L.. ( 2012;). Halomicrobium zhouii sp. nov., a halophilic archaeon from a marine solar saltern. . Int J Syst Evol Microbiol 62:, 1235–1240. [CrossRef][PubMed]
    [Google Scholar]
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