1887

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, BS-B2, which was isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-B2 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The novel strain exhibited highest 16S rRNA gene sequence similarity (97.4 %) to DPG-138. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BS-B2 is closely related to KMM 9018, showing 96.5 % sequence similarity. Strain BS-B2 contained Q-10 as the predominant ubiquinone and Cω7 as the predominant fatty acid. The polar lipid profile of strain BS-B2 comprised phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid as major components, and differentiated it from the type strains of and . The DNA G+C content of strain BS-B2 was 62.2 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain BS-B2 can be distinguished from phylogenetically related genera as well as and . On the basis of the data presented, strain BS-B2 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is BS-B2 ( = KCTC 42052 = CECT 8532).

Funding
This study was supported by the:
  • , National Institute of Biological Resources (NIBR)
  • , Ministry of Environment (MOE)
  • , Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea , (Award NRF-2013M3A9A5075953)
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2014-09-01
2021-01-24
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