1887

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, BS-B2, which was isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-B2 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The novel strain exhibited highest 16S rRNA gene sequence similarity (97.4 %) to DPG-138. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BS-B2 is closely related to KMM 9018, showing 96.5 % sequence similarity. Strain BS-B2 contained Q-10 as the predominant ubiquinone and Cω7 as the predominant fatty acid. The polar lipid profile of strain BS-B2 comprised phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid as major components, and differentiated it from the type strains of and . The DNA G+C content of strain BS-B2 was 62.2 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain BS-B2 can be distinguished from phylogenetically related genera as well as and . On the basis of the data presented, strain BS-B2 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is BS-B2 ( = KCTC 42052 = CECT 8532).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062406-0
2014-09-01
2019-11-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/9/2969.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062406-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef] [PubMed]
    [Google Scholar]
  3. Buchan A. , González J. M. , Moran M. A. . ( 2005; ). Overview of the marine Roseobacter lineage. . Appl Environ Microbiol 71:, 5665–5677. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cho J.-C. , Giovannoni S. J. . ( 2006; ). Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales . . Int J Syst Evol Microbiol 56:, 855–859. [CrossRef] [PubMed]
    [Google Scholar]
  5. Euzéby J. P. . ( 1997; ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47:, 590–592 (List of Prokaryotic names with Standing in Nomenclature. URL: http://www.bacterio.net).[CrossRef]
    [Google Scholar]
  6. Garrity G. M. , Bell J. A. , Lilburn T. . ( 2005; ). Order III. Rhodobacterales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, part C, p. 161. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  7. Garrity G. M. , Bell J. A. , Lilburn T. . ( 2006; ). List of new names and new combinations previously effectively, but not validly, published. . Int J Syst Evol Microbiol 56:, 1–6. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hahnke S. , Tindall B. J. , Schumann P. , Simon M. , Brinkhoff T. . ( 2013; ). Pelagimonas varians gen. nov., sp. nov., isolated from the southern North Sea. . Int J Syst Evol Microbiol 63:, 835–843. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hameed A. , Shahina M. , Lin S.-Y. , Lai W.-A. , Hsu Y.-H. , Liu Y.-C. , Huang Y.-M. , Young C.-C. . ( 2013; ). Shimia biformata sp. nov., isolated from surface seawater, and emended description of the genus Shimia Choi and Cho 2006. . Int J Syst Evol Microbiol 63:, 4533–4539. [CrossRef] [PubMed]
    [Google Scholar]
  10. Hwang C. Y. , Bae G. D. , Yih W. , Cho B. C. . ( 2009; ). Marivita cryptomonadis gen. nov., sp. nov. and Marivita litorea sp. nov., of the family Rhodobacteraceae, isolated from marine habitats. . Int J Syst Evol Microbiol 59:, 1568–1575. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim Y.-G. , Hwang C. Y. , Cho B. C. . ( 2008; ). Pelagicola litoralis gen. nov., sp. nov., isolated from coastal water in Korea. . Int J Syst Evol Microbiol 58:, 2102–2104. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim Y.-O. , Kong H. J. , Park S. , Kang S.-J. , Kim W.-J. , Kim K.-K. , Oh T.-K. , Yoon J.-H. . ( 2012; ). Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi . . Int J Syst Evol Microbiol 62:, 931–936. [CrossRef] [PubMed]
    [Google Scholar]
  13. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  14. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  15. Leifson E. . ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  16. Lucena T. , Pujalte M. J. , Ruvira M. A. , Garay E. , Macián M. C. , Arahal D. R. . ( 2012; ). Tropicibacter multivorans sp. nov., an aerobic alphaproteobacterium isolated from surface seawater. . Int J Syst Evol Microbiol 62:, 844–848. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lucena T. , Ruvira M. A. , Macián M. C. , Pujalte M. J. , Arahal D. R. . ( 2013; ). Description of Tropicibacter mediterraneus sp. nov. and Tropicibacter litoreus sp. nov.. Syst Appl Microbiol 36:, 325–329. [CrossRef] [PubMed]
    [Google Scholar]
  18. Minnikin D. E. , Patel P. V. , Alshamaony L. , Goodfellow M. . ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  20. Park S. , Lee M.-H. , Lee J.-S. , Oh T.-K. , Yoon J.-H. . ( 2012; ). Thalassobius maritimus sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 62:, 8–12. [CrossRef] [PubMed]
    [Google Scholar]
  21. Rappé M. S. , Vergin K. , Giovannoni S. J. . ( 2000; ). Phylogenetic comparisons of a coastal bacterioplankton community with its counterparts in open ocean and freshwater systems. . FEMS Microbiol Ecol 33:, 219–232. [CrossRef] [PubMed]
    [Google Scholar]
  22. Romanenko L. A. , Tanaka N. , Svetashev V. I. , Mikhailov V. V. . ( 2011; ). Primorskyibacter sedentarius gen. nov., sp. nov., a novel member of the class Alphaproteobacteria from shallow marine sediments. . Int J Syst Evol Microbiol 61:, 1572–1578. [CrossRef] [PubMed]
    [Google Scholar]
  23. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  24. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  25. Vandecandelaere I. , Nercessian O. , Segaert E. , Achouak W. , Mollica A. , Faimali M. , Vandamme P. . ( 2009; ). Nautella italica gen. nov., sp. nov., isolated from a marine electroactive biofilm. . Int J Syst Evol Microbiol 59:, 811–817. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yoon J.-H. , Kim H. , Kim S.-B. , Kim H.-J. , Kim W. Y. , Lee S. T. , Goodfellow M. , Park Y.-H. . ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  27. Yoon J.-H. , Lee S. T. , Park Y.-H. . ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yoon J.-H. , Kang K. H. , Park Y.-H. . ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yoon J.-H. , Kang S.-J. , Lee J.-S. , Oh T.-K. . ( 2009; ). Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea. . Int J Syst Evol Microbiol 59:, 48–52. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yoon J.-H. , Kang S.-J. , Lee S.-Y. , Jung Y.-T. , Lee J.-S. , Oh T.-K. . ( 2012; ). Marivita hallyeonensis sp. nov., isolated from seawater, reclassification of Gaetbulicola byunsanensis as Marivita byunsanensis comb. nov. and emended description of the genus Marivita Hwang et al. 2009. . Int J Syst Evol Microbiol 62:, 839–843. [CrossRef] [PubMed]
    [Google Scholar]
  31. Yoon J.-H. , Kang S.-J. , Lee J.-S. . ( 2013a; ). Marivita geojedonensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 63:, 423–427. [CrossRef] [PubMed]
    [Google Scholar]
  32. Yoon J.-H. , Lee S.-Y. , Jung Y.-T. , Lee J.-S. , Lee K.-C. . ( 2013b; ). Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment. . Int J Syst Evol Microbiol 63:, 2025–2031. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062406-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062406-0
Loading

Data & Media loading...

Supplements

Supplementary Material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error