1887

Abstract

A Gram-stain-negative, facultatively anaerobic, non-motile and coccoid- to short-rod-shaped bacterium, designated strain Dys-CH1, was isolated from the hindgut of a fungus-growing termite . The optimal pH and cultivation temperature of strain Dys-CH1 were pH 7.2–7.6 and 35–37 °C, respectively. Sequence analysis of 16S rRNA gene showed that Dys-CH1 shared 94.6 % and 90.9 % similarity with JCM 16697 and CCUG 42882, respectively. Strain Dys-CH1 was found to be different from other species of the genus with validly published names with respect to taxonomically important traits, including habitat, biochemical tests, DNA G+C content, bile resistance, fatty-acid composition and susceptibility to antimicrobial agents. On the basis of these characteristics, strain Dys-CH1 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is Dys-CH1 ( = JCM 19375 = DSM 27370).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2011CB707402)
  • National Natural Science Foundation of China (Award 30870085 and 31272370)
  • Key Laboratory of Biofuel, Chinese Academy of Sciences (Award CASKLB201305)
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2014-09-01
2021-10-18
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References

  1. Debelian G. J., Olsen I., Tronstad L. ( 1997 ). Distinction of Prevotella intermedia and Prevotella nigrescens from endodontic bacteremia through their fatty acid contents. . Anaerobe 3, 6168. [View Article] [PubMed]
    [Google Scholar]
  2. Hofstad T., Olsen I., Eribe E. R., Falsen E., Collins M. D., Lawson P. A. ( 2000 ). Dysgonomonas gen. nov. to accommodate Dysgonomonas gadei sp. nov., an organism isolated from a human gall bladder, and Dysgonomonas capnocytophagoides (formerly CDC group DF-3). . Int J Syst Evol Microbiol 50, 21892195. [View Article] [PubMed]
    [Google Scholar]
  3. Jordan E. M., Thompson F. L., Zhang X.-H., Li Y., Vancanneyt M., Kroppenstedt R. M., Priest F. G., Austin B. ( 2007 ). Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China. . Int J Syst Evol Microbiol 57, 114121. [CrossRef]
    [Google Scholar]
  4. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  5. Kodama Y., Shimoyama T., Watanabe K. ( 2012 ). Dysgonomonas oryzarvi sp. nov., isolated from a microbial fuel cell. . Int J Syst Evol Microbiol 62, 30553059. [View Article] [PubMed]
    [Google Scholar]
  6. Lawson P. A., Falsen E., Inganäs E., Weyant R. S., Collins M. D. ( 2002 ). Dysgonomonas mossii sp. nov., from human sources. . Syst Appl Microbiol 25, 194197. [View Article] [PubMed]
    [Google Scholar]
  7. Lawson P. A., Carlson P., Wernersson S., Moore E. R., Falsen E. ( 2010 ). Dysgonomonas hofstadii sp. nov., isolated from a human clinical source. . Anaerobe 16, 161164. [View Article] [PubMed]
    [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic by high performance liquid chromatography. . Int J Syst Evol Microbiol 39, 159167.
    [Google Scholar]
  9. Moore L. V., Bourne D. M., Moore W. E. ( 1994 ). Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic Gram-negative bacilli. . Int J Syst Bacteriol 44, 338347. [View Article] [PubMed]
    [Google Scholar]
  10. Nagai F., Morotomi M., Watanabe Y., Sakon H., Tanaka R. ( 2010 ). Alistipes indistinctus sp. nov. and Odoribacter laneus sp. nov., common members of the human intestinal microbiota isolated from faeces. . Int J Syst Evol Microbiol 60, 12961302. [View Article] [PubMed]
    [Google Scholar]
  11. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  12. Shinzato N., Muramatsu M., Matsui T., Watanabe Y. ( 2007 ). Phylogenetic analysis of the gut bacterial microflora of the fungus-growing termite Odontotermes formosanus . . Biosci Biotechnol Biochem 71, 906915. [View Article] [PubMed]
    [Google Scholar]
  13. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607655. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  14. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
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