1887

Abstract

Halophilic archaeal strain GX10 was isolated from the Gangxi marine solar saltern in China. Strain GX10 was observed to have pleomorphic cells that lysed in distilled water, stained Gram-negative and produced red-pigmented colonies. Strain GX10 was able to grow at 20–50 °C (optimum 37 °C), with 1.4–4.8 M NaCl (optimum 3.1 M NaCl), with 0–0.7 M MgCl (optimum 0.05 M MgCl) and at pH 5.0–9.0 (optimum pH 7.0). The major polar lipids of strain GX10 were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, and five unidentified glycolipids. Phylogenetic tree reconstructions based on 16S rRNA gene and sequences revealed that strain GX10 was distinct from the related genera, , , , , , and . The DNA G+C content of strain GX10 was 62.9 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain GX10 represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is GX10 ( = CGMCC 1.10385 = JCM 17116).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.061606-0
2014-06-01
2019-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/6/2029.html?itemId=/content/journal/ijsem/10.1099/ijs.0.061606-0&mimeType=html&fmt=ahah

References

  1. Burns D. G., Janssen P. H., Itoh T., Kamekura M., Echigo A., Dyall-Smith M. L.. ( 2010;). Halonotius pteroides gen. nov., sp. nov., an extremely halophilic archaeon recovered from a saltern crystallizer. . Int J Syst Evol Microbiol 60:, 1196–1199. [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  3. Cui H.-L., Zhou P.-J., Oren A., Liu S.-J.. ( 2009;). Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. . Extremophiles 13:, 31–37. [CrossRef][PubMed]
    [Google Scholar]
  4. Cui H.-L., Gao X., Yang X., Xu X.-W.. ( 2010;). Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. . Extremophiles 14:, 493–499. [CrossRef][PubMed]
    [Google Scholar]
  5. Cui H.-L., Yang X., Gao X., Xu X.-W.. ( 2011a;). Halogranum gelatinilyticum sp. nov. and Halogranum amylolyticum sp. nov., isolated from a marine solar saltern, and emended description of the genus Halogranum. . Int J Syst Evol Microbiol 61:, 911–915. [CrossRef][PubMed]
    [Google Scholar]
  6. Cui H.-L., Yang X., Mou Y.-Z.. ( 2011b;). Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria. . Extremophiles 15:, 625–631. [CrossRef][PubMed]
    [Google Scholar]
  7. Kim K. K., Lee K. C., Lee J.-S.. ( 2011;). Halogranum salarium sp. nov., a halophilic archaeon isolated from sea salt. . Syst Appl Microbiol 34:, 576–580. [CrossRef][PubMed]
    [Google Scholar]
  8. Liu B.-B., Tang S.-K., Cui H.-L., Zhang Y.-G., Li L., Zhang Y.-M., Zhang L.-L., Li W.-J.. ( 2013;). Halopelagius fulvigenes sp. nov., a halophilic archaeon isolated from a lake. . Int J Syst Evol Microbiol 63:, 2192–2196. [CrossRef][PubMed]
    [Google Scholar]
  9. Mancinelli R. L., Landheim R., Sánchez-Porro C., Dornmayr-Pfaffenhuemer M., Gruber C., Legat A., Ventosa A., Radax C., Ihara K.. & other authors ( 2009;). Halorubrum chaoviator sp. nov., a haloarchaeon isolated from sea salt in Baja California, Mexico, Western Australia and Naxos, Greece. . Int J Syst Evol Microbiol 59:, 1908–1913. [CrossRef][PubMed]
    [Google Scholar]
  10. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  11. Minegishi H., Kamekura M., Itoh T., Echigo A., Usami R., Hashimoto T.. ( 2010;). Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B (rpoB) gene. . Int J Syst Evol Microbiol 60:, 2398–2408. [CrossRef][PubMed]
    [Google Scholar]
  12. Oh D., Porter K., Russ B., Burns D., Dyall-Smith M.. ( 2010;). Diversity of Haloquadratum and other haloarchaea in three, geographically distant, Australian saltern crystallizer ponds. . Extremophiles 14:, 161–169. [CrossRef][PubMed]
    [Google Scholar]
  13. Oren A.. ( 2012;). Taxonomy of the family Halobacteriaceae: a paradigm for changing concepts in prokaryote systematics. . Int J Syst Evol Microbiol 62:, 263–271. [CrossRef][PubMed]
    [Google Scholar]
  14. Oren A., Ventosa A., Grant W. D.. ( 1997;). Proposed minimal standards for description of new taxa in the order Halobacteriales. . Int J Syst Bacteriol 47:, 233–238. [CrossRef]
    [Google Scholar]
  15. Oren A., Arahal D. R., Ventosa A.. ( 2009;). Emended descriptions of genera of the family Halobacteriaceae. . Int J Syst Evol Microbiol 59:, 637–642. [CrossRef][PubMed]
    [Google Scholar]
  16. Qiu X.-X., Mou Y.-Z., Zhao M.-L., Zhang W.-J., Han D., Ren M., Cui H.-L.. ( 2013a;). Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China. . Int J Syst Evol Microbiol 63:, 3975–3980. [CrossRef][PubMed]
    [Google Scholar]
  17. Qiu X.-X., Zhao M.-L., Han D., Zhang W.-J., Cui H.-L.. ( 2013b;). Haloplanus salinus sp. nov., an extremely halophilic archaeon from a Chinese marine solar saltern. . Arch Microbiol 195:, 799–803. [CrossRef][PubMed]
    [Google Scholar]
  18. Qiu X.-X., Zhao M.-L., Han D., Zhang W.-J., Cui H.-L.. ( 2013c;). Halorubrum rubrum sp. nov., an extremely halophilic archaeon from a Chinese salt lake. . Antonie van Leeuwenhoek 104:, 885–891. [CrossRef][PubMed]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  20. Yang X., Cui H.-L.. ( 2012;). Halomicrobium zhouii sp. nov., a halophilic archaeon from a marine solar saltern. . Int J Syst Evol Microbiol 62:, 1235–1240. [CrossRef][PubMed]
    [Google Scholar]
  21. Zhang X., Zhang W.-Y., Shen A.-H., Huo Y.-Y., Zhu X.-F., Wu M.. ( 2013;). Halopelagius longus sp. nov., a member of the family Halobacteriaceae isolated from a salt mine, and emended description of the genus Halopelagius. . Int J Syst Evol Microbiol 63:, 3585–3590. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.061606-0
Loading
/content/journal/ijsem/10.1099/ijs.0.061606-0
Loading

Data & Media loading...

Supplements

Supplementary Material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error