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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_10

sp. nov., an exoelectrogenic microbe in bioelectrochemical systems Free

Abstract

A previously isolated exoelectrogenic bacterium, strain SD-1, was further characterized and identified as a representative of a novel species of the genus . Strain SD-1 was Gram-negative, aerotolerant, anaerobic, non-spore-forming, non-fermentative and non-motile. Cells were short, curved rods (0.8–1.3 µm long and 0.3 µm in diameter). Growth of strain SD-1 was observed at 15–42 °C and pH 6.0–8.5, with optimal growth at 30–35 °C and pH 7. Analysis of 16S rRNA gene sequences indicated that the isolate was a member of the genus , with the closest known relative being PCA (98 % similarity). Similar to other members of the genus , strain SD-1 used soluble or insoluble Fe(III) as the sole electron acceptor coupled with the oxidation of acetate. However, SD-1 could not reduce fumarate as an electron acceptor with acetate oxidization, which is an important physiological trait for . Moreover, SD-1 could grow in media containing as much as 3 % NaCl, while PCA can tolerate just half this concentration, and this difference in salt tolerance was even more obvious when cultivated in bioelectrochemical systems. DNA–DNA hybridization analysis of strain SD-1 and its closest relative, ATCC 51573, showed a relatedness of 61.6 %. The DNA G+C content of strain SD-1 was 58.9 mol%. Thus, on the basis of these characteristics, strain SD-1 was not assigned to , and was instead classified in the genus as a representative of a novel species. The name sp. nov. is proposed, with the type strain SD-1 ( = CGMCC 1.12536 = KCTC 4672).

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Funding
This study was supported by the:
  • King Abdullah University of Science and Technology (KAUST) (Award KUS-I1-003-13)
  • China Postdoctoral Science Foundation (Award 2014T70573 and 2013M541773)
  • National Science Foundation for Distinguished Young Scholars (Award 51225802)
  • Science Fund for Creative Research Groups of the National Natural Science Foundation of China (Award 51121062)
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2014-10-01
2025-01-21
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Geobacter anodireducens sp. nov., an exoelectrogenic microbe in bioelectrochemical systems
Int J Syst Evol Microbiol 64, 3485 (2014); https://doi.org/10.1099/ijs.0.061598-0
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