1887

Abstract

A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (strain 280) isolated from a chicken was studied for its taxonomic allocation. 16S rRNA gene sequence analyses clearly allocated the isolate in the genus group with a 16S rRNA gene sequence similarity of 98.8 % to the currently recognized species, and . This allocation was confirmed by the fatty acid data (major fatty acids: Cω7 and C cyclo ω8) and a polyamine pattern with the major compound putrescine and relatively high amounts of spermidine. Also, the polar lipid profile with phosphatidylethanolamine, phosphatiylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and the genus-specific ‘stretched aminolipid’ was well in line with the description of the genus . The quinone system consisted predominantly of ubiquinone Q-10 with traces of Q-9 and Q-11. DNA–DNA hybridization of strain 280 with Sa25 and KMM 3858 showed relatedness values of 38.8 % (reciprocal 20.2 %) and 30.2 % (reciprocal 29.8 %), respectively. These results in combination with differentiating physiological and biochemical data clearly showed that strain 280 merits species status. We propose the name sp. nov. to accommodate this strain with the type strain 280 ( = LMG 28095 = CIP 110700).

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2014-05-01
2020-01-20
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J.. ( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47:, 39–52. [CrossRef]
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F.. ( 1978;). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef][PubMed]
    [Google Scholar]
  3. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. . Syst Appl Microbiol 11:, 1–8. [CrossRef]
    [Google Scholar]
  4. Busse H.-J., Bunka S., Hensel A., Lubitz W.. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47:, 698–708. [CrossRef]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. ( 2005;). phylip (phylogeny inference package) version 3.6. . Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. (editors) ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Glaeser S. P., Falsen E., Martin K., Kämpfer P.. ( 2013;). Alicyclobacillus consociatus sp. nov., isolated from a human clinical specimen. . Int J Syst Evol Microbiol 63:, 3623–3627. [CrossRef][PubMed]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef][PubMed]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of the protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Steiof M., Dott W.. ( 1991;). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21:, 227–251. [CrossRef][PubMed]
    [Google Scholar]
  13. Kämpfer P., Rosselló-Mora R., Scholz H. C., Welinder-Olsson C., Falsen E., Busse H. J.. ( 2006;). Description of Pseudochrobactrum gen. nov., with the two species Pseudochrobactrum asaccharolyticum sp. nov. and Pseudochrobactrum saccharolyticum sp. nov.. Int J Syst Evol Microbiol 56:, 1823–1829. [CrossRef][PubMed]
    [Google Scholar]
  14. Kämpfer P., Scholz H., Huber B., Thummes K., Busse H.-J., Maas E. W., Falsen E.. ( 2007;). Description of Pseudochrobactrum kiredjianiae sp. nov.. Int J Syst Evol Microbiol 57:, 755–760. [CrossRef][PubMed]
    [Google Scholar]
  15. Kämpfer P., Martin E., Lodders N., Jäckel U., Huber B. E., Schumann P., Langer S., Busse H. J., Scholz H.. ( 2010;). Paenochrobactrum gallinarii gen. nov., sp. nov., isolated from air of a duck barn, and reclassification of Pseudochrobactrum glaciei as Paenochrobactrum glaciei comb. nov.. Int J Syst Evol Microbiol 60:, 1493–1498. [CrossRef][PubMed]
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  17. Pitcher D. G., Saunders N. A., Owen R. J.. ( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. . Lett Appl Microbiol 8:, 151–156. [CrossRef]
    [Google Scholar]
  18. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28:, 1823–1829. [CrossRef][PubMed]
    [Google Scholar]
  19. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V.. ( 2008;). Pseudochrobactrum glaciei sp. nov., isolated from sea ice collected from Peter the Great Bay of the Sea of Japan. . Int J Syst Evol Microbiol 58:, 2454–2458. [CrossRef][PubMed]
    [Google Scholar]
  20. Scholz H. C., Tomaso H., Al Dahouk S., Witte A., Schloter M., Kämpfer P., Falsen E., Neubauer H.. ( 2006;). Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. . FEMS Microbiol Lett 257:, 7–16. [CrossRef][PubMed]
    [Google Scholar]
  21. Scholz H. C., Al Dahouk S., Tomaso H., Neubauer H., Witte A., Schloter M., Kämpfer P., Falsen E., Pfeffer M., Engel M.. ( 2008;). Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum–Brucella group by recA and 16S rRNA gene-based comparative sequence analysis. . Syst Appl Microbiol 31:, 1–16. [CrossRef][PubMed]
    [Google Scholar]
  22. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22:, 2688–2690. [CrossRef][PubMed]
    [Google Scholar]
  23. Stolz A., Busse H. J., Kämpfer P.. ( 2007;). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:, 572–576. [CrossRef][PubMed]
    [Google Scholar]
  24. Tindall B. J.. ( 1990a;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  25. Tindall B. J.. ( 1990b;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  26. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  27. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31:, 241–250. [CrossRef][PubMed]
    [Google Scholar]
  28. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R.. ( 1998;). Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48:, 179–186. [CrossRef][PubMed]
    [Google Scholar]
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