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Abstract

A Gram-stain-positive, aerobic, non-flagellated bacterium, designated KIS22-12, was isolated from a soil sample of Baengnyeong Island in Onjin county, Republic of Korea. Cells were non-spore-forming cocci showing catalase-positive and oxidase-negative reactions. Growth of strain KIS22-12 was observed between 10 and 35 °C (optimum, 28–30 °C), between pH 5.0 and 9.0 (optimum, pH 7.0) and with 0–3 % (w/v) NaCl. Strain KIS22-12 contained MK-8(H) as the predominant menaquinone, and Cω8, iso-C and anteiso-C as the major fatty acids. Strain KIS22-12 contained diphosphatidylglycerol, phosphatidylinositol, one unknown aminophospholipid, one unknown aminolipid, two unknown phospholipids and one unknown lipid. The peptidoglycan type was A1γ. The G+C content of the genomic DNA was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KIS22-12 formed a phyletic lineage with KV-628. 16S rRNA gene sequence similarity between the two strains was 96.5 %. On the basis of phenotypic, chemotaxonomic and molecular properties, strain KIS22-12 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is KIS22-12 ( = KACC 16543 = DSM 27161 = NBRC 109596). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • , Research Program for Agricultural Science & Technology Development, National Academy of Agricultural Science, Rural Development Administration, Republic of Korea , (Award  PJ008666)
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2014-07-01
2020-11-30
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