1887

Abstract

A facultatively alkaliphilic, lactic-acid-producing and halophilic strain, designated SG103, was isolated from a fermented indigo ( Lour.) liquor sample for dyeing prepared in a laboratory. 16S rRNA gene sequence phylogeny suggested that SG103 is a member of the genus with the closest relatives being ‘ ’ J2 (similarity: 97.06 %), TP2-8 (97.06 %) and NN (96.87 %). Cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. The strain grew at temperatures between 13 and 48 °C with the optimum at 39 °C. It grew in the range pH 7–10 with the optimum at pH 9. The isoprenoid quinone detected was menaquinone-7 (MK-7) and the DNA G+C content was 41.3 mol%. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C, anteiso-C and anteiso-C. Unlike other reported species of the genus , the strain lacked diphosphatidylglycerol as a major polar lipid. DNA–DNA hybridization experiments with strains exhibiting greater than 96.87 % 16S rRNA gene sequence similarity, ‘. ’ J2, TP2-8 and NN, revealed 2±4 %, 4±9 % and 3±2 % relatedness, respectively. On the basis of the differences in phenotypic and chemotaxonomic characteristics, and the results of phylogenetic analyses based on 16S rRNA gene sequences and DNA–DNA relatedness data from reported species of the genus , strain SG103 merits classification as a members of a novel species, for which the name sp. nov. is proposed. The type strain is SG103 ( = JCM 17253 = NCIMB 14683).

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2014-09-01
2019-11-13
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References

  1. Ahmed I. , Yokota A. , Fujiwara T. . ( 2007; ). Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. . Int J Syst Evol Microbiol 57:, 796–802. [CrossRef] [PubMed]
    [Google Scholar]
  2. Aino K. , Narihiro T. , Minamida K. , Kamagata Y. , Yoshimune K. , Yumoto I. . ( 2010; ). Bacterial community characterization and dynamics of indigo fermentation. . FEMS Microbiol Ecol 74:, 174–183. [CrossRef] [PubMed]
    [Google Scholar]
  3. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  4. Carrasco I. J. , Márquez M. C. , Yanfen X. , Ma Y. , Cowan D. A. , Jones B. E. , Grant W. D. , Ventosa A. . ( 2006; ). Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 56:, 599–604. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chamroensaksri N. , Tanasupawat S. , Akaracharanya A. , Visessanguan W. , Kudo T. , Itoh T. . ( 2010; ). Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra). . Int J Syst Evol Microbiol 60:, 944–948. [CrossRef] [PubMed]
    [Google Scholar]
  6. Chen Y. G. , Cui X. L. , Zhang Y. Q. , Li W. J. , Wang Y. X. , Xu L. H. , Peng Q. , Wen M. L. , Jiang C. L. . ( 2008a; ). Gracilibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from saline soil. . Int J Syst Evol Microbiol 58:, 2403–2408. [CrossRef] [PubMed]
    [Google Scholar]
  7. Chen Y. G. , Cui X. L. , Zhang Y. Q. , Li W. J. , Wang Y. X. , Xu L. H. , Peng Q. , Wen M. L. , Jiang C. L. . ( 2008b; ). Gracilibacillus quinghaiensis sp. nov., isolated from salt-lake sediment in the Qaidam Basin, north-west China. . Syst Appl Microbiol 31:, 183–189. [CrossRef] [PubMed]
    [Google Scholar]
  8. Clejan S. , Krulwich T. A. , Mondrus K. R. , Seto-Young D. . ( 1986; ). Membrane lipid composition of obligately and facultatively alkalophilic strains of Bacillus spp.. J Bacteriol 168:, 334–340.[PubMed]
    [Google Scholar]
  9. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acids. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  10. Enomoto K. , Koyama N. . ( 1999; ). Effect of growth pH on the phospholipid contents of the membranes from alkaliphilic bacteria. . Curr Microbiol 39:, 270–273. [CrossRef] [PubMed]
    [Google Scholar]
  11. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  12. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  13. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  14. Gao M. , Liu Z. Z. , Zhou Y. G. , Liu H. C. , Ma Y. C. , Wang L. , Chen S. F. , Ji X. C. . ( 2012; ). Gracilibacillus kekensis sp. nov., a moderate halophile isolated from Keke Salt Lake. . Int J Syst Evol Microbiol 62:, 1032–1036. [CrossRef] [PubMed]
    [Google Scholar]
  15. Guindon S. , Gascuel O. . ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef] [PubMed]
    [Google Scholar]
  16. Hirota K. , Hanaoka Y. , Nodasaka Y. , Yumoto I. . ( 2013; ). Oceanobacillus polygoni sp. nov., a facultatively alkaliphile isolated from indigo fermentation fluid. . Int J Syst Evol Microbiol 63:, 3307–3312. [CrossRef] [PubMed]
    [Google Scholar]
  17. Huang H. Q. , Wang Y. , Yuan W. D. , Xiao C. , Ye J. J. , Liu M. , Zhu J. , Sun Q. G. , Bao S. X. . ( 2013; ). Gracilibacillus marinus sp. nov., isolated from the northern South China Sea. . Antonie van Leeuwenhoek 104:, 695–701. [CrossRef] [PubMed]
    [Google Scholar]
  18. Huo Y. Y. , Xu X. W. , Cui H. L. , Wu M. . ( 2010; ). Gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil. . Int J Syst Evol Microbiol 60:, 1383–1386. [CrossRef] [PubMed]
    [Google Scholar]
  19. Jeon C. O. , Lim J. M. , Jang H. H. , Park D. J. , Xu L. H. , Jiang C. L. , Kim C. J. . ( 2008; ). Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China. . Int J Syst Evol Microbiol 58:, 2282–2286. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kim P. , Lee J. C. , Park D. J. , Shin K. S. , Kim J. Y. , Kim C. J. . ( 2012; ). Gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from solar saltern soil. . Int J Syst Evol Microbiol 62:, 1857–1863. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120.[CrossRef]
    [Google Scholar]
  22. Lawson P. A. , Deutch C. E. , Collins M. D. . ( 1996; ). Phylogenetic characterization of a novel salt-tolerant Bacillus species: description of Bacillus dipsosauri sp. nov.. J Appl Bacteriol 81:, 109–112. [CrossRef] [PubMed]
    [Google Scholar]
  23. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  24. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  25. Nakajima K. , Hirota K. , Nodasaka Y. , Yumoto I. . ( 2005; ). Alkalibacterium iburiense sp. nov., an obligate alkaliphile that reduces an indigo dye. . Int J Syst Evol Microbiol 55:, 1525–1530. [CrossRef] [PubMed]
    [Google Scholar]
  26. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  27. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  28. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  29. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  30. Tang S. K. , Wang Y. , Lou K. , Mao P. H. , Jin X. , Jiang C. L. , Xu L. H. , Li W. J. . ( 2009; ). Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 59:, 1620–1624. [CrossRef] [PubMed]
    [Google Scholar]
  31. Teather R. M. , Wood P. J. . ( 1982; ). Use of Congo red polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol 43:, 777–780.[PubMed]
    [Google Scholar]
  32. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  33. Wainø M. , Tindall B. J. , Schumann P. , Ingvorsen K. . ( 1999; ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. Int J Syst Bacteriol 49:, 821–831. [CrossRef] [PubMed]
    [Google Scholar]
  34. Yang N. , Ren B. , Dai H. , Liu Z. , Zhou Y. , Song F. , Zhang L. . ( 2013; ). Gracilibacillus xinjiangensis sp. nov., a new member of the genus Gracilibacillus isolated from Xinjiang region, China. . Antonie van Leeuwenhoek 104:, 809–816. [CrossRef] [PubMed]
    [Google Scholar]
  35. Yumoto I. , Yamazaki K. , Sawabe T. , Nakano K. , Kawasaki K. , Ezura Y. , Shinano H. . ( 1998; ). Bacillus horti sp. nov., a new gram-negative alkaliphilic bacillus. . Int J Syst Bacteriol 48:, 565–571. [CrossRef] [PubMed]
    [Google Scholar]
  36. Yumoto I. , Yamazaki K. , Hishinuma M. , Nodasaka Y. , Suemori A. , Nakajima K. , Inoue N. , Kawasaki K. . ( 2001; ). Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. . Int J Syst Evol Microbiol 51:, 349–355.[PubMed]
    [Google Scholar]
  37. Yumoto I. , Nakamura A. , Iwata H. , Kojima K. , Kusumoto K. , Nodasaka Y. , Matsuyama H. . ( 2002; ). Dietzia psychralcaliphila sp. nov., a novel, facultatively psychrophilic alkaliphile that grows on hydrocarbons. . Int J Syst Evol Microbiol 52:, 85–90.[PubMed]
    [Google Scholar]
  38. Yumoto I. , Hirota K. , Nodasaka Y. , Yokota Y. , Hoshino T. , Nakajima K. . ( 2004; ). Alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye. . Int J Syst Evol Microbiol 54:, 2379–2383. [CrossRef] [PubMed]
    [Google Scholar]
  39. Yumoto I. , Hirota K. , Nodasaka Y. , Tokiwa Y. , Nakajima K. . ( 2008; ). Alkalibacterium indicireducens sp. nov., an obligate alkaliphile that reduces indigo dye. . Int J Syst Evol Microbiol 58:, 901–905. [CrossRef] [PubMed]
    [Google Scholar]
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