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Volume 64, Issue Pt_9

sp. nov., a phototrophic alphaproteobacterium isolated from a mangrove forest sediment sample Free

Abstract

A novel Gram-staining-negative, purple non-sulfur bacterium, strain AK41, was isolated from a sediment sample collected from Coringa mangrove forest, Andhra Pradesh, India. A red–brownish-coloured culture was obtained on modified Pfennig medium after enrichment with 2 % NaCl and 0.3 % pyruvate under 2000 lx illumination. Individual cells were ovoid–rod-shaped and non-motile. Bacteriochlorophyll and carotenoids of the spheroidene series were present as photosynthetic pigments. Strain AK41 was halophilic and grew photoheterotrophically with a number of organic compounds as carbon sources and electron donors. It was unable to grow photoautotrophically. It did not utilize sulfide or thiosulfate as electron donors. The fatty acids were found to be dominated by C and Cω7. Strain AK41 contained phosphatidylglycerol, phosphatidylethanolamine, an unknown aminolipid and four unknown lipids as polar lipids. Q-10 was the predominant respiratory quinone. The DNA G+C content of strain AK41 was 68.9 mol%. 16S rRNA gene sequence analysis indicated that strain AK41 was a member of the genus and was closely related to , with 96.0 % similarity to the type strain; the 16S rRNA gene sequence similarity to the type strains of other species of the genus was 93.9–95.8 %. Phylogenetic analyses indicated that strain AK41 clustered with the type strains of , , and with sequence similarity of 95.9–96.2 %. Based on data from the current study, strain AK41 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is AK41 ( = MTCC 11825 = JCM 19220).

  • Published Online:
Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR)
  • IMTECH, Chandigarh
  • NIO, Goa
  • Department of Biotechnology, Government of India
© 2014 IUMS
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2014-09-01
2024-04-26
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Rhodovulummangrovi sp. nov., a phototrophic alphaproteobacterium isolated from a mangrove forest sediment sample
Int J Syst Evol Microbiol 64, 3168 (2014); https://doi.org/10.1099/ijs.0.059857-0
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