1887

Abstract

A Gram-staining-negative, strictly aerobic, motile (by gliding), non-spore-forming and rod-shaped bacterial strain, designated THG-DT86, was isolated from soil of a ginseng field of Pocheon province in the Republic of Korea and its taxonomic position was investigated by a polyphasic approach. Growth occurred at 10–35 °C, at pH 6.5–8.5 and with 0–1.5 % (w/v) NaCl on trypticase soy agar. Flexirubin-type pigments were found to be present. On the basis of 16S rRNA gene sequence similarity, strain THG-DT86 was shown to belong to the genus and was related to PTJT-5 (95.3 %). The G+C content of the genomic DNA was 50.1 mol%. The only isoprenoid quinone detected in strain THG-DT86 was menaquinone-7 (MK-7) and the only polyamine was homospermidine. The predominant fatty acids were iso-C, iso-C 3-OH, C, iso-C G and iso-C, and the major polar lipids were phosphatidylethanolamine, an unidentified aminophosphoglycolipid and an unidentified aminophospholipid. Phenotypic data and phylogenetic inference supported the affiliation of strain THG-DT86 to the genus , and a number of biochemical tests differentiated strain THG-DT86 from the recognized species of the genus . Therefore, the novel isolate represents a novel species, for which the name sp. nov. is proposed, with THG-DT86 as the type strain ( = KACC 17171 = JCM 18823).

Funding
This study was supported by the:
  • Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Award 810006-03-1-CG100)
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2014-03-01
2021-10-18
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