1887

Abstract

A halophilic archaeal strain, GX48, was isolated from the Gangxi marine solar saltern near Weihai city in Shandong Province, China. Cells of the strain were rod-shaped, stained Gram-negative and formed red-pigmented colonies. Strain GX48 was able to grow at 25–50 °C (optimum 37 °C), in the presence of 1.4–4.8 M NaCl (optimum 2.6 M NaCl), with 0–1.0 M MgCl (optimum 0.05 M MgCl) and at pH 5.5–9.5 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to those of YSM-123 and 294-194-5. 16S rRNA gene analysis revealed that strain GX48 had two dissimilar 16S rRNA genes and both of them were phylogenetically related to those of the two current members of the genus (96.2–98.3 % similarities). The gene sequence similarities between strain GX48 and YSM-123 and 294-194-5 were 96.0 % and 94.7 %, respectively. The DNA G+C content of strain GX48 was 66.2 mol%. Strain GX48 showed low DNA–DNA relatedness with the two members of the genus . It was concluded that strain GX48 ( = CGMCC 1.10388 = JCM 17119) represents a novel species of the genus , for which the name sp. nov. is proposed. An emended description of the genus is also presented.

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2014-03-01
2019-12-11
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References

  1. Cui H.-L. , Zhou P.-J. , Oren A. , Liu S.-J. . ( 2009; ). Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium . . Extremophiles 13:, 31–37. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cui H.-L. , Gao X. , Yang X. , Xu X.-W. . ( 2010; ). Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. . Extremophiles 14:, 493–499. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cui H.-L. , Yang X. , Mou Y. Z. . ( 2011; ). Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria . . Extremophiles 15:, 625–631. [CrossRef] [PubMed]
    [Google Scholar]
  4. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  5. Echigo A. , Minegishi H. , Shimane Y. , Kamekura M. , Itoh T. , Usami R. . ( 2013; ). Halomicroarcula pellucida gen. nov., sp. nov., a non-pigmented, transparent-colony-forming, halophilic archaeon isolated from solar salt. . Int J Syst Evol Microbiol 63:, 3556–3562. [CrossRef] [PubMed]
    [Google Scholar]
  6. Han D. , Cui H.-L. . ( 2014; ). Halosimplex pelagicum sp. nov. and Halosimplex rubrum sp. nov., isolated from salted brown alga Laminaria, and emended description of the genus Halosimplex . . Int J Syst Evol Microbiol 64:, 169–173.[PubMed] [CrossRef]
    [Google Scholar]
  7. Huß V. A. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef] [PubMed]
    [Google Scholar]
  8. Liu B.-B. , Tang S.-K. , Cui H.-L. , Zhang Y.-G. , Li L. , Zhang Y.-M. , Zhang L.-L. , Li W.-J. . ( 2013; ). Halopelagius fulvigenes sp. nov., a halophilic archaeon isolated from a lake. . Int J Syst Evol Microbiol 63:, 2192–2196. [CrossRef] [PubMed]
    [Google Scholar]
  9. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  10. Minegishi H. , Echigo A. , Nagaoka S. , Kamekura M. , Usami R. . ( 2010a; ). Halarchaeum acidiphilum gen. nov., sp. nov., a moderately acidophilic haloarchaeon isolated from commercial solar salt. . Int J Syst Evol Microbiol 60:, 2513–2516. [CrossRef] [PubMed]
    [Google Scholar]
  11. Minegishi H. , Kamekura M. , Itoh T. , Echigo A. , Usami R. , Hashimoto T. . ( 2010b; ). Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B′ (rpoB′) gene. . Int J Syst Evol Microbiol 60:, 2398–2408. [CrossRef] [PubMed]
    [Google Scholar]
  12. Minegishi H. , Yamauchi Y. , Echigo A. , Shimane Y. , Kamekura M. , Itoh T. , Ohkuma M. , Usami R. . ( 2013; ). Halarchaeum nitratireducens sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt. . Int J Syst Evol Microbiol 63:, 4202–4206. [CrossRef] [PubMed]
    [Google Scholar]
  13. Nagaoka S. , Minegishi H. , Echigo A. , Usami R. . ( 2010; ). Halostagnicola kamekurae sp. nov., an extremely halophilic archaeon from solar salt. . Int J Syst Evol Microbiol 60:, 2828–2831. [CrossRef] [PubMed]
    [Google Scholar]
  14. Nagaoka S. , Minegishi H. , Echigo A. , Shimane Y. , Kamekura M. , Usami R. . ( 2011; ). Halostagnicola alkaliphila sp. nov., an alkaliphilic haloarchaeon from commercial rock salt. . Int J Syst Evol Microbiol 61:, 1149–1152. [CrossRef] [PubMed]
    [Google Scholar]
  15. Oren A. . ( 2012; ). Taxonomy of the family Halobacteriaceae: a paradigm for changing concepts in prokaryote systematics. . Int J Syst Evol Microbiol 62:, 263–271. [CrossRef] [PubMed]
    [Google Scholar]
  16. Oren A. , Ventosa A. , Grant W. D. . ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales . . Int J Syst Bacteriol 47:, 233–238. [CrossRef]
    [Google Scholar]
  17. Qiu X.-X. , Mou Y.-Z. , Zhao M.-L. , Zhang W.-J. , Han D. , Ren M. , Cui H.-L. . ( 2013; ). Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China. . Int J Syst Evol Microbiol 63:, 3975–3980. [CrossRef] [PubMed]
    [Google Scholar]
  18. Shimane Y. , Hatada Y. , Minegishi H. , Mizuki T. , Echigo A. , Miyazaki M. , Ohta Y. , Usami R. , Grant W. D. , Horikoshi K. . ( 2010; ). Natronoarchaeum mannanilyticum gen. nov., sp. nov., an aerobic, extremely halophilic archaeon isolated from commercial salt. . Int J Syst Evol Microbiol 60:, 2529–2534. [CrossRef] [PubMed]
    [Google Scholar]
  19. Shimane Y. , Hatada Y. , Minegishi H. , Echigo A. , Nagaoka S. , Miyazaki M. , Ohta Y. , Maruyama T. , Usami R. . & other authors ( 2011; ). Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt. . Int J Syst Evol Microbiol 61:, 2266–2270. [CrossRef] [PubMed]
    [Google Scholar]
  20. Shimane Y. , Nagaoka S. , Minegishi H. , Kamekura M. , Echigo A. , Hatada Y. , Ito T. , Usami R. . ( 2013; ). Natronoarchaeum philippinense sp. nov., a haloarchaeon isolated from commercial solar salt. . Int J Syst Evol Microbiol 63:, 920–924. [CrossRef] [PubMed]
    [Google Scholar]
  21. Shimoshige H. , Yamada T. , Minegishi H. , Echigo A. , Shimane Y. , Kamekura M. , Itoh T. , Usami R. . ( 2013; ). Halobaculum magnesiiphilum sp. nov., a magnesium-dependent haloarchaeon isolated from commercial salt. . Int J Syst Evol Microbiol 63:, 861–866. [CrossRef] [PubMed]
    [Google Scholar]
  22. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  23. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  24. Wang S. , Yang Q. , Liu Z.-H. , Sun L. , Wei D. , Zhang J.-Z. , Song J.-Z. , Yuan H.-F. . ( 2010; ). Haloterrigena daqingensis sp. nov., an extremely haloalkaliphilic archaeon isolated from a saline–alkaline soil. . Int J Syst Evol Microbiol 60:, 2267–2271. [CrossRef] [PubMed]
    [Google Scholar]
  25. Yamauchi Y. , Minegishi H. , Echigo A. , Shimane Y. , Shimoshige H. , Kamekura M. , Itoh T. , Doukyu N. , Inoue A. , Usami R. . ( 2013a; ). Halarchaeum salinum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt. . Int J Syst Evol Microbiol 63:, 1138–1142. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yamauchi Y. , Minegishi H. , Echigo A. , Shimane Y. , Kamekura M. , Itoh T. , Ohkuma M. , Doukyu N. , Inoue A. , Usami R. . ( 2013b; ). Halarchaeum rubridurum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt samples. . Int J Syst Evol Microbiol 63:, 3143–3147. [CrossRef] [PubMed]
    [Google Scholar]
  27. Zhang X. , Zhang W.-Y. , Shen A.-H. , Huo Y.-Y. , Zhu X.-F. , Wu M. . ( 2013; ). Halopelagius longus sp. nov., a member of the family Halobacteriaceae isolated from a salt mine, and emended description of the genus Halopelagius . . Int J Syst Evol Microbiol 63:, 3585–3590. [CrossRef] [PubMed]
    [Google Scholar]
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