1887

Abstract

A rod-shaped, motile, facultatively anaerobic and moderately halotolerant plant-growth-promoting actinobacterial strain, designated NCCP-11, was isolated from paddy grains. To delineate its taxonomic position, the strain was subjected to a polyphasic characterization. Cells of strain NCCP-11 grew at 10–37 °C (optimum 28–32 °C), at pH 6–9 (optimum pH 7) and in 0–12 % (w/v) NaCl (optimum 1–2 %) in broth medium. Based on 16S rRNA gene sequence analysis, strain NCCP-11 showed highest similarity to the type strains of (98.99 %) and (98.09 %) and less than 97 % with other closely related taxa. The chemotaxonomic data [major menaquinone: MK-9(H4); cell-wall peptidoglycan: type A4β; major fatty acids: anteiso-C, C, C and anteiso-C; major polar lipids: diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannosides and two unknown polar lipids] also supported the affiliation of strain NCCP-11 to the genus The level of DNA–DNA relatedness between strain NCCP-11 and the two type strains mentioned above was less than 42.7 %. On the basis of DNA–DNA relatedness, physiological and biochemical characteristics and phylogenetic position, strain NCCP-11 can be differentiated from species of the genus with validly published names and thus represents a novel species, for which the name sp. nov. is proposed. The type strain is NCCP-11 ( = DSM 24792 = JCM 18755 = KCTC 19798).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.059063-0
2014-07-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2305.html?itemId=/content/journal/ijsem/10.1099/ijs.0.059063-0&mimeType=html&fmt=ahah

References

  1. Ahmed I., Yokota A., Fujiwara T.. ( 2007;). A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth. . Extremophiles 11:, 217–224. [CrossRef][PubMed]
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M.. ( 1923;). Bergey’s Manual of Determinative Bacteriology. Baltimore:: Williams &Wilkins;.
    [Google Scholar]
  3. Brown J. M., Frazier R. P., Morey R. E., Steigerwalt A. G., Pellegrini G. J., Daneshvar M. I., Hollis D. G., McNeil M. M.. ( 2005;). Phenotypic and genetic characterization of clinical isolates of CDC coryneform group A-3: proposal of a new species of Cellulomonas, Cellulomonas denverensis sp. nov.. J Clin Microbiol 43:, 1732–1737. [CrossRef][PubMed]
    [Google Scholar]
  4. Euzéby J. P.. ( 2014;). List of prokaryotic names with standing in nomenclature: a folder available on the Internet. . [Last full update May 14, 2014]. (http://www.bacterio.net/cellulomonas.html).
  5. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. ( 2005;). phylip (phylogeny inference package) version 3.6. . Distributed by the author. Department of Genome Sciences, University of Washington;: Seattle, USA:.
  7. Funke G., Ramos C. P., Collins M. D.. ( 1995;). Identification of some clinical strains of CDC coryneform group A-3 and A-4 bacteria as Cellulomonas species and proposal of Cellulomonas hominis sp. nov. for some group A-3 strains. . J Clin Microbiol 33:, 2091–2097.[PubMed]
    [Google Scholar]
  8. Hatayama K., Esaki K., Ide T.. ( 2013;). Cellulomonas soli sp. nov. and Cellulomonas oligotrophica sp. nov., isolated from soil. . Int J Syst Evol Microbiol 63:, 60–65. [CrossRef][PubMed]
    [Google Scholar]
  9. Jones B. E., Grant W. D., Duckworth A. W., Schumann P., Weiss N., Stackebrandt E.. ( 2005;). Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad. . Int J Syst Evol Microbiol 55:, 1711–1714. [CrossRef][PubMed]
    [Google Scholar]
  10. Kang M.-S., Im W.-T., Jung H.-M., Kim M. K., Goodfellow M., Kim K. K., Yang H.-C., An D.-S., Lee S.-T.. ( 2007;). Cellulomonas composti sp. nov., a cellulolytic bacterium isolated from cattle farm compost. . Int J Syst Evol Microbiol 57:, 1256–1260. [CrossRef][PubMed]
    [Google Scholar]
  11. Kawamoto I., Oka T., Nara T.. ( 1981;). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. . J Bacteriol 146:, 527–534.[PubMed]
    [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  13. Kudo T.. ( 2001;). Phospholipids. . In Identification Manual of Bacteria: Molecular Genetics and Molecular Biological Methods, pp. 135–144. Edited by Suzuki K., Hiraishi A., Yokota A... Tokyo:: Springer; [in Japanese].
    [Google Scholar]
  14. Lee C.-M., Weon H.-Y., Hong S.-B., Jeon Y.-A., Schumann P., Kroppenstedt R. M., Kwon S.-W., Stackebrandt E.. ( 2008;). Cellulomonas aerilata sp. nov., isolated from an air sample. . Int J Syst Evol Microbiol 58:, 2925–2929. [CrossRef][PubMed]
    [Google Scholar]
  15. Mandel M., Igambi L., Bergendahl J., Dodson M. L. Jr, Scheltgen E.. ( 1970;). Correlation of melting temperature and caesium chloride buoyant density of bacterial deoxyribonucleic acid. . J Bacteriol 101:, 333–338.[PubMed]
    [Google Scholar]
  16. Mikami H., Ishida Y.. ( 1983;). Post-column fluorometric detection of reducing sugars in high performance liquid chromatography using arginine. . Bunseki Kagaku 32:, E207–E210. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Rivas R., Trujillo M. E., Mateos P. F., Martínez-Molina E., Velázquez E.. ( 2004;). Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree. . Int J Syst Evol Microbiol 54:, 533–536. [CrossRef][PubMed]
    [Google Scholar]
  19. Rusznyák A., Tóth E. M., Schumann P., Spröer C., Makk J., Szabó G., Vladár P., Márialigeti K., Borsodi A. K.. ( 2011;). Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond. . Int J Syst Evol Microbiol 61:, 1662–1666. [CrossRef][PubMed]
    [Google Scholar]
  20. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  21. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  22. Shi Z., Luo G., Wang G.. ( 2012;). Cellulomonas carbonis sp. nov., isolated from coal mine soil. . Int J Syst Evol Microbiol 62:, 2004–2010. [CrossRef][PubMed]
    [Google Scholar]
  23. Stackebrandt E., Schumann P.. ( 2012;). Genus I. Cellulomonas, Bergey, Harrison, Breed, Hammer and Huntoon 1923, 154 emend. mut. char. Clark 1952, 50 AL.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 5, pp. 702–710. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-i., Ludwig W., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  24. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  28. Zhang L., Xi L., Qiu D., Song L., Dai X., Ruan J., Huang Y.. ( 2013;). Cellulomonas marina sp. nov., isolated from deep-sea water. . Int J Syst Evol Microbiol 63:, 3014–3018. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.059063-0
Loading
/content/journal/ijsem/10.1099/ijs.0.059063-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error