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Abstract

A rod-shaped, motile, facultatively anaerobic and moderately halotolerant plant-growth-promoting actinobacterial strain, designated NCCP-11, was isolated from paddy grains. To delineate its taxonomic position, the strain was subjected to a polyphasic characterization. Cells of strain NCCP-11 grew at 10–37 °C (optimum 28–32 °C), at pH 6–9 (optimum pH 7) and in 0–12 % (w/v) NaCl (optimum 1–2 %) in broth medium. Based on 16S rRNA gene sequence analysis, strain NCCP-11 showed highest similarity to the type strains of (98.99 %) and (98.09 %) and less than 97 % with other closely related taxa. The chemotaxonomic data [major menaquinone: MK-9(H4); cell-wall peptidoglycan: type A4β; major fatty acids: anteiso-C, C, C and anteiso-C; major polar lipids: diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannosides and two unknown polar lipids] also supported the affiliation of strain NCCP-11 to the genus The level of DNA–DNA relatedness between strain NCCP-11 and the two type strains mentioned above was less than 42.7 %. On the basis of DNA–DNA relatedness, physiological and biochemical characteristics and phylogenetic position, strain NCCP-11 can be differentiated from species of the genus and thus represents a novel species, for which the name sp. nov. is proposed. The type strain is NCCP-11 ( = DSM 24792 = JCM 18755 = KCTC 19798).

Funding
This study was supported by the:
  • , Public Sector Development Programme (PSDP) , (Award CS-55/RADP/PARC)
  • , Pakistan Agricultural Research Council (PARC)
  • , Japan Society for Promotion of Science (JSPS)
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2014-07-01
2020-08-13
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