1887

Abstract

A Gram-staining-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK3-3, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK3-3 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK3-3 clustered with the type strains of species of the genus , with which it exhibited 96.5–97.2 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of other recognized species were less than 92.2 %. Strain SSK3-3 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The major polar lipids of strain SSK3-3 were phosphatidylethanolamine, one unidentified lipid and one unidentified glycolipid. The DNA G+C content of strain SSK3-3 was 34.9 mol% and the mean DNA–DNA relatedness with KACC 14311 was 12 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK3-3 is separate from other species of the genus . On the basis of the data presented, strain SSK3-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SSK3-3 ( = KCTC 32426 = CECT 8368). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • , National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE)
  • , Program for Collection, Management and Utilization of Biological Resources and BK 21 program from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
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2014-02-01
2021-01-19
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