Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_2

sp. nov., isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Free

Abstract

A Gram-staining-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK3-3, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK3-3 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK3-3 clustered with the type strains of species of the genus , with which it exhibited 96.5–97.2 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of other recognized species were less than 92.2 %. Strain SSK3-3 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The major polar lipids of strain SSK3-3 were phosphatidylethanolamine, one unidentified lipid and one unidentified glycolipid. The DNA G+C content of strain SSK3-3 was 34.9 mol% and the mean DNA–DNA relatedness with KACC 14311 was 12 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK3-3 is separate from other species of the genus . On the basis of the data presented, strain SSK3-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SSK3-3 ( = KCTC 32426 = CECT 8368). An emended description of the genus is also provided.

  • Published Online:
Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE)
  • Program for Collection, Management and Utilization of Biological Resources and BK 21 program from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058776-0
2014-02-01
2025-05-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/2/657.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058776-0&mimeType=html&fmt=ahah

References

  1. Baek K., Choi A., Kang I., Lee K., Cho J.-C. ( 2013 ). Kordia antarctica sp. nov., isolated from Antarctic seawater. . Int J Syst Evol Microbiol 63, 3617–3622. [View Article] [PubMed]
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  3. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
     [Google Scholar]
  4. Bernardet J.-F. ( 2011 ). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. . New York:: Springer;.
     [Google Scholar]
  5. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  6. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  7. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 1997–2006. [View Article] [PubMed]
    [Google Scholar]
  8. Choi A., Oh H.-M., Yang S.-J., Cho J.-C. ( 2011 ). Kordia periserrulae sp. nov., isolated from a marine polychaete Periserrula leucophryna, and emended description of the genus Kordia . . Int J Syst Evol Microbiol 61, 864–869. [View Article] [PubMed]
    [Google Scholar]
  9. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 25–68. [View Article]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  11. Komagata K., Suzuki K. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  12. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 1–67. [View Article]
    [Google Scholar]
  13. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104–117. [View Article]
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  15. Reichenbach H. ( 1992 ). The order Cytophagales . . In The Prokaryotes, , 2nd edn., pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  16. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  17. Sohn J. H., Lee J.-H., Yi H., Chun J., Bae K. S., Ahn T.-Y., Kim S.-J. ( 2004 ). Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide. . Int J Syst Evol Microbiol 54, 675–680. [View Article] [PubMed]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  19. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 1921–1942.[PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  22. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502–505. [View Article]
    [Google Scholar]
  23. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187–194. [View Article] [PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. ( 2003 ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 53–57. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.058776-0
Loading
Kordia jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Kordia
Int J Syst Evol Microbiol 64, 657 (2014); https://doi.org/10.1099/ijs.0.058776-0
/content/journal/ijsem/10.1099/ijs.0.058776-0
/content/journal/ijsem/10.1099/ijs.0.058776-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error