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Abstract

An extremely halophilic archaeon, strain D108, was isolated from a brine sample of Aran-Bidgol salt lake in Iran. The novel strain was cream-pigmented, motile, pleomorphic rod-shaped and required at least 2.5 M NaCl but not MgCl for growth. Optimal growth was achieved with 4.3 M NaCl and 0.1 M MgCl. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, and the strain was able to grow over a pH range of 6.5 to 9.0, and a temperature range of 30 to 50 °C. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain D108 clustered with the type strain of the sole species of the genus , TNN28, with a sequence similarity of 98.8 %. The polar lipid pattern of strain D108 consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, one phosphoglycolipid and two glycolipids. The only quinone present was MK-8(II-H). The DNA GC content of strain D108 was 62.8 mol%. DNA–DNA hybridization studies (45 % with IBRC-M 10760), as well as biochemical and physiological characterization, allowed strain D108 to be differentiated from . A novel species of the genus , sp. nov., is therefore proposed to accommodate this strain. The type strain is D108 ( = IBRC-M 10043 = CECT 8375). An emended description of the genus is also proposed.

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2014-03-01
2021-10-25
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References

  1. Balch W. E., Wolfe R. S. ( 1976 ). New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized [sic] atmosphere. . Appl Environ Microbiol 32, 781791.[PubMed]
    [Google Scholar]
  2. Bryant M. P. ( 1972 ). Commentary on the Hungate technique for culture of anaerobic bacteria. . Am J Clin Nutr 25, 13241328.[PubMed]
    [Google Scholar]
  3. Cui H. L., Yang X., Gao X., Xu X. W. ( 2011 ). Halobellus clavatus gen. nov., sp. nov. and Halorientalis regularis gen. nov., sp. nov., two new members of the family Halobacteriaceae . . Int J Syst Evol Microbiol 61, 26822689. [View Article] [PubMed]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  5. DeLong E. F. ( 1992 ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89, 56855689. [View Article] [PubMed]
    [Google Scholar]
  6. Dussault H. P. ( 1955 ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70, 484485.[PubMed]
    [Google Scholar]
  7. Dyall-Smith M. ( 2008 ). The Halohandbook: Protocols for Haloarchaeal Genetics. . http://www.haloarchaea.com/resources/halohandbook.
    [Google Scholar]
  8. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  10. González C., Gutiérrez C., Ramirez C. ( 1978 ). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. . Can J Microbiol 24, 710715. [View Article] [PubMed]
    [Google Scholar]
  11. Gutiérrez C., González C. ( 1972 ). Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. . Appl Microbiol 24, 516517.[PubMed]
    [Google Scholar]
  12. Hezayen F. F., Rehm B. H. A., Tindall B. J., Steinbüchel A. ( 2001 ). Transfer of Natrialba asiatica B1T to Natrialba taiwanensis sp. nov. and description of Natrialba aegyptiaca sp. nov., a novel extremely halophilic, aerobic, non-pigmented member of the Archaea from Egypt that produces extracellular poly(glutamic acid). . Int J Syst Evol Microbiol 51, 11331142. [View Article] [PubMed]
    [Google Scholar]
  13. Huß V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  14. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. ( 1985 ). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. . Proc Natl Acad Sci U S A 82, 69556959. [View Article] [PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  16. Oren A., Ventosa A., Grant W. D. ( 1997 ). Proposed minimal standards for description of new taxa in the order Halobacteriales . . Int J Syst Bacteriol 47, 233238. [View Article]
    [Google Scholar]
  17. Rzhetsky A., Nei M. ( 1992 ). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9, 945967.
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  22. Wainø M., Tindall B. J., Ingvorsen K. ( 2000 ). Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. . Int J Syst Evol Microbiol 50, 183190. [View Article] [PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
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