1887

Abstract

Strain JC207 was isolated from a deep (265 m) sea sediment, and appeared as dark yellow colonies on agar plates with cells staining Gram-negative. Catalase, oxidase and caseinase were positive, while chitinase, gelatinase and amylase were negative. Major (>5 %) fatty acids were iso-C, anteiso-C, iso-Cω9, iso-C, iso-C 3-OH, iso-C 3-OH, iso-C and iso-CG. Strain JC207 contained phosphatidylethanolamine as the major polar lipid, with minor amounts of five unidentified lipids. A bacterial hopane derivative, diplopterol and adenosylhopane were the major hopanoids. Genomic DNA G+C content was 47.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC207 represented a member of the genus within the family of the phylum . Strain JC207 had sequence similarity with YIM-C338 (98 %), BH206 (97.6 %) and other members of the genus (<96.8 %). However, strain JC207 showed an average of 23.6±4 and 37±4 relatedness (based on DNA–DNA hybridization) with CGMCC 1.6308 ( = YIM-C338) and KCTC 12883 ( = BH206), respectively. Morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC207 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JC207 ( = KCTC 32444 = CGMCC 1.12641).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058149-0
2014-03-01
2020-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/3/984.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058149-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. Chakravarthy S. K., Ramaprasad E. V. V., Shobha E., Sasikala Ch., Ramana Ch. V.. ( 2012;). Rhodoplanes piscinae sp. nov. isolated from pond water. . Int J Syst Evol Microbiol 62:, 2828–2834. [CrossRef][PubMed]
    [Google Scholar]
  3. Chen Y. G., Cui X. L., Zhang Y.-Q., Li W. J., Wang Y. X., Kim C. J., Lim J.-M., Xu L.-H., Jiang C.-L.. ( 2008;). Salinimicrobium terrae sp. nov., isolated from saline soil, and emended description of the genus Salinimicrobium. . Int J Syst Evol Microbiol 58:, 2501–2504. [CrossRef][PubMed]
    [Google Scholar]
  4. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  5. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  6. Lee S. Y., Park S., Oh T. K., Yoon J. H.. ( 2012;). Salinimicrobium gaetbulicola sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 62:, 1027–1031. [CrossRef][PubMed]
    [Google Scholar]
  7. Lim J.-M., Jeon C. O., Lee S. S., Park D.-J., Xu L.-H., Jiang C.-L., Kim C.-J.. ( 2008;). Reclassification of Salegentibacter catena Ying et al. 2007 as Salinimicrobium catena gen. nov., comb. nov. and description of Salinimicrobium xinjiangense sp. nov., a halophilic bacterium isolated from Xinjiang province in China. . Int J Syst Evol Microbiol 58:, 438–442. [CrossRef][PubMed]
    [Google Scholar]
  8. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  9. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H. P.. ( 2013;). When should a DDH experiment be mandatory in microbial taxonomy. ? Arch Microbiol 195:, 413–418. [CrossRef][PubMed]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  11. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Han J., Zhukova N. V., Shevchenko L. S.. ( 2010;). Salinimicrobium marinum sp. nov., a halophilic bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Salinimicrobium and Salinimicrobium catena. . Int J Syst Evol Microbiol 60:, 2303–2306. [CrossRef][PubMed]
    [Google Scholar]
  12. Ramana V. V., Sasikala C., Ramaprasad E. V. V., Ramana C. V.. ( 2010;). Description of Ectothiorhodospira salini sp. nov.. J Gen Appl Microbiol 56:, 313–319. [CrossRef][PubMed]
    [Google Scholar]
  13. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Evol Microbiol 44:, 846–849. [CrossRef]
    [Google Scholar]
  14. Subhash Y., Sasikala Ch., Ramana Ch. V.. ( 2013a;). Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. . Int J Syst Evol Microbiol 63:, 3463–3469. [CrossRef][PubMed]
    [Google Scholar]
  15. Subhash Y., Tushar L., Sasikala Ch., Ramana ChV.. ( 2013b;). Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. . Int J Syst Evol Microbiol 63:, 4524–4532. [CrossRef][PubMed]
    [Google Scholar]
  16. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V.. ( 2013c;). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. . Int J Syst Evol Microbiol 63:, 2132–2137. [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  18. Trüper H. G., Pfennig N.. ( 1981;). Isolation of members of the families Chromatiaceae and Chlorobiaceae. . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279–289. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G... Berlin:: Springer;.
    [Google Scholar]
  19. Ying J.-Y., Liu Z.-P., Wang B.-J., Dai X., Yang S.-S., Liu S.-J.. ( 2007;). Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter. . Int J Syst Evol Microbiol 57:, 219–222. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.058149-0
Loading
/content/journal/ijsem/10.1099/ijs.0.058149-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error