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Abstract

A Gram-stain-negative, non-motile, rod- or coccoid-shaped bacterial strain, designated HD-22, belonging to the class , was isolated from a tidal flat sediment of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain HD-22 grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2–3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HD-22 fell within the clade comprising species of the genus , clustering with the type strains of and , with which it exhibited highest 16S rRNA gene sequence similarity (97.6–98.2 %). Levels of 16S rRNA gene sequence similarity between strain HD-22 and the type strains of the other species of the genus were in the range 94.4–97.5 %. The DNA G+C content was 64.6 mol% and mean DNA–DNA relatedness values between strain HD-22 and the type strains of and were 42.1, 40.1 and 27.0 %, respectively. Strain HD-22 contained Q-10 as the predominant ubiquinone and summed feature 8 (Cω7 and/or Cω6) as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain HD-22 is distinguishable from recognized species of the genus . On the basis of the data presented, strain HD-22 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HD-22 ( = KCTC 32179 = CCUG 63415).

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2014-03-01
2019-10-23
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