1887

Abstract

A polyphasic study was undertaken to determine the taxonomic position of 13 -like strains from humans ( = 8) and reptiles ( = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from analysis, 16S rRNA gene and sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA–DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to but clearly differentiated from recognized subspecies of . Therefore, this unique cluster of 13 strains represents a novel subspecies within the species , for which the name subsp. subsp. nov. is proposed, with strain 03-427 ( = ATCC BAA-2539 = LMG 27499) as the type strain. Although this novel taxon could not be differentiated from and using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish subsp. subsp. nov. from recognized subspecies of .

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2014-09-01
2021-10-21
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References

  1. Barrett T. J., Patton C. M., Morris G. K. ( 1988 ). Differentiation of Campylobacter species using phenotypic characterization. . Lab Med 19, 96102.
    [Google Scholar]
  2. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G. ( 1982 ). Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. . J Clin Microbiol 15, 11331140.[PubMed]
    [Google Scholar]
  3. Dingle K. E., Blaser M. J., Tu Z. C., Pruckler J., Fitzgerald C., van Bergen M. A., Lawson A. J., Owen R. J., Wagenaar J. A. ( 2010 ). Genetic relationships among reptilian and mammalian Campylobacter fetus strains determined by multilocus sequence typing. . J Clin Microbiol 48, 977980. [View Article] [PubMed]
    [Google Scholar]
  4. Gilbert M. J., Miller W. G., Yee E., Blaser M. J., Wagenaar J. A., Duim B. ( 2013 ). Complete genome sequence of Campylobacter fetus subsp. testudinum strain 03-427T . . Genome Announc 1, e01002-13. [View Article] [PubMed]
    [Google Scholar]
  5. Harvey S., Greenwood J. R. ( 1985 ). Isolation of Campylobacter fetus from a pet turtle. . J Clin Microbiol 21, 260261.[PubMed]
    [Google Scholar]
  6. Hum S., Quinn K., Brunner J., On S. L. ( 1997 ). Evaluation of a PCR assay for identification and differentiation of Campylobacter fetus subspecies. . Aust Vet J 75, 827831. [View Article] [PubMed]
    [Google Scholar]
  7. Linton D., Owen R. J., Stanley J. ( 1996 ). Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals. . Res Microbiol 147, 707718. [View Article] [PubMed]
    [Google Scholar]
  8. Murate T., Banno Y., T-Koizumi K., Watanabe K., Mori N., Wada A., Igarashi Y., Takagi A., Kojima T. & other authors ( 2001 ). Cell type-specific localization of sphingosine kinase 1a in human tissues. . J Histochem Cytochem 49, 845855. [View Article] [PubMed]
    [Google Scholar]
  9. Patrick M. E., Gilbert M. J., Blaser M. J., Tauxe R. V., Wagenaar J. A., Fitzgerald C. ( 2013 ). Human infections with new subspecies of Campylobacter fetus . . Emerg Infect Dis 19, 16781680. [View Article] [PubMed]
    [Google Scholar]
  10. Ribot E. M., Fitzgerald C., Kubota K., Swaminathan B., Barrett T. J. ( 2001 ). Rapid pulsed-field gel electrophoresis protocol for subtyping of Campylobacter jejuni . . J Clin Microbiol 39, 18891894. [View Article] [PubMed]
    [Google Scholar]
  11. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species identification in bacteriology. . International Journal of Systematic Bacteriology 44, 846849. [CrossRef]
    [Google Scholar]
  12. Sebald M., Véron M. ( 1963 ). Teneur en bases de l’ADN et classification des vibrions. . Ann Inst Pasteur (Paris) 105, 897910 (in French).[PubMed]
    [Google Scholar]
  13. Smith T., Taylor M. S. ( 1919 ). Some morphological and biological characters of the spirilla (Vibrio fetus, N. sp.) associated with disease of the fetal membranes in cattle. . J Exp Med 30, 299311. [View Article] [PubMed]
    [Google Scholar]
  14. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Molecular biology and evolution 28, 27312739. [CrossRef]
    [Google Scholar]
  15. Tu Z. C., Dewhirst F. E., Blaser M. J. ( 2001 ). Evidence that the Campylobacter fetus sap locus is an ancient genomic constituent with origins before mammals and reptiles diverged. . Infect Immun 69, 22372244. [View Article] [PubMed]
    [Google Scholar]
  16. Tu Z. C., Hui J., Blaser M. J. ( 2004a ). Conservation and diversity of sap homologues and their organization among Campylobacter fetus isolates. . Infect Immun 72, 17151724. [View Article] [PubMed]
    [Google Scholar]
  17. Tu Z. C., Zeitlin G., Gagner J. P., Keo T., Hanna B. A., Blaser M. J. ( 2004b ). Campylobacter fetus of reptile origin as a human pathogen. . J Clin Microbiol 42, 44054407. [View Article] [PubMed]
    [Google Scholar]
  18. Tu Z. C., Eisner W., Kreiswirth B. N., Blaser M. J. ( 2005 ). Genetic divergence of Campylobacter fetus strains of mammal and reptile origins. . J Clin Microbiol 43, 33343340. [View Article] [PubMed]
    [Google Scholar]
  19. van Bergen M. A., Simons G., van der Graaf-van Bloois L., van Putten J. P., Rombout J., Wesley I., Wagenaar J. A. ( 2005 ). Amplified fragment length polymorphism based identification of genetic markers and novel PCR assay for differentiation of Campylobacter fetus subspecies. . J Med Microbiol 54, 12171224. [View Article] [PubMed]
    [Google Scholar]
  20. Véron M., Chatelain R. ( 1973 ). Taxonomic study of the genus Campylobacter Sebald and Véron and designation of the neotype strain for the type species, Campylobacter fetus (Smith and Taylor) Sebald and Véron. . Int J Syst Bacteriol 23, 122134. [View Article]
    [Google Scholar]
  21. Wang C. M., Shia W. Y., Jhou Y. J., Shyu C. L. ( 2013 ). Occurrence and molecular characterization of reptilian Campylobacter fetus strains isolated in Taiwan. . Vet Microbiol 164, 6776. [View Article] [PubMed]
    [Google Scholar]
  22. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
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