1887

Abstract

A Gram-stain-positive, endospore-forming, rod-shaped, strictly aerobic, moderately halophilic bacterium, designated strain H9B, was isolated from a mud sample of the hypersaline lake Aran-Bidgol in Iran. Cells of strain H9B were motile and produced colonies with a yellowish-grey pigment. Growth occurred between 2.5 and 10 % (w/v) NaCl and the isolate grew optimally at 7.5 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of pH 6–10 and 25–45 °C, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H9B is a member of the genus . The closest relative to this strain was CL-MP28 with 97.1 % 16S rRNA gene sequences similarity. The level of DNA–DNA relatedness between the novel isolate and this phylogenetically related species was 17 %. The major cellular fatty acids of the isolate were anteiso-C, anteiso-C, iso-C and iso-C. The polar lipid pattern of strain H9B consisted of phosphatidylglycerol, diphosphatidylglycerol, four phospholipids and an aminolipid. It contained MK-7 as the predominant menaquinone and -diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 37.1 mol%. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is strain H9B ( = IBRC-M 10780 = KCTC 13823 = CECT 7997).

Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2010-19303)
  • European Funds (FEDER)
  • National Science Foundation (Award DEB-0919290)
  • Junta de Andalucía (Award P10-CVI-6226)
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2014-04-01
2021-10-21
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