1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_2

sp. nov., an algicidal bacterium isolated from red tide seawater Free

Abstract

A Gram-stain-negative, yellow-pigmented, aerobic bacterial strain, designated LY02, was isolated from red tide seawater in Xiamen, Fujian Province, China. Growth was observed at temperatures from 4 to 44 °C, at salinities from 0 to 9 % and at pH from 6 to 10. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus , which belongs to the family . Strain LY02 was related most closely to MSW-14 (97.2 % 16S rRNA gene sequence similarity), followed by JPCCMB0017 (97.1 %), JCS350 (97.1 %) and SW-109 (97.0 %). The dominant fatty acids were Cω7, Cω6 and summed feature 3 (comprising Cω7 and/or Cω6). DNA–DNA hybridization showed that strain LY02 possessed low DNA–DNA relatedness to MSW-14, JPCCMB0017, JCS350 and SW-109 (mean± of 33.2±1.3, 32.1±1.0, 26.7±0.7 and 25.2±1.1 %, respectively). The G+C content of the chromosomal DNA was 61.2 mol%. The predominant respiratory quinone was ubiquinone-10 (Q-10). According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, the novel strain most appropriately belongs to the genus , but can readily be distinguished from recognized species. The name sp. nov. is proposed (type strain LY02 = CGMCC 1.12494 = KCTC 32398 = NBRC 109638).

  • Published Online:
Funding
This study was supported by the:
  • National Nature Science Foundation of China (Award 40930847)
  • Special Fund for PhD Program in University (Award 20120121130001)
  • Public Science and Technology Research Funds Projects of Ocean (Award 201305022 and 201305016)
  • Science and Technology Innovation Funds of Shenzhen (Award JCYJ20120615161239998)
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.057257-0
2014-02-01
2023-09-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/2/631.html?itemId=/content/journal/ijsem/10.1099/ijs.0.057257-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J., Smith J. A., Struhl K. (editors) ( 1995 ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
     [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  3. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  4. DeLong E. F. ( 1992 ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89, 5685–5689. [View Article] [PubMed]
    [Google Scholar]
  5. Fan Z.-Y., Xiao Y.-P., Hui W., Tian G.-R., Lee J.-S., Lee K. C., Quan Z.-X. ( 2011 ). Altererythrobacter dongtanensis sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 61, 2035–2039. [View Article] [PubMed]
    [Google Scholar]
  6. Jeong S. H., Jin H. M., Lee H. J., Jeon C. O. ( 2013 ). Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. . Int J Syst Evol Microbiol 63, 971–976. [View Article] [PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  8. Kumar N. R., Nair S., Langer S., Busse H.-J., Kämpfer P. ( 2008 ). Altererythrobacter indicus sp. nov., isolated from wild rice (Porteresia coarctata Tateoka). . Int J Syst Evol Microbiol 58, 839–844. [View Article] [PubMed]
    [Google Scholar]
  9. Kwon K. K., Woo J.-H., Yang S.-H., Kang J.-H., Kang S. G., Kim S.-J., Sato T., Kato C. ( 2007 ). Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov.. Int J Syst Evol Microbiol 57, 2207–2211. [View Article] [PubMed]
    [Google Scholar]
  10. Lai Q., Yuan J., Shao Z. ( 2009 ). Altererythrobacter marinus sp. nov., isolated from deep seawater. . Int J Syst Evol Microbiol 59, 2973–2976. [View Article] [PubMed]
    [Google Scholar]
  11. Li Y., Wei J., Yang C., Lai Q., Chen Z., Li D., Zhang H., Tian Y., Zheng W., Zheng T. ( 2013 ). Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. . Int J Syst Evol Microbiol 63, 3481–3486. [View Article] [PubMed]
    [Google Scholar]
  12. Liu C., Shao Z. ( 2005 ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol 55, 1181–1186. [View Article] [PubMed]
    [Google Scholar]
  13. Matsumoto M., Iwama D., Arakaki A., Tanaka A., Tanaka T., Miyashita H., Matsunaga T. ( 2011 ). Altererythrobacter ishigakiensis sp. nov., an astaxanthin-producing bacterium isolated from a marine sediment. . Int J Syst Evol Microbiol 61, 2956–2961. [View Article] [PubMed]
    [Google Scholar]
  14. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297–306. [View Article] [PubMed]
    [Google Scholar]
  15. Nedashkovskaya O. I., Cho S.-H., Joung Y., Joh K., Kim M. N., Shin K.-S., Oh H. W., Bae K. S., Mikhailov V. V., Kim S. B. ( 2013 ). Altererythrobacter troitsensis sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . . Int J Syst Evol Microbiol 63, 93–97. [View Article] [PubMed]
    [Google Scholar]
  16. Park S. C., Baik K. S., Choe H. N., Lim C. H., Kim H. J., Ka J.-O., Seong C. N. ( 2011 ). Altererythrobacter namhicola sp. nov. and Altererythrobacter aestuarii sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61, 709–715. [View Article] [PubMed]
    [Google Scholar]
  17. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  18. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  19. Seo S. H., Lee S. D. ( 2010 ). Altererythrobacter marensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 60, 307–311. [View Article] [PubMed]
    [Google Scholar]
  20. Su J., Zhou Y., Lai Q., Li X., Dong P., Yang X., Zhang B., Zhang J., Zheng X. & other authors ( 2013 ). Sinobacterium caligoides gen. nov., sp. nov., a new member of the family Oceanospirillaceae isolated from the South China Sea, and emended description of Amphritea japonica . . Int J Syst Evol Microbiol 63, 2095–2100. [View Article] [PubMed]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  23. Xue X., Zhang K., Cai F., Dai J., Wang Y., Rahman E., Peng F., Fang C. ( 2012 ). Altererythrobacter xinjiangensis sp. nov., isolated from desert sand, and emended description of the genus Altererythrobacter . . Int J Syst Evol Microbiol 62, 28–32. [View Article] [PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kang K. H., Yeo S.-H., Oh T.-K. ( 2005 ). Erythrobacter luteolus sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 55, 1167–1170. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.057257-0
Loading
Altererythrobacter xiamenensis sp. nov., an algicidal bacterium isolated from red tide seawater
Int J Syst Evol Microbiol 64, 631 (2014); https://doi.org/10.1099/ijs.0.057257-0
/content/journal/ijsem/10.1099/ijs.0.057257-0
/content/journal/ijsem/10.1099/ijs.0.057257-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error