1887

Abstract

The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2 and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with and the closest relatedness to FWC 38, ATCC 15257 and H-E3-2 showing 98.5 %, 97.3 % and 97.3 % 16S rRNA gene sequence similarity, respectively, and 96.1–96.7 % similarity to all other species of the genus . The two isolates shared 100 % 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (Cω6 and/or Cω7), C, Cω7 11-methyl and summed feature 3 (Cω6 and/or Cω7). The G+C contents of the genomic DNA of strains DS48-5-2 and DS48-6-3 were 66.7 mol% and 66.2 mol%, respectively. DNA–DNA hybridization values of strains DS48-5-2 and DS48-6-3 with FWC 38, ATCC 15257 and H-E3-2 were 19.3 %–24.4 %. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2 and DS48-6-3 are representatives of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is DS48-5-2 ( = KCTC 32480 = JCM 19440).

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2014-03-01
2020-01-27
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References

  1. Abraham W.-R., Strömpl C., Meyer H., Lindholst S., Moore E. R. B., Christ R., Vancanneyt M., Tindall B. J., Bennasar A.. & other authors ( 1999;). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. . Int J Syst Bacteriol 49:, 1053–1073. [CrossRef][PubMed]
    [Google Scholar]
  2. Abraham W.-R., Macedo A. J., Lünsdorf H., Fischer R., Pawelczyk S., Smit J., Vancanneyt M.. ( 2008;). Phylogeny by a polyphasic approach of the order Caulobacterales, proposal of Caulobacter mirabilis sp. nov., Phenylobacterium haematophilum sp. nov. and Phenylobacterium conjunctum sp. nov., and emendation of the genus Phenylobacterium. . Int J Syst Evol Microbiol 58:, 1939–1949. [CrossRef][PubMed]
    [Google Scholar]
  3. Bates R. G., Bower V. E.. ( 1956;). Alkaline solutions for pH control. . Anal Chem 28:, 1322–1324. [CrossRef]
    [Google Scholar]
  4. Chen H., Jogler M., Rohde M., Klenk H. P., Busse H.-J., Tindall B.-J., Spröer C., Overmann J.. ( 2012;). Reclassification and emended description of Caulobacter leidyi as Sphingomonas leidyi comb. nov., and emendation of the genus Sphingomonas. . Int J Syst Evol Microbiol 62:, 2835–2843. [CrossRef][PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid- deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Gomori G.. ( 1955;). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1:, 138–146. [CrossRef]
    [Google Scholar]
  10. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucl Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  11. Henrici A. T., Johnson D. E.. ( 1935;). Studies of fresh water bacteria. II. Stalked bacteria, a new order of schizomycetes. . J Bacteriol 30:, 61–93.[PubMed]
    [Google Scholar]
  12. Jin L., Lee H. G., No K. J., Ko S.-R., Kim H. S., Ahn C. Y., Oh H. M.. ( 2013a;). Belnapia soli sp. nov., a proteobacterium isolated from grass soil. . Int J Syst Evol Microbiol 63:, 1955–1959. [CrossRef][PubMed]
    [Google Scholar]
  13. Jin L., Lee H.-G., Kim H.-S., Ahn C.-Y., Oh H.-M.. ( 2013b;). Caulobacter daechungensis sp. nov., a stalked bacterium isolated from a eutrophic reservoir. . Int J Syst Evol Microbiol 63:, 2559–2564. [CrossRef][PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  15. Komagata K., Suzuki K.-I.. ( 1987;). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  16. Liu Q.-M., Ten L. N., Im W.-T., Lee S.-T., Yoon M.-H.. ( 2010;). Caulobacter ginsengisoli sp. nov., a novel stalked bacterium isolated from ginseng cultivating soil. . J Microbiol Biotechnol 20:, 15–20.[PubMed]
    [Google Scholar]
  17. Löeffler F.. ( 1890;). Weitere Untersuchungen uber die Beizung und Farbung der Geisseln bei den Bakterien. . Centralbl Bakteriol Parasitenkd 7:, 625–639.
    [Google Scholar]
  18. Poindexter J. S.. ( 1964;). Biological properties and classification of the Caulobacter group. . Bacteriol Rev 28:, 231–295.[PubMed]
    [Google Scholar]
  19. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  21. Staley J. T.. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  22. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reverse-phased high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  25. Urakami T., Oyanagi H., Araki H., Suzuki K. I., Komagata K.. ( 1990;). Recharacterization and emended description of the genus Mycoplana and description of two new species, Mycoplana ramosa and Mycoplana segnis. . Int J Syst Bacteriol 40:, 434–442. [CrossRef]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
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