1887

Abstract

A novel, Gram-stain-positive bacterium, designated strain EX-07, was isolated from (Korean traditional food). The strain was aerobic, halotolerant and non-motile; it formed cocci that grouped into tetrads and sarcinae or formed irregular conglomerates. Growth occurred at pH 7–9, at 10–37 °C and with up to 9 % NaCl. Isolate EX-07 was catalase- and oxidase-negative and used sugars and organic acids as carbon sources. The 16S rRNA gene sequence of the novel strain showed 94.2–94.5 % similarity with the type strains of , , and and only 92.2 % with representatives of the genera , and . Sequence similarities based on the gene ranged from 81.3 to 82.8 % between the novel isolate and the type strains of all species of the genus , and only 74.2 and 75.3 % with type strains of members of the genera and , respectively. The G+C content of the genomic DNA was 39.6 mol%. The predominant fatty acids were Cω9, Cω9, C and C. The polar lipid profile was very complex and included phosphatidylethanolamine and several unidentified aminolipids, glycolipids and phospholipids. Based on the genotypic and phenotypic results obtained in this study, it is proposed that isolate EX-07 represents a novel species of a new genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of is EX-07 ( = KCCM 90229 = JCM 19215).

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2014-05-01
2019-12-06
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