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Volume 64, Issue Pt_5

Description of a Gram-negative bacterium, sp. nov. Free

Abstract

A Gram-stain-negative bacterial strain, designated 9NM-8, was isolated from an abandoned lead-zinc ore in Mei county, Meizhou, Guangdong province, PR China. The isolate was orange-pigmented, aerobic, oxidase- and catalase-positive, motile with lophotrichous flagella and rod-shaped. Strain 9NM-8 grew optimally at pH 7.0 and 30 °C and in the absence of NaCl on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 9NM-8 belongs to the genus , with highest sequence similarities to KACC 14484 (96.1 %), DSM 7225 (96.0 %) and DSM 13101 (95.6 %). Strain 9NM-8 contained Q-10 as the predominant ubiquinone. The major fatty acids included Cω7, C, Cω7 and/or Cω6 (summed feature 3) and 11-methyl Cω7. The DNA G+C content was 69.6±1.3 mol%. The major component in the polyamine pattern was -homospermidine and the polar lipid profile contained sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and two unidentified phospholipids. Based on comparative analysis of physiological, chemotaxonomic and phylogenetic characteristics, strain 9NM-8 should be considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 9NM-8 ( = GIMCC 1.653 = CGMCC 1.12672 = DSM 27570).

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Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 31200006 and 31070103)
  • Key Project of Guangdong Natural Science Foundation (Award 10251007002000001)
© 2014 IUMS
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Description of a Gram-negative bacterium, Sphingomonas guangdongensis sp. nov.
Int J Syst Evol Microbiol 64, 1697 (2014); https://doi.org/10.1099/ijs.0.056853-0
/content/journal/ijsem/10.1099/ijs.0.056853-0
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