1887

Abstract

A novel endophytic actinomycete, designated strain NEAU-LZS-5, was isolated from the leaf of candelabra aloe ( Mill) and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain NEAU-LZS-5 belongs to the genus and exhibited 99.51 and 97.37 % similarity to YIM 65188 and GW41-1564, respectively, whereas low similarity values (<97 %) distinguished strain NEAU-LZS-5 from all other species of the genus with validly published names. Two tree-making algorithms also supported the position that strain NEAU-LZS-5 formed a distinct clade with YIM 65188 and GW41-1564. However, levels of DNA–DNA relatedness between strain NEAU-LZS-5 and YIM 65188 and GW41-1564 were 45.59 and 31.90 %, respectively. A comparative study between strain NEAU-LZS-5 and the type strains of closest related species of the genus revealed that it differed from them in morphological, physiological and biochemical characteristics. Therefore, strain NEAU-LZS-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-LZS-5 ( = CGMCC 4.7095 = DSM 42101).

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2014-04-01
2019-10-14
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References

  1. Anderson A. S., Wellington E. M. H.. ( 2001;). The taxonomy of Streptomyces and related genera. . Int J Syst Evol Microbiol 51:, 797–814. [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D.. ( 1985;). Isoprenoid quinone analyses in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–284. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–789. [CrossRef]
    [Google Scholar]
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  7. Hayakawa M., Nonomura H.. ( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  8. Huss V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  9. Kämpfer P., Huber B., Buczolits S., Thummes K., Grün-Wollny I., Busse H.-J.. ( 2008;). Streptomyces specialis sp. nov.. Int J Syst Evol Microbiol 58:, 2602–2606. [CrossRef][PubMed]
    [Google Scholar]
  10. Kelly K. L.. ( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  11. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M.. ( 2000;). Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. . Int J Syst Evol Microbiol 50:, 2031–2036. [CrossRef][PubMed]
    [Google Scholar]
  12. Kim S. B., Lonsdale J., Seong C. N., Goodfellow M.. ( 2003;). Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman and Henrici (1943)AL) emend. Rainey et al. 1997. . Antonie van Leeuwenhoek 83:, 107–116. [CrossRef][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  14. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  15. Lechevalier H. A., Lechevalier M. P.. ( 1970a;). A critical evaluation of the genera of aerobic actinomycetes. . In The Actinomycetes, pp. 393–405. Edited by Prauser H... Jena:: Gustav Fischer Verlag;.
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A.. ( 1970b;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A.. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no. 6), pp. 277–284. Edited by Dietz A., Thayer J... Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  18. Lechevalier M. P., De Bièvre C., Lechevalier H. A.. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  19. Li J., Zhao G. Z., Qin S., Zhu W. Y., Xu L. H., Li W. J.. ( 2009;). Streptomyces sedi sp. nov., isolated from surface-sterilized roots of Sedum sp.. Int J Syst Evol Microbiol 59:, 1492–1496. [CrossRef][PubMed]
    [Google Scholar]
  20. Locci R.. ( 1989;). Streptomyces and related genera. . In Bergey’s Manual of Systematic Bacteriolgy, vol. 4, pp. 2451–2508. Edited by Williams S. T., Sharpe M. E., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  21. Mandel M., Marmur J.. ( 1968;). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B:, 195–206. [CrossRef]
    [Google Scholar]
  22. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J.. ( 2000;). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30:, 178–182. [CrossRef][PubMed]
    [Google Scholar]
  23. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M.. ( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188:, 221–233. [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  25. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. . Int Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  27. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  29. Waksman S. A.. ( 1961;). The Actinomycetes, vol. 2. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  30. Waksman S. A.. ( 1967;). The Actinomycetes. A Summary of Current Knowledge. New York:: Ronald;.
    [Google Scholar]
  31. Waksman S. A., Henrici A. T.. ( 1943;). The nomenclature and classification of the actinomycetes. . J Bacteriol 46:, 337–341.[PubMed]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  33. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  34. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y.. ( 2011;). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). . Int J Syst Evol Microbiol 61:, 1165–1169. [CrossRef][PubMed]
    [Google Scholar]
  35. Yokota A., Tamura T., Hasegawa T., Huang L. H.. ( 1993;). Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. . Int J Syst Bacteriol 43:, 805–812. [CrossRef]
    [Google Scholar]
  36. Zhang Z., Wang Y., Ruan J.. ( 1997;). A proposal to revive the genus Kitasatospora (Omura, Takahashi, Iwai, and Tanaka 1982). . Int J Syst Bacteriol 47:, 1048–1054. [CrossRef][PubMed]
    [Google Scholar]
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