Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_4

Description of gen. nov., sp. nov., isolated from flounder (), reclassification of four species of the genus as members of the genus gen. nov. and emended description of the genus Free

Abstract

A Gram-staining-negative, aerobic, rod-shaped bacterium, designated strain T202, was isolated from the gill of a cultured flounder (). Based on 16S rRNA gene sequence similarity, strain T202 was a member of the family and shared 93.32–96.58 % similarity with type strains of all members of the most closely related genus . Phylogenetically, the isolate shared a root with the type strains of four marine species, M-M1, TMA1, CBMAI 722 and JC2041. Optimal growth occurred in the presence of 2–4 % (w/v) NaCl, at pH 7.0–8.0 and at 28 °C. Ubiquinone 8 (Q-8) was the predominant respiratory quinone. The major fatty acids were C, summed feature 3 (Cω7 and/or Cω6), Cω9 and Cω8 The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain T202 was 37 mol%. On the basis of polyphasic analysis, especially the phylogenetic relationships and the lower DNA G+C content, strain T202 is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is T202 ( = JCM 18590 = DSM 26287 = KCTC 32144). Because M-M1, TMA1, CBMAI 722 and JC2041 formed a phylogenetic group together with strain T202 that was clearly separated from other known strains of , these four species are reclassified as members of the genus as comb. nov. (type strain M-M1 = KCTC 23343 = CCUG 60020), comb. nov. (type strain TMA1 = BCRC 17492 = JCM 13379 = DSM 19706), comb. nov. (type strain CBMAI 722 = LMG 22536) and comb. nov. (type strain JC2041 = IMSNU 14005 = KCTC 12041 = DSM 15355). The type species of the genus is gen. nov., comb. nov. An emended description of the genus is also proposed.

  • Published Online:
Funding
This study was supported by the:
  • International Science and Technology Cooperation Program of China (Award 2012DFG31990)
  • National High Technology Research and Development Program of China (863 program) (Award 2007AA09Z434)
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055913-0
2014-04-01
2025-01-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1223.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055913-0&mimeType=html&fmt=ahah

References

  1. Beveridge T., Lawrence J., Murray R. ( 2007 ). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 19–33. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  2. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  3. Hosoya S., Adachi K., Kasai H. ( 2009 ). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59, 686–690. [View Article] [PubMed]
    [Google Scholar]
  4. Jean W. D., Shieh W. Y., Liu T. Y. ( 2006 ). Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas . . Int J Syst Evol Microbiol 56, 1245–1250. [View Article] [PubMed]
    [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  6. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207.
     [Google Scholar]
  8. Lyman J., Fleming R. H. ( 1940 ). Composition of seawater. . J Mar Res 3, 134–146.
     [Google Scholar]
  9. Macián M. C., Ludwig W., Schleifer K. H., Garay E., Pujalte M. J. ( 2001 ). Thalassomonas viridans gen. nov., sp. nov., a novel marine γ-proteobacterium. . Int J Syst Evol Microbiol 51, 1283–1289. [View Article] [PubMed]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  11. Minnikin D., O’Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  12. Montero-Calasanz M. del C., Göker M., Rohde M., Spröer C., Schumann P., Busse H.-J., Schmid M., Tindall B. J., Klenk H.-P., Camacho M. ( 2013 ). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . . Int J Syst Evol Microbiol 63, 4386–4395. [CrossRef]
    [Google Scholar]
  13. Moore E. R. B., Arnscheidt A., Krüger A., Strömpl C., Mau M. ( 1999 ). Simplified protocols for the preparation of genomic DNA from bacterial cultures. . In Molecular Microbial Ecology Manual, pp. 1.6.1.1–1.6.1.15. Edited by Akkermans A. D. L., van Elsas J. D., de Bruijn F. J. . Dordrecht:: Kluwer;.
     [Google Scholar]
  14. Park S., Choi W. C., Oh T. K., Yoon J. H. ( 2011 ). Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. . Int J Syst Evol Microbiol 61, 2573–2576. [View Article] [PubMed]
    [Google Scholar]
  15. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
     [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  17. Teather R. M., Wood P. J. ( 1982 ). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol 43, 777–780.[PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  19. Thompson F. L., Barash Y., Sawabe T., Sharon G., Swings J., Rosenberg E. ( 2006 ). Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. . Int J Syst Evol Microbiol 56, 365–368. [View Article] [PubMed]
    [Google Scholar]
  20. Tindall B., Sikorski J., Smibert R., Krieg N. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  21. Xie C.-H., Yokota A. ( 2003 ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49, 345–349. [View Article] [PubMed]
    [Google Scholar]
  22. Yi H., Bae K. S., Chun J. ( 2004 ). Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 54, 377–380. [View Article] [PubMed]
    [Google Scholar]
  23. Yu T., Yin Q., Song X., Zhao R., Shi X., Zhang X.-H. ( 2013 ). Aquimarina longa sp. nov., isolated from seawater, and emended description of Aquimarina muelleri . . Int J Syst Evol Microbiol 63, 1235–1240. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.055913-0
Loading
Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas
Int J Syst Evol Microbiol 64, 1223 (2014); https://doi.org/10.1099/ijs.0.055913-0
/content/journal/ijsem/10.1099/ijs.0.055913-0
/content/journal/ijsem/10.1099/ijs.0.055913-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error