1887

Abstract

A Gram-staining-negative, aerobic, rod-shaped bacterium, designated strain T202, was isolated from the gill of a cultured flounder (). Based on 16S rRNA gene sequence similarity, strain T202 was a member of the family and shared 93.32–96.58 % similarity with type strains of all members of the most closely related genus . Phylogenetically, the isolate shared a root with the type strains of four marine species, M-M1, TMA1, CBMAI 722 and JC2041. Optimal growth occurred in the presence of 2–4 % (w/v) NaCl, at pH 7.0–8.0 and at 28 °C. Ubiquinone 8 (Q-8) was the predominant respiratory quinone. The major fatty acids were C, summed feature 3 (Cω7 and/or Cω6), Cω9 and Cω8 The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain T202 was 37 mol%. On the basis of polyphasic analysis, especially the phylogenetic relationships and the lower DNA G+C content, strain T202 is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is T202 ( = JCM 18590 = DSM 26287 = KCTC 32144). Because M-M1, TMA1, CBMAI 722 and JC2041 formed a phylogenetic group together with strain T202 that was clearly separated from other known strains of , these four species are reclassified as members of the genus as comb. nov. (type strain M-M1 = KCTC 23343 = CCUG 60020), comb. nov. (type strain TMA1 = BCRC 17492 = JCM 13379 = DSM 19706), comb. nov. (type strain CBMAI 722 = LMG 22536) and comb. nov. (type strain JC2041 = IMSNU 14005 = KCTC 12041 = DSM 15355). The type species of the genus is gen. nov., comb. nov. An emended description of the genus is also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055913-0
2014-04-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1223.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055913-0&mimeType=html&fmt=ahah

References

  1. Beveridge T. , Lawrence J. , Murray R. . ( 2007; ). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 19–33. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M. , Snyder L. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  2. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  3. Hosoya S. , Adachi K. , Kasai H. . ( 2009; ). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59:, 686–690. [CrossRef] [PubMed]
    [Google Scholar]
  4. Jean W. D. , Shieh W. Y. , Liu T. Y. . ( 2006; ). Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas . . Int J Syst Evol Microbiol 56:, 1245–1250. [CrossRef] [PubMed]
    [Google Scholar]
  5. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  6. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  7. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207.
    [Google Scholar]
  8. Lyman J. , Fleming R. H. . ( 1940; ). Composition of seawater. . J Mar Res 3:, 134–146.
    [Google Scholar]
  9. Macián M. C. , Ludwig W. , Schleifer K. H. , Garay E. , Pujalte M. J. . ( 2001; ). Thalassomonas viridans gen. nov., sp. nov., a novel marine γ-proteobacterium. . Int J Syst Evol Microbiol 51:, 1283–1289. [CrossRef] [PubMed]
    [Google Scholar]
  10. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  11. Minnikin D. , O’Donnell A. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  12. Montero-Calasanz M. del C. , Göker M. , Rohde M. , Spröer C. , Schumann P. , Busse H.-J. , Schmid M. , Tindall B. J. , Klenk H.-P. , Camacho M. . ( 2013; ). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . . Int J Syst Evol Microbiol 63:, 4386–4395.[CrossRef]
    [Google Scholar]
  13. Moore E. R. B. , Arnscheidt A. , Krüger A. , Strömpl C. , Mau M. . ( 1999; ). Simplified protocols for the preparation of genomic DNA from bacterial cultures. . In Molecular Microbial Ecology Manual, pp. 1.6.1.1–1.6.1.15. Edited by Akkermans A. D. L. , van Elsas J. D. , de Bruijn F. J. . . Dordrecht:: Kluwer;.
    [Google Scholar]
  14. Park S. , Choi W. C. , Oh T. K. , Yoon J. H. . ( 2011; ). Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. . Int J Syst Evol Microbiol 61:, 2573–2576. [CrossRef] [PubMed]
    [Google Scholar]
  15. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  16. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  17. Teather R. M. , Wood P. J. . ( 1982; ). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol 43:, 777–780.[PubMed]
    [Google Scholar]
  18. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  19. Thompson F. L. , Barash Y. , Sawabe T. , Sharon G. , Swings J. , Rosenberg E. . ( 2006; ). Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. . Int J Syst Evol Microbiol 56:, 365–368. [CrossRef] [PubMed]
    [Google Scholar]
  20. Tindall B. , Sikorski J. , Smibert R. , Krieg N. . ( 2007; ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M. , Snyder L. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Xie C.-H. , Yokota A. . ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef] [PubMed]
    [Google Scholar]
  22. Yi H. , Bae K. S. , Chun J. . ( 2004; ). Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 54:, 377–380. [CrossRef] [PubMed]
    [Google Scholar]
  23. Yu T. , Yin Q. , Song X. , Zhao R. , Shi X. , Zhang X.-H. . ( 2013; ). Aquimarina longa sp. nov., isolated from seawater, and emended description of Aquimarina muelleri . . Int J Syst Evol Microbiol 63:, 1235–1240. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.055913-0
Loading
/content/journal/ijsem/10.1099/ijs.0.055913-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error