1887

Abstract

A novel exopolysaccharide-producing bacterium, designated strain SE3, was isolated from Pacific Ocean sediment. The strain was Gram-stain-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of Cω7, C and Cω7. The DNA G+C content was 46.9 mol%. 16S rRNA gene sequence analysis suggested that strain SE3 is a member of the genus . Strain SE3 exhibited close phylogenetic affinity to JCM 17292 (99.0 % 16S rRNA gene sequence similarity), LMEB 39 (98.39 %) and HJ51 (97.65 %). The DNA–DNA reassociation values between strain SE3 and JCM 17292, JCM 15903 and LMG 24469 were 31, 26 and 44 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA relatedness data, the new isolate merits classification as a representative of novel species, for which the name is proposed. The type strain is SE3 ( = JCM 18891 = NCIMB 14852).

Funding
This study was supported by the:
  • , Ministry of Education, Science, Sports and Culture, Japan , (Award 2241001)
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2014-01-01
2020-08-13
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