1887

Abstract

A novel exopolysaccharide-producing bacterium, designated strain SE3, was isolated from Pacific Ocean sediment. The strain was Gram-stain-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of Cω7, C and Cω7. The DNA G+C content was 46.9 mol%. 16S rRNA gene sequence analysis suggested that strain SE3 is a member of the genus . Strain SE3 exhibited close phylogenetic affinity to JCM 17292 (99.0 % 16S rRNA gene sequence similarity), LMEB 39 (98.39 %) and HJ51 (97.65 %). The DNA–DNA reassociation values between strain SE3 and JCM 17292, JCM 15903 and LMG 24469 were 31, 26 and 44 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA relatedness data, the new isolate merits classification as a representative of novel species, for which the name is proposed. The type strain is SE3 ( = JCM 18891 = NCIMB 14852).

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2014-01-01
2019-10-20
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References

  1. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Baumann L. , Baumann P. , Mandel M. , Allen R. D. . ( 1972; ). Taxonomy of aerobic marine eubacteria. . J Bacteriol 110:, 402–429.[PubMed]
    [Google Scholar]
  3. Bowman J. P. , McMeekin T. A. . ( 2005; ). Genus Xl. Pseudoalteromonas Gauthier, Gauthier and Christen 1995a, 759VP . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol 2, pp. 467–478. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  4. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used determine genetic relatedness among bacterial strains. . Int J Syst Evol Microbiol 39:, 224–229.
    [Google Scholar]
  5. Gauthier G. , Gauthier M. , Christen R. . ( 1995; ). Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. . Int J Syst Bacteriol 45:, 755–761. [CrossRef] [PubMed]
    [Google Scholar]
  6. Guindon S. , Gascuel O. . ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef] [PubMed]
    [Google Scholar]
  7. Ivanova E. P. , Zhukova N. V. , Svetashev V. I. , Gorshkova N. M. , Kurilenko V. V. , Frolova G. M. , Mikhailov V. V. . ( 2000; ). Evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of Alteromonas-like proteobacteria. . Curr Microbiol 41:, 341–345. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  10. Leifson E. . ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  11. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  12. Matsuyama H. , Kamesaki T. , Sasaki R. , Minami H. , Yumoto I. . ( 2003; ). Production of two types of exopolysaccharide by Novosphingobium rosa . . J Biosci Bioeng 95:, 152–156.[PubMed] [CrossRef]
    [Google Scholar]
  13. Matsuyama H. , Hirabayashi T. , Kasahara H. , Minami H. , Hoshino T. , Yumoto I. . ( 2006; ). Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. . Int J Syst Evol Microbiol 56:, 2883–2886. [CrossRef] [PubMed]
    [Google Scholar]
  14. Matsuyama H. , Minami H. , Kasahara H. , Kato Y. , Murayama M. , Yumoto I. . ( 2013; ). Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium. . Int J Syst Evol Microbiol 63:, 1805–1809. [CrossRef] [PubMed]
    [Google Scholar]
  15. Nishijima M. , Araki-Sakai M. , Sano H. . ( 1997; ). Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. . J Microbiol Methods 28:, 113–122. [CrossRef]
    [Google Scholar]
  16. Oh Y.-S. , Park A.-R. , Lee J.-K. , Lim C.-S. , Yoo J.-S. , Roh D.-H. . ( 2011; ). Pseudoalteromonas donghaensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61:, 351–355. [CrossRef] [PubMed]
    [Google Scholar]
  17. Rzhetsky A. , Nei M. . ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10:, 1073–1095.[PubMed]
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  20. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  21. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  22. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. e. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  23. Xu X.-W. , Wu Y.-H. , Wang C.-S. , Gao X.-H. , Wang X.-G. , Wu M. . ( 2010; ). Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary. . Int J Syst Evol Microbiol 60:, 2176–2181. [CrossRef] [PubMed]
    [Google Scholar]
  24. Yumoto I. , Yamazaki K. , Hishinuma M. , Nodasaka Y. , Suemori A. , Nakajima K. , Inoue N. , Kawasaki K. . ( 2001; ). Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. . Int J Syst Evol Microbiol 51:, 349–355.[PubMed]
    [Google Scholar]
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