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Abstract

A novel strain designated MRP-15, belonging to the class (phylum ), was isolated from the rhizoplane of in South Korea and was characterized taxonomically using a polyphasic approach. The strain was found to comprise Gram-stain-negative, aerobic, non-motile, non-spore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus but was clearly separated from established species of the genus . 16S rRNA gene sequence similarities between strain MRP-15 and type strains of established species of the genus ranged from 90.3 to 97.8 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C and Cω5) supported the affiliation of strain MRP-15 with the genus . Therefore strain MRP-15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MRP-15 ( = KACC 17184 = NCAIMB 02530).

Funding
This study was supported by the:
  • Regional SubGenBank Support Program of Rural Development Administration, Republic of Korea
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2014-01-01
2024-05-06
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References

  1. Bligh E. G., Dyer W. J. ( 1959 ). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37, 911917. [View Article] [PubMed]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  6. Hiraishi A., Ueda Y., Ishihara J., Mori T. ( 1996 ). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. . J Gen Appl Microbiol 42, 457469. [View Article]
    [Google Scholar]
  7. Kämpfer P., Young C. C., Sridhar K. R., Arun A. B., Lai W. A., Shen F. T., Rekha P. D. ( 2006 ). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov.. Int J Syst Evol Microbiol 56, 22232228. [View Article] [PubMed]
    [Google Scholar]
  8. Kim M. K., Jung H.-Y. ( 2007 ). Chitinophaga terrae sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57, 17211724. [View Article] [PubMed]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  10. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  11. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  12. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. ( 2007 ). Clustal W and Clustal X version 2.0. . Bioinformatics 23, 29472948. [View Article] [PubMed]
    [Google Scholar]
  13. Lee H.-G., An D.-S., Im W.-T., Liu Q.-M., Na J.-R., Cho D. H., Jin C. W., Lee S.-T., Yang D.-C. ( 2007 ). Chitinophaga ginsengisegetis sp. nov. and Chitinophaga ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 57, 13961401. [View Article] [PubMed]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  15. Moore D. D., Dowhan D. ( 1995 ). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 211. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Wiley;.
    [Google Scholar]
  16. Murray R. G. E., Doetsch R. N., Robinow F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Pankratov T. A., Kulichevskaya I. S., Liesack W., Dedysh S. N. ( 2006 ). Isolation of aerobic, gliding, xylanolytic and laminarinolytic bacteria from acidic Sphagnum peatlands and emended description of Chitinophaga arvensicola Kämpfer et al. 2006. . Int J Syst Evol Microbiol 56, 27612764. [View Article] [PubMed]
    [Google Scholar]
  18. Reichenbach H. ( 1989 ). Genus I. Cytophaga Winogradsky 1929, 577, AL emend. . In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 20152050. Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. C. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  19. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  20. Sangkhobol V., Skerman V. B. D. ( 1981 ). Chitinophaga, a new genus of chitinolytic myxobacteria. . Int J Syst Bacteriol 31, 285293. [View Article]
    [Google Scholar]
  21. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids . , MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  23. Weon H.-Y., Yoo S.-H., Kim Y.-J., Son J.-A., Kim B.-Y., Kwon S.-W., Koo B. S. ( 2009 ). Chitinophaga niabensis sp. nov. and Chitinophaga niastensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59, 12671271. [View Article] [PubMed]
    [Google Scholar]
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