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Volume 63, Issue Pt_12

sp. nov., isolated from an electroactive biofilm, and emended description of the genus Migula 1894 Free

Abstract

A Gram-negative, straight to slightly curved rod-shaped bacterium, motile with peritrichous flagella, designated SgZ-6, was isolated from an electroactive biofilm and was characterized by means of a polyphasic approach. Growth occurred with 0–5.0 % (w/v) NaCl (optimum 1 %), at pH 6.0–10.0 (optimum pH 7.0) and at 10–42 °C (optimum 30 °C) in trypticase soya broth. Phylogenetic analyses based on the 16S rRNA and genes identified the isolate as a member of a novel species of the genus . Strain SgZ-6 exhibited the highest 16S rRNA gene sequence similarity to ‘ ’ CGMCC 1.10701 (97.5 %), followed by JCM 18195 (97.4 %), DSM 21016 (96.6 %), JCM 14085 (96.5 %) and JCM 10630 (96.4 %). Strain SgZ-6 showed the highest gene sequence similarity of 93.7 % to ‘’ CGMCC 1.10701 among all type strains of genus . DNA–DNA pairing studies showed that strain SgZ-6 displayed 47.1 and 40.3 % relatedness to ‘’ CGMCC 1.10701 and JCM 18195, respectively. The major isoprenoid quinone was ubiquinone 9 (Q-9). The whole-cell fatty acids consisted mainly of summed feature 3 (Cω6 and/or Cω7), C and summed feature 8 (Cω6 and/or Cω7). The DNA G+C content of the genomic DNA was 68.1 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain SgZ-6 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SgZ-6 ( = CCTCC AB 2012022 = KACC 16606). An emended description of the genus is also proposed.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41171205 and 41222006)
  • Team Project of Guangdong Natural Science Foundation (Award S2012030006114 and S2011030002882)
© 2013 IUMS
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2013-12-01
2024-04-26
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Pseudomonasguangdongensis sp. nov., isolated from an electroactive biofilm, and emended description of the genus Pseudomonas Migula 1894
Int J Syst Evol Microbiol 63, 4599 (2013); https://doi.org/10.1099/ijs.0.054676-0
/content/journal/ijsem/10.1099/ijs.0.054676-0
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