1887

Abstract

A Gram-negative, straight to slightly curved rod-shaped bacterium, motile with peritrichous flagella, designated SgZ-6, was isolated from an electroactive biofilm and was characterized by means of a polyphasic approach. Growth occurred with 0–5.0 % (w/v) NaCl (optimum 1 %), at pH 6.0–10.0 (optimum pH 7.0) and at 10–42 °C (optimum 30 °C) in trypticase soya broth. Phylogenetic analyses based on the 16S rRNA and genes identified the isolate as a member of a novel species of the genus . Strain SgZ-6 exhibited the highest 16S rRNA gene sequence similarity to ‘ ’ CGMCC 1.10701 (97.5 %), followed by JCM 18195 (97.4 %), subsp. DSM 21016 (96.6 %), JCM 14085 (96.5 %) and JCM 10630 (96.4 %). Strain SgZ-6 showed the highest gene sequence similarity of 93.7 % to ‘’ CGMCC 1.10701 among all type strains of genus . DNA–DNA pairing studies showed that strain SgZ-6 displayed 47.1 and 40.3 % relatedness to ‘’ CGMCC 1.10701 and JCM 18195, respectively. The major isoprenoid quinone was ubiquinone 9 (Q-9). The whole-cell fatty acids consisted mainly of summed feature 3 (Cω6 and/or Cω7), C and summed feature 8 (Cω6 and/or Cω7). The DNA G+C content of the genomic DNA was 68.1 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain SgZ-6 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SgZ-6 ( = CCTCC AB 2012022 = KACC 16606). An emended description of the genus is also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.054676-0
2013-12-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4599.html?itemId=/content/journal/ijsem/10.1099/ijs.0.054676-0&mimeType=html&fmt=ahah

References

  1. Baker G. C., Smith J. J., Cowan D. A.. ( 2003;). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55:, 541–555. [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  3. Dong X., Cai M.. ( 2001;). Manual of Systematic and Determinative Bacteriology. Beijing:: Academic Press;.
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. He W. H., Wang Y. N., Du X., Zhou Y., Jia B., Bian J., Liu S. J., Chen G. C.. ( 2012;). Pseudomonas linyingensis sp. nov.: a novel bacterium isolated from wheat soil subjected to long-term herbicides application. . Curr Microbiol 65:, 595–600. [CrossRef][PubMed]
    [Google Scholar]
  6. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  7. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  8. Lin S. Y., Hameed A., Liu Y. C., Hsu Y. H., Lai W. A., Chen W. M., Shen F. T., Young C. C.. ( 2013;). Pseudomonas sagittaria sp. nov., a siderophore-producing bacterium isolated from oil-contaminated soil. . Int J Syst Evol Microbiol 63:, 2410–2417. [CrossRef][PubMed]
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  10. Migula W.. ( 1894;). Über ein neues System der Bakterien. . Arb Bakteriol Inst Karlsruhe 1:, 235–238 (in German).
    [Google Scholar]
  11. Palleroni N. J.. ( 2005;). Genus I. Pseudomonas Migula 1894, 237AL (nom. cons., Opin. 5 of the Jud. Comm. 1952, 121). . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2B, pp. 323–379. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M... New York:: Springer;.
    [Google Scholar]
  12. Pandey K. K., Mayilraj S., Chakrabarti T.. ( 2002;). Pseudomonas indica sp. nov., a novel butane-utilizing species. . Int J Syst Evol Microbiol 52:, 1559–1567. [CrossRef][PubMed]
    [Google Scholar]
  13. Saha R., Spröer C., Beck B., Bagley S.. ( 2010;). Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of Pseudomonas oleovorans ATCC 8062T. . Curr Microbiol 60:, 294–300. [CrossRef][PubMed]
    [Google Scholar]
  14. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. . Newark, Delaware:: Microbial ID;.
  15. Tamaoka J., Katayamafujimura Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high-performance liquid-chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  19. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  20. Xin Y. H., Zhang D. C., Liu H. C., Zhou H. L., Zhou Y. G.. ( 2009;). Pseudomonas tuomuerensis sp. nov., isolated from a bird’s nest. . Int J Syst Evol Microbiol 59:, 139–143. [CrossRef][PubMed]
    [Google Scholar]
  21. Yamamoto S., Harayama S.. ( 1995;). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. . Appl Environ Microbiol 61:, 1104–1109.[PubMed]
    [Google Scholar]
  22. Yamamoto S., Kasai H., Arnold D. L., Jackson R. W., Vivian A., Harayama S.. ( 2000;). Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. . Microbiology 146:, 2385–2394.[PubMed]
    [Google Scholar]
  23. Yuan Y., Zhou S. G., Zhuang L.. ( 2010;). Polypyrrole/carbon black composite as a novel oxygen reduction catalyst for microbial fuel cells. . J Power Sources 195:, 3490–3493. [CrossRef]
    [Google Scholar]
  24. Yumoto I., Yamazaki K., Hishinuma M., Nodasaka Y., Suemori A., Nakajima K., Inoue N., Kawasaki K.. ( 2001;). Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. . Int J Syst Evol Microbiol 51:, 349–355.[PubMed]
    [Google Scholar]
  25. Zhang L., Wang Y., Dai J., Tang Y. L., Yang Q., Luo X. S., Fang C. X.. ( 2009;). Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. . Int J Syst Evol Microbiol 59:, 1787–1792. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.054676-0
Loading
/content/journal/ijsem/10.1099/ijs.0.054676-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error