1887

Abstract

An aerobic, Gram-stain-negative, rod-shaped, DDT-resistant bacterium, designated strain CC-ALB-2, was isolated from the rhizosphere. Strain CC-ALB-2 was able to grow at 25–37 °C, at pH 5.0–8.0, with 1.0 % (w/v) NaCl and tolerate up to 200 mg l DDT. 16S rRNA gene sequence analysis of strain CC-ALB-2 showed highest sequence similarity to KCTC 2891 (97.1 %) and DSM 22821 (96.8 %), and lower levels of similarity (<97.0 %) to other species of the genus . The major fatty acid profile consisted of C 2-OH (13.1 %), C (10.0 %), C iso 3-OH (5.8 %), Cω7/Cω6 (summed feature 3, 24.7 %) and Cω7/Cω6 (summed feature 8, 42.4 %). The polar lipid profile constitutes sphingoglycolipid, glycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylglycerol and phosphatidylcholine. The polyamine pattern showed a predominance of spermidine as the major polyamine. The predominant quinone system was ubiquinone (Q-10). The DNA G+C content was 68.9±0.1 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-ALB-2 is proposed to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is CC-ALB-2 ( = BCRC 80571 = JCM 18896).

Funding
This study was supported by the:
  • National Science Council
  • Council of Agriculture, Executive Yuan
  • Ministry of Education, Taiwan, ROC
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2014-02-01
2024-11-05
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