1887

Abstract

Two bacterial strains (JC167 and JC168) were isolated from a soil sample collected from Mandpam, Tamilnadu, India. Colonies of both strains were orange and cells Gram-stain-positive. Cells were small rods, and formed terminal endospores of ellipsoidal to oval shape. Both strains were positive for catalase, oxidase and hydrolysis of starch/gelatin, and negative for chitin hydrolysis, HS production, indole production and nitrate reduction activity. Major fatty acids of both strains (>5 %) were anteiso-C, iso-C, iso-C, anteiso-C, iso-C and C with minor (<5 but >1 %) amounts of iso-C, anteiso-C B/iso-C I and Cω11. Diphosphatydilglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell wall amino acids were -alanine, -alanine, -glutamic acid and -diaminopimelic acid. β-Carotene and five unidentified carotenoids were present in both strains. Mean genomic DNA G+C content was 53.4±1 mol% and the two strains were closely related (mean DNA–DNA hybridization >90 %). 16S rRNA gene sequence comparisons of both strains indicated that they represent species of the genus within the family of the phylum . Both strains had a sequence similarity of 97.6 % with 6AG and <96.8 % with other members of the genus . Sequence similarity between strain JC167 and 168 was 100 %. Strain JC167 showed 25.8±1 % reassociation (based on DNA–DNA hybridization) with DSM 15402 ( = 6AG). Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC167 as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC167 ( = KCTC 33100 = LMG 27257).

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2014-05-01
2019-10-19
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