1887

Abstract

Three strains (14A-2-7, 14A-3-1 and 14A-3) of Gram-stain-negative, prosthecate, motile bacteria were isolated from an algal medium supplemented with 10 mg ampicillin l (w/v), in which the red alga had been cultured. Based on the 16S rRNA gene sequence analysis, the three isolates formed a cluster with the genus of the family . The sequences of the three isolates had high similarity with those of 0C-2-2 (97.6 % similarity) and G5 (95.6 % similarity). The DNA G+C contents of the three isolates ranged from 54.3 to 55.0 mol%, which were more similar to that of 0C-2-2 (58.5 mol%) than to that of G5 (47.1 mol%). The DNA–DNA relatedness showed that the three isolates were representatives of the same species (88.1–94.0 % relatedness) and that strain 14A-2-7 was a representative of a different species from 0C-2-2 and G5 (1.2–8.6 % relatedness). The phenotypic characteristics of strain 14A-2-7 differed by 20 results and 30 results from 0C-2-2 and G5, respectively. The three isolates contained ubiquinone-10 as the predominant quinone and Cω7 as the major fatty acid. Based on the polyphasic taxonomic analysis, the three isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 14A-2-7 ( = LMG 26421 = NBRC 108219). An emended description of the genus is also proposed.

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2013-12-01
2019-10-21
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References

  1. Abraham W.-R. , Lünsdorf H. , Vancanneyt M. , Smit J. . ( 2013; ). Cauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae . . Int J Syst Evol Microbiol 63:, 2207–2215. [CrossRef] [PubMed]
    [Google Scholar]
  2. Akagawa-Matsushita M. , Itoh T. , Katayama Y. , Kuraishi H. , Yamasato K. . ( 1992; ). Isoprenoid quinone composition of some marine Alteromonas, Marinomonas, Deleya, Pseudomonas and Shewanella species. . J Gen Microbiol 138:, 2275–2281. [CrossRef]
    [Google Scholar]
  3. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  4. Biebl H. , Allgaier M. , Tindall B. J. , Koblizek M. , Lünsdorf H. , Pukall R. , Wagner-Döbler I. . ( 2005; ). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. . Int J Syst Evol Microbiol 55:, 1089–1096. [CrossRef] [PubMed]
    [Google Scholar]
  5. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Fukui Y. , Abe M. , Kobayashi M. , Saito H. , Oikawa H. , Yano Y. , Satomi M. . ( 2013; ). Algimonas porphyrae gen. nov., sp. nov., a member of the family Hyphomonadaceae, isolated from the red alga Porphyra yezoensis . . Int J Syst Evol Microbiol 63:, 314–320. [CrossRef] [PubMed]
    [Google Scholar]
  7. Ikemoto S. , Katoh K. , Komagata K. . ( 1978; ). Cellular fatty acid composition in methanol-utilizing bacteria. . J Gen Appl Microbiol 24:, 41–49. [CrossRef]
    [Google Scholar]
  8. Johnson J. L. . ( 1981; ). Genetic characterization. . In Manual of Methods for General Bacteriology, pp. 450–472. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. B. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  10. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  11. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . New York:: Wiley;.
    [Google Scholar]
  12. Lee K.-B. , Liu C.-T. , Anzai Y. , Kim H. , Aono T. , Oyaizu H. . ( 2005; ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55:, 1907–1919. [CrossRef] [PubMed]
    [Google Scholar]
  13. Luthria D. L. , Sprecher H. . ( 1993; ). 2-Alkenyl-4,4-dimethyloxazolines as derivatives for the structural elucidation of isomeric unsaturated fatty acids. . Lipids 28:, 561–564. [CrossRef] [PubMed]
    [Google Scholar]
  14. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  15. Satomi M. , Kimura B. , Mizoi M. , Sato T. , Fujii T. . ( 1997; ). Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. . Int J Syst Bacteriol 47:, 832–836. [CrossRef] [PubMed]
    [Google Scholar]
  16. Sawabe T. , Oda Y. , Shiomi Y. , Ezura Y. . ( 1995; ). Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. . Microb Ecol 30:, 193–202. [CrossRef]
    [Google Scholar]
  17. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  19. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  20. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  21. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  22. Zhang X.-Y. , Li G.-W. , Wang C.-S. , Zhang Y.-J. , Xu X.-W. , Li H. , Liu A. , Liu C. , Xie B.-B. . & other authors ( 2013; ). Marinicauda pacifica gen. nov., sp. nov., a prosthecate alphaproteobacterium of the family Hyphomonadaceae isolated from deep seawater. . Int J Syst Evol Microbiol 63:, 2248–2253. [CrossRef] [PubMed]
    [Google Scholar]
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