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Volume 64, Issue Pt_1

gen. nov., sp. nov. and sp. nov., aerobic, thermophilic, long bacilli isolated from hot springs Free

Abstract

Two novel endospore-forming, aerobic bacilli, strains E173a and E265, were isolated from soil and sediment samples from Kozakli and Altinsu hot springs, Nevsehir (Turkey). Their young cells in the exponential phase of growth were motile, Gram-stain-positive, straight rods, 0.6–1.1×3.0–8.0 µm in size, but they became strikingly long, approximately 0.6–1.2 by 9.0–35.0 µm, after the stationary phase of growth. Cells varied in tests for oxidase, and had a weakly positive reaction for catalase. Both strains could grow between 40 and 70 °C, with optimal growth at 60 °C (E173a) and 55 °C (E265). Growth occurred within the range pH 5.0–11.0 with optimal growth at pH 9.0 (E173a) and pH 8.5 (E265). Strain E173a grew within a salinity range from 0 to1.5 % (w/v) NaCl with optimal growth at 0.5 %, while strain E265 grew within the range 0–5.0 % (w/v), with an optimum at 3.0 %. The new isolates differed from each other in some phenotypic and chemotaxonomic characters as well as repetitive extragenic palindromic element PCR (rep-PCR) fingerprints. 16S rRNA gene sequence similarities suggested distant relationships with other members of the family (<95.8 %), although the two strains showed 97.5 % sequence similarity between them, and had 55 % relatedness by DNA–DNA hybridization. The DNA G+C contents were 44.8 (E173a) and 43.5 mol% (E265). Moreover, the chemotaxonomic data of E173a and E265 [presence of low amounts of -diaminopimelic acid, A1γ to A1γ′ cross-linkage types in peptidoglycan, fatty acids including iso-C (>60 %), iso-C and C] supported the consideration of these isolates as members of a novel genus. Based upon phenotypic, phylogenetic and chemotaxonomic characteristics, it is proposed that new isolates represent a novel genus, gen. nov., with two novel species: sp. nov. (type strain E265 = DSM 24979 = NCIMB 14850) and sp. nov. (type strain E173a = DSM 24978 = NCIMB 14849).

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Funding
This study was supported by the:
  • Scientific Research Project Office of Ankara University (Award 11B4240003)
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2014-01-01
2024-04-26
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Thermolongibacillus altinsuensis gen. nov., sp. nov. and Thermolongibacillus kozakliensis sp. nov., aerobic, thermophilic, long bacilli isolated from hot springs
Int J Syst Evol Microbiol 64, 187 (2014); https://doi.org/10.1099/ijs.0.053280-0
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