1887

Abstract

Two novel endospore-forming, aerobic bacilli, strains E173a and E265, were isolated from soil and sediment samples from Kozakli and Altinsu hot springs, Nevsehir (Turkey). Their young cells in the exponential phase of growth were motile, Gram-stain-positive, straight rods, 0.6–1.1×3.0–8.0 µm in size, but they became strikingly long, approximately 0.6–1.2 by 9.0–35.0 µm, after the stationary phase of growth. Cells varied in tests for oxidase, and had a weakly positive reaction for catalase. Both strains could grow between 40 and 70 °C, with optimal growth at 60 °C (E173a) and 55 °C (E265). Growth occurred within the range pH 5.0–11.0 with optimal growth at pH 9.0 (E173a) and pH 8.5 (E265). Strain E173a grew within a salinity range from 0 to1.5 % (w/v) NaCl with optimal growth at 0.5 %, while strain E265 grew within the range 0–5.0 % (w/v), with an optimum at 3.0 %. The new isolates differed from each other in some phenotypic and chemotaxonomic characters as well as repetitive extragenic palindromic element PCR (rep-PCR) fingerprints. 16S rRNA gene sequence similarities suggested distant relationships with other members of the family (<95.8 %), although the two strains showed 97.5 % sequence similarity between them, and had 55 % relatedness by DNA–DNA hybridization. The DNA G+C contents were 44.8 (E173a) and 43.5 mol% (E265). Moreover, the chemotaxonomic data of E173a and E265 [presence of low amounts of -diaminopimelic acid, A1γ to A1γ′ cross-linkage types in peptidoglycan, fatty acids including iso-C (>60 %), iso-C and C] supported the consideration of these isolates as members of a novel genus. Based upon phenotypic, phylogenetic and chemotaxonomic characteristics, it is proposed that new isolates represent a novel genus, gen. nov., with two novel species: sp. nov. (type strain E265 = DSM 24979 = NCIMB 14850) and sp. nov. (type strain E173a = DSM 24978 = NCIMB 14849).

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2014-01-01
2019-10-16
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References

  1. Adkins J. P. , Cornell L. A. , Tanner R. S. . ( 1992; ). Microbial composition of carbonate petroleum reservoir fluids. . Geomicrobiol J 10:, 87–97. [CrossRef]
    [Google Scholar]
  2. Anderson D. G. , McKay L. L. . ( 1983; ). Simple and rapid method for isolating large plasmid DNA from lactic streptococci. . Appl Environ Microbiol 46:, 549–552.[PubMed]
    [Google Scholar]
  3. Ash C. , Farrow J. A. E. , Wallbanks S. , Collins M. D. . ( 1991; ). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. . Lett Appl Microbiol 13:, 202–206. [CrossRef]
    [Google Scholar]
  4. Bligh E. G. , Dyer W. J. . ( 1959; ). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37:, 911–917. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  6. Castro-Ochoa L. D. , Rodr'iguez-G'omez C. , Valerio-Alfaro G. , Oliart Ros R. . ( 2005; ). Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. . Enzyme Microb Technol 37:, 648–654. [CrossRef]
    [Google Scholar]
  7. Cihan A. C. , Ozcan B. , Tekin N. , Cokmus C. . ( 2011; ). Phylogenetic diversity of isolates belonging to genera Geobacillus and Aeribacillus isolated from different geothermal regions of Turkey. . World J Microbiol Biotechnol 27:, 2683–2696. [CrossRef]
    [Google Scholar]
  8. Cihan A. C. , Tekin N. , Ozcan B. , Cokmus C. . ( 2012; ). The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of turkey. . Braz J Microbiol 43:, 309–324. [CrossRef] [PubMed]
    [Google Scholar]
  9. Claus D. , Berkeley C. W. . ( 1986; ). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by Sneath P. H. A. , Mair N. S. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  10. Cohn F. . ( 1872; ). Untersuchungen über Bakterien. . Beitr Biol Pflanz 1:, 127–224 (in German).
    [Google Scholar]
  11. Coleri A. , Cokmus C. , Ozcan B. , Akkoc N. , Akcelik M. . ( 2009; ). Isolation of α-glucosidase-producing thermophilic bacilli from hot springs of Turkey. . Microbiology (English translation of Mikrobiologiia) 78:, 56–66. [CrossRef]
    [Google Scholar]
  12. Coorevits A. , Dinsdale A. E. , Halket G. , Lebbe L. , De Vos P. , Van Landschoot A. , Logan N. A. . ( 2012; ). Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly ‘thermoglucosidasius’); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.. Int J Syst Evol Microbiol 62:, 1470–1485. [CrossRef] [PubMed]
    [Google Scholar]
  13. Daffonchio D. , Cherif A. , Brusetti L. , Rizzi A. , Mora D. , Boudabous A. , Borin S. . ( 2003; ). Nature of polymorphisms in 16S-23S rRNA gene intergenic transcribed spacer fingerprinting of Bacillus and related genera. . Appl Environ Microbiol 69:, 5128–5137. [CrossRef] [PubMed]
    [Google Scholar]
  14. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  15. Denizci A. A. , Kazan D. , Abeln E. C. A. , Erarslan A. . ( 2004; ). Newly isolated Bacillus clausii GMBAE 42: an alkaline protease producer capable to grow under highly alkaline conditions. . J Appl Microbiol 96:, 320–327. [CrossRef] [PubMed]
    [Google Scholar]
  16. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  17. Fortina M. G. , Pukall R. , Schumann P. , Mora D. , Parini C. , Manachini P. L. , Stackebrandt E. . ( 2001; ). Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.. Int J Syst Evol Microbiol 51:, 447–455.[PubMed] [CrossRef]
    [Google Scholar]
  18. Fritze D. . ( 2004; ). Taxonomy of the genus Bacillus and related genera: the aerobic endospore-forming bacteria. . Phytopathology 94:, 1245–1248. [CrossRef] [PubMed]
    [Google Scholar]
  19. Gessesse A. , Gashe B. A. . ( 1997; ). Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake. . Biotechnol Lett 19:, 479–481. [CrossRef]
    [Google Scholar]
  20. Golovacheva R. S. , Karavaiko G. . ( 1978; ). Sulfobacillus, a new genus of thermophilic sporeforming bacteria. . Microbiologiia 47:, 815–822 (in Russian).
    [Google Scholar]
  21. Halvorson H. . ( 1966; ). α-Glucosidase from yeast. . Methods Enzymol 8:, 559–562. [CrossRef]
    [Google Scholar]
  22. Huss V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef] [PubMed]
    [Google Scholar]
  23. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  24. Kuykendall L. D. , Roy M. A. , O'Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  25. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . New York:: Wiley;.
    [Google Scholar]
  26. Lee D. W. , Koh Y. S. , Kim K. J. , Kim B. C. , Choi H. J. , Kim D. S. , Suhartono M. T. , Pyun Y. . ( 1999; ). Isolation and characterization of a thermophilic lipase from bacillus thermoleovorans ID-1. . FEMS Microbiol Lett 179:, 393–400. [CrossRef] [PubMed]
    [Google Scholar]
  27. Logan N. A. , Berge O. , Bishop A. H. , Busse H.-J. , De Vos P. , Fritze D. , Heyndrickx M. , Kämpfer P. , Rabinovitch L. . & other authors ( 2009; ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef] [PubMed]
    [Google Scholar]
  28. Mesbah M. , Premachandran U. , Whitman W. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  29. Miller L. T. . ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  30. Miñana-Galbis D. , Pinzón D. L. , Lorén J. G. , Manresa A. , Oliart-Ros R. M. . ( 2010; ). Reclassification of Geobacillus pallidus (Scholz et al. 1988) Banat et al. 2004 as Aeribacillus pallidus gen. nov., comb. nov.. Int J Syst Evol Microbiol 60:, 1600–1604. [CrossRef] [PubMed]
    [Google Scholar]
  31. Nakamura K. , Haruta S. , Ueno S. , Ishii M. , Yokota A. , Igarashi Y. . ( 2004; ). Cerasibacillus quisquiliarum gen. nov., sp. nov., isolated from a semi-continuous decomposing system of kitchen refuse. . Int J Syst Evol Microbiol 54:, 1063–1069. [CrossRef] [PubMed]
    [Google Scholar]
  32. Nazina T. N. , Tourova T. P. , Poltaraus A. B. , Novikova E. V. , Grigoryan A. A. , Ivanova A. E. , Lysenko A. M. , Petrunyaka V. V. , Osipov G. A. . & other authors ( 2001; ). Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans . . Int J Syst Evol Microbiol 51:, 433–446.[PubMed]
    [Google Scholar]
  33. Pikuta E. , Lysenko A. , Chuvilskaya N. , Mendrock U. , Hippe H. , Suzina N. , Nikitin D. , Osipov G. , Laurinavichius K. . ( 2000; ). Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb. nov.. Int J Syst Evol Microbiol 50:, 2109–2117. [CrossRef] [PubMed]
    [Google Scholar]
  34. Rainey F. A. , Fritze D. , Stackebrandt E. . ( 1994; ). The phylogenetic diversity of thermophilic members of the genus Bacillus as revealed by 16S rDNA analysis. . FEMS Microbiol Lett 115:, 205–211. [CrossRef] [PubMed]
    [Google Scholar]
  35. Rhuland L. E. , Work E. , Denman R. F. , Hoare D. S. . ( 1955; ). The behaviour of the isomers of 2,6-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77:, 4844–4846. [CrossRef]
    [Google Scholar]
  36. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  37. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  38. Shida O. , Takagi H. , Kadowaki K. , Komagata K. . ( 1996; ). Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.. Int J Syst Bacteriol 46:, 939–946. [CrossRef] [PubMed]
    [Google Scholar]
  39. Stackebrandt E. , Koch C. , Gvozdiak O. , Schumann P. . ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend.. Int J Syst Bacteriol 45:, 682–692. [CrossRef] [PubMed]
    [Google Scholar]
  40. Stackebrandt E. , Rainey F. A. , Ward-Rainey N. L. . ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:, 479–491. [CrossRef]
    [Google Scholar]
  41. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. J. , Nesme X. , Rosselló-Mora R. , Swings J. . & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  42. Suzuki Y. , Kishigami T. , Abe S. . ( 1976; ). Production of extracellular α-glucosidase by a thermophilic Bacillus species. . Appl Environ Microbiol 31:, 807–812.[PubMed]
    [Google Scholar]
  43. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  44. Tindall B. J. . ( 1990a; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  45. Tindall B. J. . ( 1990b; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  46. Tindall B. J. , Sikorski J. , Smibert R. M. , Kreig N. R. . ( 2007; ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M. . . Washington, DC,:: American Society for Microbiology;.
    [Google Scholar]
  47. Touzel J. P. , O’Donohue M. , Debeire P. , Samain E. , Breton C. . ( 2000; ). Thermobacillus xylanilyticus gen. nov., sp. nov., a new aerobic thermophilic xylan-degrading bacterium isolated from farm soil. . Int J Syst Evol Microbiol 50:, 315–320. [CrossRef] [PubMed]
    [Google Scholar]
  48. Versalovic J. , Schneider M. , de Bruijn F. J. , Lupski J. R. . ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). . Methods Mol Cell Biol 5:, 25–40.
    [Google Scholar]
  49. Wisotzkey J. D. , Jurtshuk P. Jr , Fox G. E. , Deinhard G. , Poralla K. . ( 1992; ). Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov.. Int J Syst Bacteriol 42:, 263–269. [CrossRef] [PubMed]
    [Google Scholar]
  50. Xue Y. , Zhang X. , Zhou C. , Zhao Y. , Cowan D. A. , Heaphy S. , Grant W. D. , Jones B. E. , Ventosa A. , Ma Y. . ( 2006; ). Caldalkalibacillus thermarum gen. nov., sp. nov., a novel alkalithermophilic bacterium from a hot spring in China. . Int J Syst Evol Microbiol 56:, 1217–1221. [CrossRef] [PubMed]
    [Google Scholar]
  51. Zhao W. , Weber C. , Zhang C. L. , Romanek C. S. , King G. M. , Mills G. , Sokolova T. , Wiegel J. . ( 2006; ). Thermalkalibacillus uzonensis gen. nov. sp. nov, a novel aerobic alkali-tolerant thermophilic bacterium isolated from a hot spring in Uzon Caldera, Kamchatka. . Extremophiles 10:, 337–345. [CrossRef] [PubMed]
    [Google Scholar]
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