1887

Abstract

A taxonomic study was performed on Gram-stain-positive, catalase-negative and regular rod-shaped bacterial strains R4B and R4C, isolated from the stomachs of honeybees. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strains was within the genus ; the highest sequence similarity to R4B was shown by BCRC 10695 (93.6 %). Lower sequence similarities were found to other obligately homofermentative lactobacilli. A PCR–DGGE method could detect the sequence of the 16S rRNA gene of strain R4B at different developmental stages of honeybees occurring in two different locations in the Czech Republic. The distinctiveness of the strains from other lactobacilli was also confirmed by analysis of sequences of other phylogenetic markers applicable to the taxonomy of the genus , ribotyping and rep-PCR analysis. The DNA G+C content of strain R4B was 41.3 mol%. The predominant cellular fatty acids of strain R4B were Cω9, summed Cω6/C cyclo ω10, C, summed Cω7/Cω6 and summed Cω7/Cω6. The major polar lipids of strain R4B were glycolipids, lipids and phospholipids. Phenotypic and phylogenetic characteristics also confirmed the independent status of the strains at the species level. Interestingly, strain R4B was able to inhibit growth of subsp. (causal agent of American foulbrood in honeybees) and (causal agent of European foulbrood). The name sp. nov. is proposed for this novel taxon; the type strain is R4B ( = CCM 8403 = LMG 26964).

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2014-01-01
2019-11-17
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