1887

Abstract

Two Gram-staining-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated and non-spore-forming amylolytic marine bacterial strains, designated CC-AMZ-30M and CC-AMZ-30N, were isolated from coastal surface seawater in Taiwan. Strain CC-AMZ-30M shared pairwise 16S rRNA gene sequence similarities of 95.8, 95.0 and <94.0 % to CC-TBT-3, CC-AMO-30B and other sphingomonads, respectively. Strain CC-AMZ-30N shared 97.0, 96.7, 95.0 and <95.1 % similarities to strain CC-AMZ-30M, CC-TBT-3, CC-AMO-30B and other sphingomonads, respectively. The common polar lipids of the two strains include a signature glycolipid (GL2), diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingoglycolipid in major amounts besides moderate-to-trace amounts of an unidentified aminolipid and several unidentified glycolipids. Both strains contained Cω7/Cω6, Cω7/Cω6, C and C 2-OH as major (>5 % of the total) fatty acids. Strains CC-AMZ-30M and CC-AMZ-30N had DNA G+C contents of 64.2 and 65.2 mol%, respectively. The major polyamine was spermidine in strain CC-AMZ-30M and triamine -homospermidine in strain CC-AMZ-30N. Both strains contained ubiquinone Q-10 as the predominant respiratory quinone. Differential phylogenetic and chemotaxonomic evidence including the presence of characteristic GL2, C 2-OH and several other phenotypic features supported the classification of strains CC-AMZ-30M and CC-AMZ-30N as two novel species of the genus , for which we propose the names sp. nov. and sp. nov., respectively; corresponding type strains are CC-AMZ-30M ( = JCM 18554 = BCRC 80466) and CC-AMZ-30N ( = JCM 18555 = BCRC 80467). An emended description of the genus is also proposed.

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2013-12-01
2019-10-22
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