1887

Abstract

Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 -like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average nucleotide identity (ANIb) of less than 85 % to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus ; (i) a clade representing , , , , and , which we refer to as , (ii) a clade consisting of and two newly described species, sp. nov. (type strain FSL S10-1188 = DSM 26686 = LMG 28120 = BEI NR-42633) and sp. nov. (type strain FSL S10-1187 = DSM 26687 = LMG 28121 = BEI NR-42632), (iii) a clade consisting of , and three novel species, sp. nov. (type strain TTU A1-0210 = FSL F6-0969 = DSM 26689 = LMG 28123 = BEI NR-42630), sp. nov. (type strain TTU A1-0212 = FSL F6-0971 = DSM 26688 = LMG 28122 = BEI NR-42631) and sp. nov. (type strain FSL S10-1204 = DSM 26685 = LMG 28119 = BEI NR- 42634) and (iv) a clade containing . Genomic and phenotypic data suggest that the novel species are non-pathogenic.

Funding
This study was supported by the:
  • , USDA NIFA , (Award Special Research Grant 2010-34459-20756)
  • , USDA NIFA National Integrated Food Safety Initiative , (Award 2008-51110-04688 and 2008-51110-04333)
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2014-06-01
2020-11-30
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