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Volume 64, Issue Pt_2

gen. nov., sp. nov., a psychrotolerant bacterium of the family , isolated from glacier ice water Free

Abstract

A Gram-stain-positive, aerobic, non-spore-forming, short-rod-shaped bacterium, designated strain MP203, was isolated from ice water of Midui Glacier in Tibet Autonomous Region, China. The strain was psychrotolerant, growing at 0–25 °C. 16S rRNA gene sequence analysis showed that strain MP203 was most similar to NBRC 103066, KACC 13354, KCTC 19311, CCTCC AB 209077 and JCM 18951, with similarities of 97.4, 97.2, 97.2, 97.1 and 97.1 %, respectively. The maximum-likelihood phylogenetic tree indicated that strain MP203 clustered with nine genera of the family , namely , , , , , , , and However, bootstrap analysis showed that there was no significance in the branching pattern of the linage comprising strain MP203 and any existing generic lineage of the family . DNA–DNA hybridization results indicated levels of relatedness between strain MP203 and CCTCC AB 209077, NBRC 103066, KCTC 19311, KACC 13354 and JCM 18951 were 25.8±7.3, 29.6±7.6, 19.7±6.7, 16.0±4.2 and 12.4±5.1 % (mean±), respectively. The G+C content of the genomic DNA was 64.1 mol%. Analysis of the cell-wall peptidoglycan revealed that the peptidoglycan structure of strain MP203 was B10 type with Gly[-Hse]–-Glu–-DAB, containing 2, 4-diaminobutyric acid (DAB) as a diagnostic amino acid. The cell-wall sugars were rhamnose, ribose, mannose and glucose. The major fatty acids were anteiso-C, iso-C and anteiso A-C. An unusual compound identified as anteiso-C-DMA (1, 1-dimethoxy-anteiso-pentadecane) was also present in strain MP203. The predominant menaquinone was MK-10. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unknown glycolipid and four unknown lipids were detected in the polar lipid extracts. As strain MP203 was distinguishable from phylogenetically related genera in the family in terms of its physiological and chemotaxonomic characteristics and phylogenetic position, it was considered to represent a novel species of a new genus. Thus, the name gen. nov., sp. nov. is proposed. The type strain of is MP203 ( = CGMCC 1.12484 = KCTC 29148).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (NSFC (Award 187 31070001)
  • Knowledge Innovation Program of Chinese Academy of Sciences (Award 188 KSCX2-YW-Z-0937)
  • KRIBB Research Initiative Program
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2014-02-01
2024-04-26
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Glaciihabitans tibetensis gen. nov., sp. nov., a psychrotolerant bacterium of the family Microbacteriaceae, isolated from glacier ice water
Int J Syst Evol Microbiol 64, 579 (2014); https://doi.org/10.1099/ijs.0.052670-0
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