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Abstract

A rod-shaped actinobacterium, designated strain RS-16, was isolated from a rhizosphere soil and its taxonomic position was determined by a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis indicated that strain RS-16 was most closely related to the type strain of (98.3 % sequence similarity). The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid and -glycolylated murein. The major whole-cell sugars were glucose, galactose and mannose. The predominant menaquinone was MK-9(H). The major fatty acids were Cω9 and C. The DNA G+C content was 73.1 mol%. The phenotypic and DNA–DNA hybridization data showed that strain RS-16 ( = KACC 16209 = DSM 45622) represents a novel species of the genus , for which sp. nov. is proposed. Based on the phylogenetic position determined by 16S rRNA gene analysis and the signature nucleotide set of the 16S rRNA sequence, the genus represents a novel family of the suborder , for which the name fam. nov. is proposed.

Funding
This study was supported by the:
  • , 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology, Republic of Korea
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2013-10-01
2021-01-24
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