1887

Abstract

A novel actinobacterium, designated strain NJ2035, was isolated from soil collected from Abuja, Nigeria and was characterized to determine its taxonomic position. The isolate was found to have chemical and morphological properties associated with members of the genus . Phylogenetic analyses based on almost-complete 16S rRNA gene sequences indicated that the isolate was closely related to members of the genus , and was shown to form a distinct phyletic line in the phylogenetic tree. Strain NJ2035 was most closely related to C81, C61 and C68 (98.5 % 16S rRNA gene sequence similarity). Sequence similarities with other members of the genus were less than 98.2 %. The cell wall of the novel strain contained -diaminopimelic acid, and galactose, mannose and rhamnose as the diagnostic sugars. The predominant menaquinone was MK-9(H). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. DNA–DNA relatedness and phenotypic data showed that the novel isolate and C81, C61 and C68 belong to distinct genomic species. On the basis of data from this taxonomic study using a polyphasic approach, strain NJ2035 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NJ2035 ( = DSM 45680 = KCTC 29057 = NRRL B-24881).

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2013-10-01
2019-10-23
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