1887

Abstract

Two Gram-reaction-negative, rod-shaped, non-motile and red–pink-pigmented bacterial strains, designated GYR3077 and GSR0100, were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077 and GSR0100 exhibited sequence similarities of 95.9 % to ZLB-3 and 96.6 % to PB17, respectively, and indicated that these isolates belonged to the phylum . The major cellular fatty acids present in the two isolates were iso-C, Cω5, summed feature 4 (iso-C I and/or anteiso-C B) and summed feature 3 (Cω7 and/or Cω6). The major respiratory quinone and polyamine patterns were menaquinone-7 and -homospermidine, characteristic of the genus . Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077 and GSR0100 were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077 and GSR0100 was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077 and GSR0100 were considered to represent two novel species of the genus , for which the names sp. nov. (type strain GYR3077 = KACC 16451 = JCM 17924) and sp. nov. (type strain GSR0100 = KACC 16452 = JCM 17923) are proposed.

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2013-12-01
2019-10-23
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References

  1. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  3. Buczolits S., Denner E. B. M., Kämpfer P., Busse H.-J.. ( 2006;). Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch et al. 1999. . Int J Syst Evol Microbiol 56:, 2071–2078. [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  5. Collins M. D., Hutson R. A., Grant I. R., Patterson M. F.. ( 2000;). Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov.. Int J Syst Evol Microbiol 50:, 731–734. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Hirsch P., Ludwig W., Hethke C., Sittig M., Hoffmann B., Gallikowski C. A.. ( 1998;). Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antartica soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. . Syst Appl Microbiol 21:, 374–383. [CrossRef][PubMed]
    [Google Scholar]
  10. Hoang V.-A., Kim Y.-J., Nguyen N. L., Yang D.-C.. ( 2013;). Hymenobacter ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 63:, 661–666. [CrossRef][PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  14. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  15. Scherer P., Kneifel H.. ( 1983;). Distribution of polyamines in methanogenic bacteria. . J Bacteriol 154:, 1315–1322.[PubMed]
    [Google Scholar]
  16. Tamaoka J., Katayama-Fujimura Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Microbiol 54:, 31–36. [CrossRef]
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  19. Tittsler R. P., Sandholzer L. A.. ( 1936;). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31:, 575–580.[PubMed]
    [Google Scholar]
  20. Zhang Q., Liu C., Tang Y., Zhou G., Shen P., Fang C., Yokota A.. ( 2007;). Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 57:, 1752–1756. [CrossRef][PubMed]
    [Google Scholar]
  21. Zhang L., Dai J., Tang Y., Luo X., Wang Y., An H., Fang C., Zhang C.. ( 2009;). Hymenobacter deserti sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 59:, 77–82. [CrossRef][PubMed]
    [Google Scholar]
  22. Zhang D. C., Busse H. J., Liu H. C., Zhou Y. G., Schinner F., Margesin R.. ( 2011;). Hymenobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from soil. . Int J Syst Evol Microbiol 61:, 859–863. [CrossRef][PubMed]
    [Google Scholar]
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