1887

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped, non-motile and yellow-pigmented bacterial strain, designated KMM 6208, was isolated from a sea urchin. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that this novel isolate was affiliated to the class and formed a robust cluster with KMM 3895 with 98.2 % 16S rRNA gene sequence similarity. Strain KMM 6208 grew in the presence of 0.5–5 % NaCl and at a temperature range of 4–38 °C. The isolate was oxidase-positive and hydrolysed aesculin, casein, chitin, gelatin, starch and Tweens 40 and 80. The prevalent fatty acids of strain KMM 6208 were Cω7, iso-C iso-C Cω7 and C The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid, and the major isoprenoid quinone was Q-8. The DNA G+C content of strain KMM 6208 was 46.3 mol%. The DNA–DNA relatedness value of strain KMM 6208 with KMM 3895 was 5 %. Molecular data in a combination with phenotypic findings strongly suggest inclusion of this novel strain in the genus as a representative of a novel species for which the name sp. nov. is proposed. The type strain is KMM 6208 ( = KCTC 12711 = LMG 26983).

Funding
This study was supported by the:
  • , Presidium of the Russian Academy of Sciences ‘Molecular and Cell Biology’
  • , Presidium of the Far-Eastern Branch of the Russian Academy of Sciences , (Award 12-III-A-06-105)
  • , government of the Russian Federation , (Award 11.G34.31.0010)
  • , Russian Foundation for Basic Research (RFBR) , (Award 11-04-00781)
  • , Federal Public Planning Service – Science Policy
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051599-0
2013-11-01
2020-07-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4124.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051599-0&mimeType=html&fmt=ahah

References

  1. Balakirev E. S., Krupnova T. N., Ayala F. J. ( 2012 ). Symbiotic associations in the phenotypically-diverse brown alga Saccharina japonica . . PLoS ONE 7, e39587. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bligh E. G., Dyer W. J. ( 1959 ). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37, 911917. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cleenwerck I., Vandemeulebroecke K., Janssens D., Swings J. ( 2002 ). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.. Int J Syst Evol Microbiol 52, 15511558. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D., Shah H. M. ( 1984 ). Fatty acid, menaquinone and polar lipid composition of Rothia dentocariosa . . Arch Microbiol 137, 247249. [CrossRef]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. ( 1998 ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44, 11481153. [CrossRef]
    [Google Scholar]
  9. Komagata K., Suzuki K.-I. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  10. Lawrence J. M. (editor) ( 2007 ). Edible Sea Urchins: Biology and Ecology. (Developments in Aquaculture and Fisheries Science, vol. 37) Amsterdam:: Elsevier Science;.
    [Google Scholar]
  11. Lemos M. L., Toranzo A. E., Barja J. L. ( 1985 ). Modified medium for the oxidation-fermentation test in the identification of marine bacteria. . Appl Environ Microbiol 49, 15411543.[PubMed]
    [Google Scholar]
  12. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  13. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  14. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. ( 2010 ). Arenicella xantha gen. nov., sp. nov., a gammaproteobacterium isolated from a marine sandy sediment. . Int J Syst Evol Microbiol 60, 18321836. [CrossRef] [PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  16. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20, 16.
    [Google Scholar]
  17. Schloss P. D., Westcott S. L., Ryabin T., Hall J. R., Hartmann M., Hollister E. B., Lesniewski R. A., Oakley B. B., Parks D. H. & other authors ( 2009 ). Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. . Appl Environ Microbiol 75, 75377541. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  19. Vancanneyt M., Naser S. M., Engelbeen K., De Wachter M., Van der Meulen R., Cleenwerck I., Hoste B., De Vuyst L., Swings J. ( 2006 ). Reclassification of Lactobacillus brevis strains LMG 11494 and LMG 11984 as Lactobacillus parabrevis sp. nov.. Int J Syst Evol Microbiol 56, 15531557. [CrossRef] [PubMed]
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler P., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  21. Wilson K. ( 1987 ). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 2.4.12.4.5. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Greene Publishing and Wiley-Interscience;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051599-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051599-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error