1887

Abstract

A Gram-stain-negative, aerobic, moderately halophilic bacterium, strain DY53, was isolated from a deep-seawater sample collected from the eastern Pacific Ocean. This isolate grew in the presence of 0.5–10.0 % (w/v) NaCl, at pH 6.5–8.5 and at 15–40 °C. The optimum NaCl concentration for growth of DY53 was 2 % (w/v) at 35 °C. Chemotaxonomic analysis showed MK-7 as the predominant menaquinone and iso-C, summed feature 3 (iso-C 2-OH and/or Cω7), iso-C G, iso-C 3-OH and iso-C 3-OH as major cellular fatty acids. The genomic DNA G+C content was 40.8 mol%. Phylogenetic trees based on 16S rRNA gene sequences revealed that UST030701-097 was the closest neighbour, with 96.7 % sequence similarity. Based on phylogenetic, chemotaxonomic and phenotypic data, we propose that strain DY53 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DY53( = CGMCC 1.12402 = JCM 18885).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051276-0
2013-10-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/10/3710.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051276-0&mimeType=html&fmt=ahah

References

  1. Cha I.-T., Oh Y.-S., Park S.-J., Park B.-J., Lee J.-K., Lim C.-S., Park A.-R., Yoo J.-S., Lee D.-H.. & other authors ( 2011;). Reichenbachiella faecimaris sp. nov., isolated from a tidal flat, and emended descriptions of the genus Reichenbachiella and Reichenbachiella agariperforans. . Int J Syst Evol Microbiol 61:, 1994–1999. [CrossRef][PubMed]
    [Google Scholar]
  2. Dong X.-Z., Cai M.-Y.. ( 2001;). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press (English translation);.
    [Google Scholar]
  3. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  4. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  5. Hosoya S., Yokota A.. ( 2007;). Flammeovirga kamogawensis sp. nov., isolated from coastal seawater in Japan. . Int J Syst Evol Microbiol 57:, 1327–1330. [CrossRef][PubMed]
    [Google Scholar]
  6. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  7. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  8. Lau S. C. K., Tsoi M. M. Y., Li X., Plakhotnikova I., Dobretsov S., Wu M., Wong P.-K., Pawlik J. R., Qian P.-Y.. ( 2006;). Description of Fabibacter halotolerans gen. nov., sp. nov. and Roseivirga spongicola sp. nov., and reclassification of [Marinicola] seohaensis as Roseivirga seohaensis comb. nov.. Int J Syst Evol Microbiol 56:, 1059–1065. [CrossRef][PubMed]
    [Google Scholar]
  9. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  10. Lewin R. A.. ( 1970;). Flexithrix dorotheae gen. et sp. nov. (Flexibacterales); and suggestions for reclassifying sheathed bacteria. . Can J Microbiol 16:, 511–515. [CrossRef]
    [Google Scholar]
  11. Ludwig W., Euzéby J., Whitman W. B.. ( 2008;). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes. . http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf
    [Google Scholar]
  12. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  13. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  14. Mikhailov V. V., Romanenko L. A., Ivanova E. P.. ( 2006;). The genus Alteromonas and related proteobacteria. . In The Prokaryotes, , 3rd edn., vol. 6, pp. 597–645. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E... New York:: Springer;. [CrossRef]
    [Google Scholar]
  15. Nedashkovskaya O. I., Kim S. B., Lee D. H., Lysenko A. M., Shevchenko L. S., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S.. ( 2005;). Roseivirga ehrenbergii gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’, isolated from the green alga Ulva fenestrata. . Int J Syst Evol Microbiol 55:, 231–234. [CrossRef][PubMed]
    [Google Scholar]
  16. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Bae K. S.. ( 2010;). Reclassification of Flexibacter tractuosus (Lewin 1969) Leadbetter 1974 and ‘Microscilla sericea’ Lewin 1969 in the genus Marivirga gen. nov. as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov.. Int J Syst Evol Microbiol 60:, 1858–1863. [CrossRef][PubMed]
    [Google Scholar]
  17. Nupur S., Sharma S., Kumar Singh P., Suresh K., Anil Kumar P.. ( 2012;). Fulvivirga imtechensis sp. nov., a member of the phylum Bacteroidetes. . Int J Syst Evol Microbiol 62:, 2213–2217. [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Seo H.-S., Kwon K. K., Yang S.-H., Lee H.-S., Bae S. S., Lee J.-H., Kim S.-J.. ( 2009;). Marinoscillum gen. nov., a member of the family ‘Flexibacteraceae’, with Marinoscillum pacificum sp. nov. from a marine sponge and Marinoscillum furvescens nom. rev., comb. nov.. Int J Syst Evol Microbiol 59:, 1204–1208. [CrossRef][PubMed]
    [Google Scholar]
  20. Srisukchayakul P., Suwanachart C., Sangnoi Y., Kanjana-Opas A., Hosoya S., Yokota A., Arunpairojana V.. ( 2007;). Rapidithrix thailandica gen. nov., sp. nov., a marine gliding bacterium isolated from the Andaman sea, along the southern coastline of Thailand. . Int J Syst Evol Microbiol 57:, 2275–2279. [CrossRef][PubMed]
    [Google Scholar]
  21. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  22. Takahashi M., Suzuki K., Nakagawa Y.. ( 2006;). Emendation of the genus Flammeovirga and Flammeovirga aprica with the proposal of Flammeovirga arenaria nom. rev., comb. nov. and Flammeovirga yaeyamensis sp. nov.. Int J Syst Evol Microbiol 56:, 2095–2100. [CrossRef][PubMed]
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  25. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M.. ( 2007;). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. . Int J Syst Evol Microbiol 57:, 1619–1624. [CrossRef][PubMed]
    [Google Scholar]
  26. Xu X.-W., Huo Y.-Y., Bai X.-D., Wang C.-S., Oren A., Li S.-Y., Wu M.. ( 2011;). Kordiimonas lacus sp. nov., isolated from a ballast water tank, and emended description of the genus Kordiimonas. . Int J Syst Evol Microbiol 61:, 422–426. [CrossRef][PubMed]
    [Google Scholar]
  27. Xu H., Fu Y., Yang N., Ding Z., Lai Q., Zeng R.. ( 2012;). Flammeovirga pacifica sp. nov., isolated from deep-sea sediment. . Int J Syst Evol Microbiol 62:, 937–941. [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon J., Ishikawa S., Kasai H., Yokota A.. ( 2007;). Perexilibacter aurantiacus gen. nov., sp. nov., a novel member of the family ‘Flammeovirgaceae’ isolated from sediment. . Int J Syst Evol Microbiol 57:, 964–968. [CrossRef][PubMed]
    [Google Scholar]
  29. Yoon J., Matsuo Y., Kasai H., Yokota A.. ( 2008;). Limibacter armeniacum gen. nov., sp. nov., a novel representative of the family ‘Flammeovirgaceae’ isolated from marine sediment. . Int J Syst Evol Microbiol 58:, 982–986. [CrossRef][PubMed]
    [Google Scholar]
  30. Yoon J., Adachi K., Park S., Kasai H., Yokota A.. ( 2011;). Aureibacter tunicatorum gen. nov., sp. nov., a marine bacterium isolated from a coral reef sea squirt, and description of Flammeovirgaceae fam. nov.. Int J Syst Evol Microbiol 61:, 2342–2347. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051276-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051276-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error