1887

Abstract

A novel Gram-reaction-positive actinobacterial strain, designated CF5/3, was isolated from a sand sample obtained in the Sahara Desert, Chad. The greenish-black-pigmented isolate was aerobic and exhibited optimal growth from 25–40 °C at pH 6.0–10.0 with 0–1 % (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus . The DNA G+C content of the genome of the novel strain was 75.5 mol%. The peptidoglycan contained -diaminopimelic acid as diagnostic diamino acid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol. MK-9(H) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids: iso-C and iso-C. Analysis of 16S rRNA gene sequences showed 95.6–98.8 % pairwise sequence identity with the members of the genus . Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, , with the type strain CF5/3 ( = DSM 45417 = CCUG 62814 = MTCC 11412).

Funding
This study was supported by the:
  • European Social Fund Operational Programme
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2013-09-01
2021-10-18
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