1887

Abstract

A Gram-reaction-negative, aerobic, motile by one polar flagellum, yellow-pigmented, rod-shaped bacterium, designated strain THG-B117, was isolated from soil of a cornus fruit field of Hoengseong province in South Korea and its taxonomic position was investigated by a polyphasic study. Strain THG-B117 grew well at 25–30 °C and at pH 6.0–8.0 in the absence of NaCl on nutrient agar. On the basis of 16S rRNA gene sequence similarity, strain THG-B117 was shown to belong to the family and be related to XD53 (98.7 % similarity), JS14-6 (98.0 %), BB4 (96.9 %), JS12-10 (96.9 %) and ATSB10 (96.7 %). DNA–DNA hybridization experiments showed that DNA relatedness between strain THG-B117 and its phylogenetically closest neighbours was below 45.1 %. The G+C content of the genomic DNA of strain THG-B117 was 64.8 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unidentified aminolipids, unidentified aminophospholipids and unidentified phospholipids. Phenotypic and chemotaxonomic data (major ubiquinone was Q-8, and major fatty acids were iso-C, iso-C, iso-C and iso-Cω9) supported the affiliation of strain THG-B117 with the genus . The results of physiological and biochemical tests suggested that strain THG-B117 was different genotypically and phenotypically from recognized species of the genus , and represents a novel species of this genus. The name sp. nov. is proposed, with the type strain THG-B117 ( = KACC 16981 = JCM 18747).

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2013-10-01
2020-01-26
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