1887

Abstract

A novel halotolerant actinomycete, designated strain BNT52, was isolated from soil collected from Cihanbeyli Salt Mine in the central Anatolia region of Turkey, and examined using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the genus and formed a distinct phyletic line in the 16S rRNA gene tree. Strain BNT52 was most closely related to CSC17Ta-90 (96.7 %), KT2025 (96.6 %), DSM 46092 (96.6 %), YIM 75904 (96.5 %), RP-AC36 (96.4 %) and DSM 44468 (96.4 %). Sequence similarities with other strains of species of the genus were lower than 96.2 %. The isolate grew at 20–37 °C, pH 6–12 and in the presence of 0–10 % (w/v) NaCl. The cell wall of the novel strain contained -diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids were iso-C 2-OH and iso-C. The predominant menaquinone was MK-9(H). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylmethylethanolamine. The genomic DNA G+C content was 68.8 mol%. On the basis of the data from this polyphasic taxonomic study, strain BNT52 represents a novel species within the genus for which the name sp. nov. is proposed (type strain BNT52 = KCTC 29065 = NRRL B-24886 = DSM 45679).

Funding
This study was supported by the:
  • , Ondokuz Mayis University (OMU) , (Award no. PYO. FEN. 1901.09.003)
  • , Anadolu University (AU) , (Award no. 1201F012)
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2013-10-01
2020-07-04
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References

  1. Chun J., Goodfellow M. ( 1995 ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45, 240245. [CrossRef] [PubMed]
    [Google Scholar]
  2. Embley T. M., Smida J., Stackebrandt E. ( 1988 ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and description of Pseudonocardiaceae fam. nov.. Syst Appl Microbiol 11, 4452. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogeny: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C. ( 2005 ). A simple fluorimetric method for the estimation of DNA–DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles 9, 7579. [CrossRef] [PubMed]
    [Google Scholar]
  6. Goodfellow M., Alderson G., Lacey J. ( 1979 ). Numerical taxonomy of Actinomadura and related actinomycetes. . J Gen Microbiol 112, 95111. [CrossRef] [PubMed]
    [Google Scholar]
  7. Groth I., Tan G. Y. A., González J. M., Laiz L., Carlsohn M. R., Schütze B., Wink J., Goodfellow M. ( 2007 ). Amycolatopsis nigrescens sp. nov., an actinomycete isolated from a Roman catacomb. . Int J Syst Evol Microbiol 57, 513519. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hsu S. C., Lockwood J. L. ( 1975 ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29, 422426.[PubMed]
    [Google Scholar]
  9. Jones K. L. ( 1949 ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57, 141145.[PubMed]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [CrossRef]
    [Google Scholar]
  12. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  13. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [CrossRef]
    [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. ( 1970 ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20, 435443. [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977 ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5, 249260. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J.-S. ( 1986 ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov.. Int J Syst Bacteriol 36, 2937. [CrossRef]
    [Google Scholar]
  18. Lee S. D. ( 2006 ). Amycolatopsis jejuensis sp. nov. and Amycolatopsis halotolerans sp. nov., novel actinomycetes isolated from a natural cave. . Int J Syst Evol Microbiol 56, 549553. [CrossRef] [PubMed]
    [Google Scholar]
  19. Lee S. D. ( 2009 ). Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis . . Int J Syst Evol Microbiol 59, 14011404. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lee S. D., Kinkel L. L., Samac D. A. ( 2006 ). Amycolatopsis minnesotensis sp. nov., isolated from a prairie soil. . Int J Syst Evol Microbiol 56, 265269. [CrossRef] [PubMed]
    [Google Scholar]
  21. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin layer chromotography of methanolysates of mycolic acid-containing bacteria. . J. Chromatogr A 188, 221233. [CrossRef] [PubMed]
    [Google Scholar]
  22. Nash P., Krent M. M. ( 1991 ). Culture media. . In Manual of Clinical Microbiology, , 5th edn., pp. 12681270. Edited by Ballows A., Hauser W. J., Herrmann K. L., Isenberg H. D., Shadomy H. J. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Nie G. X., Ming H., Li S., Zhou E. M., Cheng J., Tang X., Feng H. G., Tang S. K., Li W. J. ( 2012 ). Amycolatopsis dongchuanensis sp. nov., an actinobacterium isolated from soil. . Int J Syst Evol Microbiol 62, 26502656. [CrossRef] [PubMed]
    [Google Scholar]
  24. Saintpierre-Bonaccio D., Amir H., Pineau R., Tan G. Y. A., Goodfellow M. ( 2005 ). Amycolatopsis plumensis sp. nov., a novel bioactive actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil. . Int J Syst Evol Microbiol 55, 20572061. [CrossRef] [PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  26. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  27. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [CrossRef]
    [Google Scholar]
  28. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. ( 1997 ). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47, 479491. [CrossRef]
    [Google Scholar]
  29. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  30. Takahashi Y. ( 2001 ). Family Pseudonocardiaceae.. In Identification Manual of Actinomycetes, pp. 227239. The Society for Actinomycetes Japan . Tokyo:: The Business Centre for Academic Societies;.
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  33. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  34. Warwick S., Bowen T., McVeigh H., Embley T. M. ( 1994 ). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia . . Int J Syst Bacteriol 44, 293299. [CrossRef] [PubMed]
    [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 17431813.[PubMed]
    [Google Scholar]
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