1887

Abstract

An aerobic, Gram-stain-negative, spiral or rod-shaped, non-spore-forming, diazotrophic bacterium (strain CC-LY743) was isolated from a fermentative tank in Taiwan. Strain CC-LY743 was able to grow at 20–37 °C and pH 6.0–8.0 and tolerated up to 3.0 % (w/v) NaCl. It was positive for nitrogen fixation, with activity of 10.6 nmol ethylene h. 16S rRNA gene sequence analysis of strain CC-LY743 showed highest similarity to DSM 19922 (96.1 %), JCM 21588 (96.0 %) and DSM 19657 (96.0 %) and lower similarity (<96.0 %) to all other species. Highest gene sequence similarities were obtained with BCRC 12270 (92.0 %), BCRC 80273 (92.3 %) and DSM 19657 (91.8 %). It was positive in the rapid identification by a genus-specific primer set. The predominant quinone system was ubiquinone 10 (Q-10) and the DNA G+C content was 69.6±0.1 mol%. The major fatty acids found in strain CC-LY743 were -C, C cyclo ω8, C 3-OH/C iso I, Cω7/Cω6 and Cω7/Cω6. Based on its phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY743 is considered to represent a novel species within the genus for which the name sp. nov. is proposed. The type strain is CC-LY743 ( = BCRC 80505 = JCM 18688 = LMG 27264).

Funding
This study was supported by the:
  • National Science Council
  • Council of Agriculture
  • Executive Yuan
  • Ministry of Education, Taiwan, ROC
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2013-10-01
2021-10-23
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