1887

Abstract

A bacterial strain designated RLAHU4B was isolated from root nodules of in León (Spain). The 16S rRNA gene sequence of this strain showed similarities lower than 97 % with respect to species of the genus . The strain was a Gram-variable, sporulating rod, motile by means of peritrichous flagella, and facultatively anaerobic. It was positive for oxidase, catalase and β-galactosidase production but negative for urease, amylase and gelatinase. Strain RLAHU4B grew in the presence of 5 % NaCl. MK-7 was the predominant menaquinone and -diaminopimelic acid was present in the peptidoglycan. anteiso-C, iso-C, iso-C and C were the major fatty acids. Major polar lipids of strain RLAHU4B were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, two unknown aminophospholipids and one unknown lipid. The DNA G+C content was 57.8 mol%. Strain RLAHU4B presented phenotypic differences from all recognized species of the genus . The phylogenetic, chemotaxonomic and phenotypic data indicated that strain RLAHU4B belongs to a novel species of the genus , for which the name sp. nov. is proposed, with strain RLAHU4B ( = LMG 27416 = CECT 8236) as the type strain.

Funding
This study was supported by the:
  • , MINECO (Spanish Central Government) , (Award AGL2010-17380)
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2014-01-01
2021-03-05
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