1887

Abstract

A novel psychrotolerant, strictly aerobic, non-motile, rod-shaped bacterial strain, designated IM13, was isolated from a sample taken from prehistoric guano paintings in Magura Cave, northwest Bulgaria and subjected to a polyphasic taxonomic study. Strain IM13 formed yellow colonies on LB agar plates and was Gram-staining-negative, heterotrophic and alkalitolerant. It grew optimally at pH 7.5 and 30 °C in the absence of NaCl. Phylogenetic analysis of the whole 16S rRNA gene revealed that strain IM13 branched with representatives of the genus with sequence similarity of 93–94 % with other species of the genus. The novel isolate contained iso-C (49.1 %), iso-Cω9 (18.2 %) and iso-C 3-OH (14.0 %) as dominant fatty acids. The DNA G+C content of strain IM13 was 33.5 mol%. Based on phylogenetic inference and phenotypic characteristics, it was concluded that strain IM13 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IM13 ( = DSM 26542 = NBIMCC 8736).

Funding
This study was supported by the:
  • Ministry of Education and Science of Bulgaria (Award DDVU 02/73)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.050831-0
2013-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4266.html?itemId=/content/journal/ijsem/10.1099/ijs.0.050831-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  3. Cho S.-H., Chae S.-H., Im W.-T., Kim S. B. ( 2011 ). Myroides marinus sp. nov., a member of the family Flavobacteriaceae, isolated from seawater. . Int J Syst Evol Microbiol 61, 938941. [View Article] [PubMed]
    [Google Scholar]
  4. Hugo C. J., Bruun B., Jooste P. J. ( 2006 ). The genera Empedobacter and Myroides . . In The Prokaryotes: A Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 630637. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  5. Mesbah M., Premachandran U., Whitman W. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  6. Smibert R., Krieg N. ( 1981 ). General characterization. . In Manual of Methods for General Bacteriology, pp. 409443. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA–DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. . Int J Syst Evol Microbiol 44, 846849.
    [Google Scholar]
  8. Stutzer M., Kwaschnina A. ( 1929 ). Aussaaten aus den Fäzes des Menschen gelbe Kolonien bildende Bakterien (Gattung Flavobacterium u.a.). . Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt 1 Orig 113, 219225.
    [Google Scholar]
  9. Tomova I., Lazarkevich I., Tomova A., Kambourova M., Vasileva-Tonkova E. ( 2013 ). Diversity and biosynthetic potential of culturable aerobic heterotrophic bacteria isolated from Magura Cave, Bulgaria. . Int J Speleol 42, 6576. [View Article]
    [Google Scholar]
  10. Vancanneyt M., Segers P., Torck U., Hoste B., Bernardet J.-F., Vandamme P., Kersters K. ( 1996 ). Reclassification of Flavobacterium odoratum (Stutzer 1929) strains to a new genus, Myroides, as Myroides odoratus comb. nov. and Myroides odoratiminus sp. nov.. Int J Syst Bacteriol 46, 926932. [View Article]
    [Google Scholar]
  11. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  12. Yan S., Zhao N., Zhang X.-H. ( 2012 ). Myroides phaeus sp. nov., isolated from human saliva, and emended descriptions of the genus Myroides and the species Myroides profundi Zhang et al. 2009 and Myroides marinus Cho et al. 2011. . Int J Syst Evol Microbiol 62, 770775. [View Article] [PubMed]
    [Google Scholar]
  13. Yoon J., Maneerat S., Kawai F., Yokota A. ( 2006 ). Myroides pelagicus sp. nov., isolated from seawater in Thailand. . Int J Syst Evol Microbiol 56, 19171920. [View Article] [PubMed]
    [Google Scholar]
  14. Zhang X. Y., Zhang Y. J., Chen X. L., Qin Q. L., Zhao D. L., Li T. G., Dang H. Y., Zhang Y. Z. ( 2008 ). Myroides profundi sp. nov., isolated from deep-sea sediment of the southern Okinawa Trough. . FEMS Microbiol Lett 287, 108112. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.050831-0
Loading
/content/journal/ijsem/10.1099/ijs.0.050831-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error