1887

Abstract

A novel psychrotolerant, strictly aerobic, non-motile, rod-shaped bacterial strain, designated IM13, was isolated from a sample taken from prehistoric guano paintings in Magura Cave, northwest Bulgaria and subjected to a polyphasic taxonomic study. Strain IM13 formed yellow colonies on LB agar plates and was Gram-staining-negative, heterotrophic and alkalitolerant. It grew optimally at pH 7.5 and 30 °C in the absence of NaCl. Phylogenetic analysis of the whole 16S rRNA gene revealed that strain IM13 branched with representatives of the genus with sequence similarity of 93–94 % with other species of the genus. The novel isolate contained iso-C (49.1 %), iso-Cω9 (18.2 %) and iso-C 3-OH (14.0 %) as dominant fatty acids. The DNA G+C content of strain IM13 was 33.5 mol%. Based on phylogenetic inference and phenotypic characteristics, it was concluded that strain IM13 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IM13 ( = DSM 26542 = NBIMCC 8736).

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2013-11-01
2019-10-19
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